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Detection of Group A Streptococcus in Pharyngeal Swab Specimens by Use of the AmpliVue GAS Isothermal Helicase-Dependent Amplification Assay. J Clin Microbiol 2015 Jul;53(7):2365-7

Date

05/15/2015

Pubmed ID

25972419

Pubmed Central ID

PMC4473221

DOI

10.1128/JCM.01085-15

Scopus ID

2-s2.0-84932620912 (requires institutional sign-in at Scopus site)   13 Citations

Abstract

We evaluated the clinical performance (sensitivity and specificity) of the AmpliVue group A Streptococcus (GAS) isothermal helicase-dependent amplification assay using 1,192 pharyngeal swab specimens. AmpliVue GAS assay results were compared to the results of routine throat cultures on selective streptococcal blood agar plates. The sensitivity and specificity of the AmpliVue GAS assay were 98.3% (95% confidence interval [CI], 95 to 100%) and 93.2% (95% CI, 91 to 95%), respectively.

Author List

Faron ML, Ledeboer NA, Granato P, Daly JA, Pierce K, Pancholi P, Uphoff TS, Buchan BW

Authors

Blake W. Buchan PhD Professor in the Pathology department at Medical College of Wisconsin
Nathan A. Ledeboer PhD Chief, Professor in the Pathology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Humans
Molecular Diagnostic Techniques
Nucleic Acid Amplification Techniques
Pharynx
Sensitivity and Specificity
Streptococcal Infections
Streptococcus pyogenes