LncRNA00492 is required for marginal zone B-cell development. Immunology 2022 Jan;165(1):88-98
Date
08/27/2021Pubmed ID
34435359DOI
10.1111/imm.13408Scopus ID
2-s2.0-85114376236 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
B-cell development undergoes a series of steps from the bone marrow to the secondary lymphoid organs. A defect in B-cell development can lead to immunodeficiency or malignant disorders, such as leukaemia or lymphoma. Long non-coding RNAs have been reported to act as important regulators of many pathological processes. However, very little is known regarding the role of lncRNAs during B-cell development and the regulation of their expression. In this study, we explored the expression and role of lncRNA Gme00492 in B-cell development. We observed that lnc00492 was highly expressed in B-cell development and primarily expressed in the nucleus. Lnc00492-deficient mice had fewer marginal zone B cells in the spleen, likely due to a developmental block. Importantly, lnc00492 interacts with CTBP1 and targets it for ubiquitination and degradation during B-cell development, whereas the transcriptional corepressor factor CTBP1 plays a critical role in Notch2 signalling. Thus, we identified a novel regulatory axis between lnc00492 and CTBP1 in B cells, suggesting that lnc00492 is essential for marginal zone B-cell development.
Author List
Wang F, Cui D, Zhang Q, Shao Y, Zheng B, Chen L, Luo Y, Yuan L, Wang DAuthor
Demin Wang PhD Professor in the Microbiology and Immunology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Alcohol OxidoreductasesAnimals
B-Lymphocytes
Biomarkers
Bone Marrow
Cell Differentiation
DNA-Binding Proteins
Gene Expression Regulation
Immunophenotyping
Lymphopoiesis
Mice
Mice, Knockout
Models, Biological
Protein Binding
RNA, Long Noncoding
Receptor, Notch2
Signal Transduction
Spleen
Ubiquitination
