A common set of nuclear factors bind to promoter elements regulated by the retinoblastoma protein. Cell Growth Differ 1992 Sep;3(9):597-608
Date
09/01/1992Pubmed ID
1419910Scopus ID
2-s2.0-0026924150 (requires institutional sign-in at Scopus site) 34 CitationsAbstract
A 30-base pair element within the c-fos promoter, termed the RCE (retinoblastoma control element), has previously been shown to be the target of transcriptional regulation by the product of the retinoblastoma (Rb) gene. We have identified three nuclear proteins [retinoblastoma control proteins (RCPs)] that complex with this promoter element in vitro. The Rb gene does not appear to encode the RCPs as the expression of Rb in vivo does not correlate with RCE-RCP complex formation in vitro. A single binding site for the RCPs within the c-fos RCE was identified, and the nucleotides required for protein-DNA complex formation were defined. Similar sequences are found in the promoters of two additional genes that are regulated by Rb (c-myc and TGF-beta 1), and binding assays demonstrate that the RCPs also interact with these elements. Linkage of the c-fos RCE to the herpes simplex virus thymidine kinase promoter led to a 4-fold stimulation of expression in transient transfection assays. Mutations within the RCP binding site that abrogate stable interaction of the RCPs with the RCE in vitro block RCE transcriptional activity in vivo. Our results suggest a role for the RCPs in RCE-dependent transcription and the regulation of transcription by the Rb protein.
Author List
Udvadia AJ, Rogers KT, Horowitz JMAuthor
Ava Udvadia BS,PhD Associate Professor in the Biological Sciences department at University of Wisconsin - MilwaukeeMESH terms used to index this publication - Major topics in bold
Base SequenceDNA-Binding Proteins
Gene Expression Regulation
Genes, fos
Humans
Macromolecular Substances
Molecular Sequence Data
Mutation
Nuclear Proteins
Promoter Regions, Genetic
Proto-Oncogene Proteins c-fos
Repetitive Sequences, Nucleic Acid
Retinoblastoma Protein
Transcription Factors
Transcription, Genetic
