Faculty Collaboration Database

Endogenous glutamatergic inputs to the Parabrachial Nucleus/Kölliker-Fuse Complex determine respiratory rate. Respir Physiol Neurobiol 2020 Jun;277:103401

Date

02/10/2020

Pubmed ID

32036030

Pubmed Central ID

PMC7309965

DOI

10.1016/j.resp.2020.103401

Scopus ID

2-s2.0-85083237275 (requires institutional sign-in at Scopus site)   22 Citations

Abstract

The Kölliker-Fuse Nucleus (KF) has been widely investigated for its contribution to "inspiratory off-switch" while more recent studies showed that activation of the Parabrachial Nucleus (PBN) shortened expiratory duration. This study used an adult, in vivo, decerebrate rabbit model to delineate the contribution of each site to inspiratory and expiratory duration through sequential block of glutamatergic excitation with the receptor antagonists 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX) and d(-)-2-amino-5-phosphonopentanoic acid (AP5). Glutamatergic disfacilitation caused large increases in inspiratory and expiratory duration and minor decrease in peak phrenic activity (PPA). Hypoxia only partially reversed respiratory rate depression but PPA was increased to >200 % of control. The contribution of PBN activity to inspiratory and expiratory duration was equal while block of the KF affected inspiratory duration more than expiratory. We conclude that in the in vivo preparation respiratory rate greatly depends on PBN/KF activity, which contributes to the "inspiratory on- "and "off-switch", but is of minor importance for the magnitude of phrenic motor output.

Author List

Navarrete-Opazo AA, Cook-Snyder DR, Miller JR, Callison JJ, McCarthy N, Palkovic B, Stuth EAE, Zuperku EJ, Stucke AG

Authors

Denise R. Cook-Snyder PhD Associate Professor in the Physiology department at Medical College of Wisconsin
Astrid G. Stucke MD Professor in the Anesthesiology department at Medical College of Wisconsin
Eckehard A. Stuth MD Professor in the Anesthesiology department at Medical College of Wisconsin
Edward J. Zuperku PhD Professor in the Anesthesiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Excitatory Amino Acid Agonists
Excitatory Amino Acid Antagonists
Female
Glutamic Acid
Kolliker-Fuse Nucleus
Male
Microinjections
Parabrachial Nucleus
Quinoxalines
Rabbits
Respiratory Center
Respiratory Rate
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid