Functional role of epoxyeicosatrienoic acids and their production in astrocytes: approaches for gene transfer and therapy (review). Int J Mol Med 1998 Dec;2(6):661-9
Date
12/16/1998Pubmed ID
9850733DOI
10.3892/ijmm.2.6.661Scopus ID
2-s2.0-0032238183 (requires institutional sign-in at Scopus site) 22 CitationsAbstract
The list of functions attributed to astrocytes in the brain is ever increasing. These cells contain cytochrome P450 enzymes that have recently demonstrated a number of exciting roles besides detoxification. The P450 monooxygenases can covert the substrate arachidonic acid to epoxyeicosatrienoic acids (EETs), metabolites that mediate vasodilation, mitogenesis, platelet aggregation, Ca2+ signaling and steroidogenesis. Integration of other physiological pathways present in astrocytes with P450 mediated EET formation has generated a number of interesting hypotheses to yield deeper insight into the role of astrocytes in the brain. In order to test these hypotheses as well as to enhance the benefits of EETs in astrocytes, we have used viral-mediated gene transfer and overexpression of one cytochrome P450 monooxygenase, 2C11, to engineer astrocytes for gene manipulation and possible gene therapy. This review outlines evidence for the presence of EETs in astrocytes, the function of EETs and the progress made with viral vectors expressing epoxygenase for gene manipulation in astrocytes.
Author List
Medhora M, Harder DMESH terms used to index this publication - Major topics in bold
8,11,14-Eicosatrienoic AcidAdenoviridae
Animals
Astrocytes
Cytochrome P-450 Enzyme System
Gene Transfer Techniques
Genetic Therapy
Genetic Vectors
Humans
Oxygenases
Platelet Aggregation
Signal Transduction
Steroids
Vasodilation
