Faculty Collaboration Database

Protective effect of Lifor solution in experimental renal ischemia-reperfusion injury. J Surg Res 2010 Dec;164(2):e291-7

Date

10/05/2010

Pubmed ID

20888590

Pubmed Central ID

PMC2991539

DOI

10.1016/j.jss.2010.08.033

Scopus ID

2-s2.0-78449301861 (requires institutional sign-in at Scopus site)   30 Citations

Abstract

BACKGROUND: Improved kidney preservation methods are needed to reduce ischemia-reperfusion (IR) injury in kidney allografts. Lifor is an artificial preservation solution comprised of nutrients, growth factors, and a non-protein oxygen and nutrient carrier. The current study compared the effectiveness of Lifor to University of Wisconsin solution (UW) in protecting rat kidneys from warm IR and cold storage injury.

MATERIALS AND METHODS: In a warm IR model, rat kidneys were perfused in situ with either saline, UW, or Lifor for 45 min. Renal function and histology were assessed 24 h later. In a cold IR model, kidney slices were cold-stored in saline, UW, or Lifor at 4°C. Kidney injury was assessed by the release of lactate dehydrogenase (LDH) and immunoblot analysis for cleaved caspase-3.

RESULTS: Lifor perfusion significantly mitigated renal dysfunction and tubular injury at 24 h compared with saline or UW. Lifor and UW prevented LDH release in hypoxic kidney slices in vitro, however activation of caspase-3 following hypoxia-reoxygenation was attenuated only with Lifor. Cold storage with Lifor or UW significantly decreased LDH release from kidney slices or normal rat kidney cells in comparison to storage in saline or culture media. After 24 h of cold storage there was a significant decrease in cleaved caspase-3 in Lifor stored slices compared that seen following cold storage in saline or UW solution.

CONCLUSIONS: Lifor solution mitigates both warm and cold renal IR and appears to provide greater protection from apoptosis compared with UW solution.

Author List

Regner KR, Nilakantan V, Ryan RP, Mortensen J, White SM, Shames BD, Roman RJ

Author

Kevin R. Regner MD Interim Chair, Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Adenosine
Allopurinol
Animals
Caspase 3
Glutathione
In Situ Nick-End Labeling
Insulin
Kidney
Kidney Transplantation
L-Lactate Dehydrogenase
Male
Nephrectomy
Organ Preservation Solutions
Raffinose
Rats
Rats, Inbred Lew
Rats, Sprague-Dawley
Renal Circulation
Reperfusion Injury
Transplantation, Homologous