Medical College of Wisconsin
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Xuelin Lou PhD


Institution: Medical College of Wisconsin
Department: Cell Biology, Neurobiology and Anatomy
Program: Administration

Member of the Cancer Center
Member of the Neuroscience Research Center

I am willing to collaborate on research projects.
I am willing to mentor a junior faculty member.

Research Areas of Interest

  • Super-resolution imaging, Nanoscopy
  • Synapse, synapse degeneration, Neurodegenerative diseases (Alzheimer's disease and Parkinson's disease)
  • Pancreatic islet cells, Diabetes
  • Membrane trafficking
  • Research Experience

  • Actin Cytoskeleton
  • Calcium Signaling
  • Diabetes Mellitus, Type 2
  • Endocytosis
  • Exocytosis
  • Ion Channels
  • Islets of Langerhans
  • Mice, Knockout
  • Neuronal Plasticity
  • Phosphatidylinositols
  • Presynaptic Terminals
  • Single Molecule Imaging
  • Synapses
  • Membrane biophysics: membrane fusion, membrane fission
  • Autophagy
  • Super resolution imaging
  • Neurodegeneration
  • mitochondria
  • vesicle trafficking
  • synaptopathy (for AD, PD, Autism, CMT)
  • Dynamin
  • Methodologies and Techniques

  • Molecular Biology
  • Patch-Clamp Techniques
  • Beta cells
  • Capacitance recording
  • Electron microscopy and tomography
  • Neurons
  • mouse genetics
  • Super-resolution microscopy (PALM, dSTORM, SIM, STED)
  • Live cell imaging: spinning disk confocal, TIRFM, FRET
  • sub-cellular optogenetics
  • Leadership Positions

  • Director, Advanced Cell Imaging Core
  • Publications (33)

  • Dynamin deficiency causes insulin secretion failure and hyperglycemia. (Fan F, Wu Y, Hara M, Rizk A, Ji C, Nerad D, Tamarina N, Lou X) Proc Natl Acad Sci U S A 2021 Aug 10;118(32) PMID: 34362840 PMCID: PMC8364113 SCOPUS ID: 2-s2.0-85112629945 08/08/2021       6 Citations
  • Imaging the Nanoscale Distribution of Phosphoinositides in the Cell Plasma Membrane with Single-Molecule Localization Super-Resolution Microscopy. (Fan F, Ji C, Lou X) Methods Mol Biol 2021;2251:91-104 PMID: 33481233 SCOPUS ID: 2-s2.0-85100326544 01/23/2021    
  • Real-Time Endocytosis Measurements by Membrane Capacitance Recording at Central Nerve Terminals. (Lou X) Methods Mol Biol 2018;1847:95-108 PMID: 30129012 SCOPUS ID: 2-s2.0-85052248659 08/22/2018    
  • Sensing Exocytosis and Triggering Endocytosis at Synapses: Synaptic Vesicle Exocytosis-Endocytosis Coupling. (Lou X) Front Cell Neurosci 2018;12:66 PMID: 29593500 PMCID: PMC5861208 SCOPUS ID: 2-s2.0-85046688018 03/30/2018       25 Citations
  • Using novel imaging approaches to study phosphoinositide signaling in vesicle trafficking (Lou X) Protein-Lipid Interactions: Perspectives, Techniques and Challenges 1 January 2018:107-132 SCOPUS ID: 2-s2.0-85049016121 01/01/2018    
  • Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. (Ji C, Fan F, Lou X) Cell Rep 2017 Aug 08;20(6):1409-1421 PMID: 28793264 PMCID: PMC5613661 SCOPUS ID: 2-s2.0-85026870735 08/10/2017       17 Citations
  • Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes. (Ji C, Lou X) J Vis Exp 2016 Oct 15(116) PMID: 27805608 PMCID: PMC5092206 SCOPUS ID: 2-s2.0-84992505061 11/03/2016       7 Citations
  • Dynamin 1- and 3-Mediated Endocytosis Is Essential for the Development of a Large Central Synapse In Vivo. (Fan F, Funk L, Lou X) J Neurosci 2016 Jun 01;36(22):6097-115 PMID: 27251629 PMCID: PMC4887570 SCOPUS ID: 2-s2.0-84971673132 06/03/2016       30 Citations
  • Dynamin-1 deletion enhances post-tetanic potentiation and quantal size after tetanic stimulation at the calyx of Held. (Mahapatra S, Lou X) J Physiol 2017 Jan 01;595(1):193-206 PMID: 27229184 PMCID: PMC5199734 SCOPUS ID: 2-s2.0-84977537713 05/28/2016       6 Citations
  • Tissue-specific dynamin-1 deletion at the calyx of Held decreases short-term depression through a mechanism distinct from vesicle resupply. (Mahapatra S, Fan F, Lou X) Proc Natl Acad Sci U S A 2016 May 31;113(22):E3150-8 PMID: 27185948 PMCID: PMC4896691 SCOPUS ID: 2-s2.0-84971572911 05/18/2016       15 Citations
  • Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis. (Fan F, Ji C, Wu Y, Ferguson SM, Tamarina N, Philipson LH, Lou X) J Clin Invest 2015 Nov 02;125(11):4026-41 PMID: 26413867 PMCID: PMC4639984 SCOPUS ID: 2-s2.0-84946760942 09/29/2015       31 Citations
  • Nanoscale Landscape of Phosphoinositides Revealed by Specific Pleckstrin Homology (PH) Domains Using Single-molecule Superresolution Imaging in the Plasma Membrane. (Ji C, Zhang Y, Xu P, Xu T, Lou X) J Biol Chem 2015 Nov 06;290(45):26978-26993 PMID: 26396197 PMCID: PMC4646391 SCOPUS ID: 2-s2.0-84946780726 09/24/2015       43 Citations
  • Reduced release probability prevents vesicle depletion and transmission failure at dynamin mutant synapses. (Lou X, Fan F, Messa M, Raimondi A, Wu Y, Looger LL, Ferguson SM, De Camilli P) Proc Natl Acad Sci U S A 2012 Feb 21;109(8):E515-23 PMID: 22308498 PMCID: PMC3286982 SCOPUS ID: 2-s2.0-84863118882 02/07/2012       39 Citations
  • Recruitment of endophilin to clathrin-coated pit necks is required for efficient vesicle uncoating after fission. (Milosevic I, Giovedi S, Lou X, Raimondi A, Collesi C, Shen H, Paradise S, O'Toole E, Ferguson S, Cremona O, De Camilli P) Neuron 2011 Nov 17;72(4):587-601 PMID: 22099461 PMCID: PMC3258500 SCOPUS ID: 2-s2.0-81355153885 11/22/2011       252 Citations
  • Overlapping role of dynamin isoforms in synaptic vesicle endocytosis. (Raimondi A, Ferguson SM, Lou X, Armbruster M, Paradise S, Giovedi S, Messa M, Kono N, Takasaki J, Cappello V, O'Toole E, Ryan TA, De Camilli P) Neuron 2011 Jun 23;70(6):1100-14 PMID: 21689597 PMCID: PMC3190241 SCOPUS ID: 2-s2.0-79959316429 06/22/2011       158 Citations
  • Regulation of transmitter release by Ca(2+) and synaptotagmin: insights from a large CNS synapse. (Kochubey O, Lou X, Schneggenburger R) Trends Neurosci 2011 May;34(5):237-46 PMID: 21439657 SCOPUS ID: 2-s2.0-79955731559 03/29/2011       68 Citations
  • Selective saturation of slow endocytosis at a giant glutamatergic central synapse lacking dynamin 1. (Lou X, Paradise S, Ferguson SM, De Camilli P) Proc Natl Acad Sci U S A 2008 Nov 11;105(45):17555-60 PMID: 18987309 PMCID: PMC2579887 SCOPUS ID: 2-s2.0-56249083913 11/07/2008       52 Citations
  • Phorbol esters modulate spontaneous and Ca2+-evoked transmitter release via acting on both Munc13 and protein kinase C. (Lou X, Korogod N, Brose N, Schneggenburger R) J Neurosci 2008 Aug 13;28(33):8257-67 PMID: 18701688 PMCID: PMC6670566 SCOPUS ID: 2-s2.0-51149097917 08/15/2008       100 Citations
  • Posttetanic potentiation critically depends on an enhanced Ca(2+) sensitivity of vesicle fusion mediated by presynaptic PKC. (Korogod N, Lou X, Schneggenburger R) Proc Natl Acad Sci U S A 2007 Oct 02;104(40):15923-8 PMID: 17884983 PMCID: PMC2000442 SCOPUS ID: 2-s2.0-35649003381 09/22/2007       70 Citations
  • A mechanism intrinsic to the vesicle fusion machinery determines fast and slow transmitter release at a large CNS synapse. (Wölfel M, Lou X, Schneggenburger R) J Neurosci 2007 Mar 21;27(12):3198-210 PMID: 17376981 PMCID: PMC6672471 SCOPUS ID: 2-s2.0-33947544508 03/23/2007       94 Citations
  • A Munc13/RIM/Rab3 tripartite complex: from priming to plasticity? (Dulubova I, Lou X, Lu J, Huryeva I, Alam A, Schneggenburger R, Südhof TC, Rizo J) EMBO J 2005 Aug 17;24(16):2839-50 PMID: 16052212 PMCID: PMC1187938 SCOPUS ID: 2-s2.0-28844449520 07/30/2005       201 Citations
  • Allosteric modulation of the presynaptic Ca2+ sensor for vesicle fusion. (Lou X, Scheuss V, Schneggenburger R) Nature 2005 May 26;435(7041):497-501 PMID: 15917809 SCOPUS ID: 2-s2.0-19444365446 05/27/2005       221 Citations
  • Presynaptic Ca2+ requirements and developmental regulation of posttetanic potentiation at the calyx of Held. (Korogod N, Lou X, Schneggenburger R) J Neurosci 2005 May 25;25(21):5127-37 PMID: 15917453 PMCID: PMC6724817 SCOPUS ID: 2-s2.0-19444371246 05/27/2005       83 Citations
  • Protein kinase activation increases insulin secretion by sensitizing the secretory machinery to Ca2+. (Wan QF, Dong Y, Yang H, Lou X, Ding J, Xu T) J Gen Physiol 2004 Dec;124(6):653-62 PMID: 15572345 PMCID: PMC2234026 SCOPUS ID: 2-s2.0-10344258585 12/02/2004       103 Citations
  • [Single channel and whole cell recordings using patch clamp technique]. (Lou XL, Zhou Z, Kang HG) Zhongguo Yi Liao Qi Xie Za Zhi 2000 Jul;24(4):221-6, 205 PMID: 12583139 SCOPUS ID: 2-s2.0-0141677863 02/14/2003       2 Citations
  • Na+ channel inactivation: a comparative study between pancreatic islet beta-cells and adrenal chromaffin cells in rat. (Lou XL, Yu X, Chen XK, Duan KL, He LM, Qu AL, Xu T, Zhou Z) J Physiol 2003 Apr 01;548(Pt 1):191-202 PMID: 12576496 PMCID: PMC2342793 SCOPUS ID: 2-s2.0-0037385010 02/11/2003       33 Citations
  • [A brief introduction to the secretion mechanism in immunocytes]. (Lou XL, He LM, Gong FL, Yu X, Xu T, Zhou Z) Sheng Li Xue Bao 2002 Jun 25;54(3):183-8 PMID: 12075462 SCOPUS ID: 2-s2.0-0039486492 06/21/2002    
  • The molecular mechanisms of insulin secretion and its regulation (Wu ZX, Lou XL, Qu AL, Zhou Z, Xu T) Progress in Biochemistry and Biophysics June 2002;29(3):342-347 SCOPUS ID: 2-s2.0-0036591324 06/01/2002       1 Citation
  • Evaluation of beta-amyloid peptide 25-35 on calcium homeostasis in cultured rat dorsal root ganglion neurons. (He LM, Chen LY, Lou XL, Qu AL, Zhou Z, Xu T) Brain Res 2002 Jun 07;939(1-2):65-75 PMID: 12020852 SCOPUS ID: 2-s2.0-0037036053 05/22/2002       32 Citations
  • [Ion channels in rat pancreatic beta cells]. (Zeng XH, Lou XL, Qu AL, Wu HX, Zhou Z) Sheng Li Xue Bao 2000 Apr;52(2):98-102 PMID: 11961576 SCOPUS ID: 2-s2.0-0347741450 04/19/2002       2 Citations
  • Glucose-induced Ca2+ signals in rat pancreatic β cells (Lou X, Zeng X, Qu A, Zhou Z) Progress in Natural Science 2001;11(2):113-114 SCOPUS ID: 2-s2.0-21444432900 01/01/2001       1 Citation
  • Different stimulatory opioid effects on intracellular Ca(2+) in SH-SY5Y cells. (Chen L, Zou S, Lou X, Kang HG) Brain Res 2000 Nov 03;882(1-2):256-65 PMID: 11056210 SCOPUS ID: 2-s2.0-0034602256 11/01/2000       13 Citations
  • Ca2+ signals induced from calcium stores in pancreatic islet β cells (Zeng X, Qu A, Lou X, Wang J, Wu H, Zhou Z) Chinese Science Bulletin 2000;45(1):51-56 SCOPUS ID: 2-s2.0-0034484818 01/01/2000       5 Citations
  • Last update: 09/08/2023