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Detection of recombinations between c-myc and immunoglobulin switch alpha in murine plasma cell tumors and preneoplastic lesions by polymerase chain reaction. Proc Natl Acad Sci U S A 1993 Aug 01;90(15):7361-5

Date

08/01/1993

Pubmed ID

8346257

Pubmed Central ID

PMC47137

DOI

10.1073/pnas.90.15.7361

Abstract

Virtually all murine plasmacytomas carry chromosomal translocations that activate c-myc. The predominant (approximately 90%) c-myc-activating chromosomal translocation in pristane (2,6,10,14-tetramethylpentadecane)-induced plasmacytomas in BALB/c mice is a reciprocal translocation t(12;15) in which an immunoglobulin heavy-chain switch sequence is joined to the 5' region of c-myc. The most common switch region involved is S alpha. We developed a direct PCR method to screen for recombinations between c-myc and S alpha. The critical step in establishing the method was the cloning and sequencing of the 5' flank of C alpha, a region with a reduced number of switch repeats that is much more favorable for designing specific PCR primers than the highly repetitive S alpha region. In applying this PCR method, we detected translocation-specific junction fragments in transplanted (10/16, 63%) and primary (5/15, 33%) plasmacytomas. Moreover, the sensitivity of a nested version of that technique allowed us to discern rare t(12;15)s in BALB/c mice in the preneoplastic stage of plasmacytoma-genesis (8/20 mice, 40%) as early as 30 days after administration of pristane. We conclude that t(12;15) is the probable primary, if not initiating, oncogenic step in plasmacytomagenesis.

Author List

Janz S, Müller J, Shaughnessy J, Potter M

Author

Siegfried Janz MD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Base Sequence
Genes, Immunoglobulin
Genes, Switch
Genes, myc
Immunoglobulin Heavy Chains
Immunoglobulin Isotypes
Immunoglobulin alpha-Chains
Mice
Molecular Sequence Data
Oligodeoxyribonucleotides
Plasmacytoma
Polymerase Chain Reaction
Precancerous Conditions
Recombination, Genetic
Translocation, Genetic
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