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Vasodilatory and toxic effects of spin traps on aerobic cardiac function. Free Radic Biol Med 1993 Feb;14(2):127-37

Date

02/01/1993

Pubmed ID

8425719

DOI

10.1016/0891-5849(93)90003-d

Scopus ID

2-s2.0-0027473299   64 Citations

Abstract

The objective of this study was to compare the effect of several structurally related nitrone and nitroso spin traps on the function of the isolated bicarbonate-buffer perfused rat heart model. Spin traps investigated were alpha-phenyl-tert-butyl N-nitrone (PBN), alpha-(4-pyridyl-1-oxide)-N-tert-butyl nitrone (POBN), 2-methyl-2-nitroso propane (MNP), 2-hydroxymethyl-2-nitroso propane (MNP/OH), nitrosobenzene (NB), dibromonitrosobenzene-sulfonic acid (DBNBS), and 5,5'-dimethyl-1-pyrroline-N-oxide (DMPO). During perfusion of hearts with increasing concentrations of spin traps, ventricular pressure, coronary flow rate, and heart rate were continuously recorded. The extent of contractile recovery was subsequently measured upon return to spin-trap free perfusion. The percentage of maximum increase in coronary flow with PBN, POBN, MNP, MNP-OH, NB, DBNBS, and DMPO were 11, 40, 45, 66, 28, 28, and 29%, respectively. Thus, all nitroso and nitrone spin traps studied acted as vasodilators. Over the dose range studied, POBN, MNP, MNP/OH, and DMPO did not exert any chronotropic effect. PBN, NB, and DBNBS exerted a negative chronotropic effect at higher concentrations. All spin traps studied, with the exception of DMPO, exerted a negative inotropic effect at the higher concentrations studied. We conclude that all spin traps examined acted as coronary vasodilators. Their negative chronotropic and inotropic effects were minimal in comparison and only manifest at the higher concentrations studied.

Author List

Konorev EA, Baker JE, Joseph J, Kalyanaraman B

Authors

John E. Baker PhD Professor in the Surgery department at Medical College of Wisconsin
Balaraman Kalyanaraman PhD Chair, Professor in the Biophysics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Aerobiosis
Animals
Benzenesulfonates
Cardiovascular Physiological Phenomena
Cardiovascular System
Coronary Circulation
Cyclic N-Oxides
Depression, Chemical
Heart Rate
Heart Ventricles
Mannitol
Nitrogen Oxides
Nitroso Compounds
Perfusion
Pressure
Pyridines
Rats
Rats, Sprague-Dawley
Spin Labels
Vasodilation
Ventricular Function
jenkins-FCD Prod-482 91ad8a360b6da540234915ea01ff80e38bfdb40a