Detection of immunoglobulin/c-myc recombinations in mice that are resistant to plasmacytoma induction. Cancer Res 1996 Jan 15;56(2):419-23
Date
01/15/1996Pubmed ID
8542601Scopus ID
2-s2.0-0030057747 (requires institutional sign-in at Scopus site) 25 CitationsAbstract
Interchromosomal recombinations between c-myc and immunoglobulin sequences can be found in preneoplastic lesions (oil granulomata) during pristane-induced plasmacytoma development in susceptible BALB/cAn mice. In this study we used a more sensitive approach, hybridization-enriched templates with nested PCR, to detect microclones with Ig alpha/c-myc recombinations in oil granulomata of susceptible and resistant mice. Recombinations were detected in as many as 73% (32/44) of plasmacytoma-susceptible BALB/cAn mice 30 days after an injection of pristane. Mice that are resistant to plasmacytoma induction can also harbor recombination-positive cells, but these are less frequent [2/20 DBA/2N, 8/20 (BALB/cAn x DBA/2N)F1, hereafter called CD2F1]. The clones in DBA/2N mice were small (< 400 cells), whereas in BALB/cAn, the oil granuloma harbored up to several thousand of these cells. We conclude that the molecular machinery for generating characteristic interchromosomal recombinations can be found in all strains of mice. Both the frequency of generating Ig alpha/c-myc recombinations and the expansion of recombination-positive cells are greater in susceptible mice than in resistant strains.
Author List
Müller JR, Jones GM, Potter M, Janz SAuthor
Siegfried Janz MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBase Sequence
Carcinogens
Clone Cells
DNA, Neoplasm
Disease Susceptibility
Genes, Immunoglobulin
Genes, myc
Mice
Mice, Inbred BALB C
Mice, Inbred DBA
Molecular Sequence Data
Plasmacytoma
Polymerase Chain Reaction
Precancerous Conditions
Recombination, Genetic
Sensitivity and Specificity
Terpenes
