Methods for studying voltage-gated sodium channels in heterologous expression systems. Methods Mol Med 2006;129:163-85
Date
11/07/2006Pubmed ID
17085811DOI
10.1385/1-59745-213-0:163Scopus ID
2-s2.0-35548949165 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
The discovery that oocytes of the frog Xenopus laevis can be induced to express working membrane ion channels by introducing channel mRNA into their cytoplasm (heterologous expression) has greatly impacted the field of ion channel physiology. With the addition of site-directed mutagenesis techniques, the functional consequences of virtually any mutation can now be specifically and easily assessed. Here, we describe an effective procedure for investigating cardiac sodium channel gating (hNaV1.5) both in Xenopus oocytes, and in a mammalian expression system, human embryonic kidney (HEK) 293 cells. We describe cell attached patch clamp for oocytes, and whole cell voltage clamp in HEK 293 cells.
Author List
Dice MS, Kearl T, Ruben PCAuthor
Tyce J. Kearl MD, PhD Assistant Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCell Line
Electrodes
Electrophysiology
Gene Expression
Humans
Microinjections
Muscle Proteins
NAV1.5 Voltage-Gated Sodium Channel
Oocytes
Patch-Clamp Techniques
RNA, Messenger
Sodium Channels
Transfection
Xenopus laevis
