Faculty Collaboration Database

Fetomaternal cross talk in the placental vascular bed: control of coagulation by trophoblast cells. Blood 2006 Apr 15;107(8):3173-80

Date

12/29/2005

Pubmed ID

16380449

Pubmed Central ID

PMC1895751

DOI

10.1182/blood-2005-10-4111

Scopus ID

2-s2.0-33645746632 (requires institutional sign-in at Scopus site)   88 Citations

Abstract

Humans and rodents exhibit a peculiar type of placentation in which zygote-derived trophoblast cells, rather than endothelial cells, line the terminal maternal vascular space. This peculiar aspect of the placental vasculature raises important questions about the relative contribution of fetal and maternal factors in the local control of hemostasis in the placenta and how these might determine the phenotypic expression of thrombophilia-associated complications of pregnancy. Using genomewide expression analysis, we identify a panel of genes that determine the ability of fetal trophoblast cells to regulate hemostasis at the fetomaternal interface. We show that spontaneous differentiation of trophoblast stem cells is associated with the acquisition of an endothelial cell-like thromboregulatory gene expression program. This program is developmentally regulated and conserved between mice and humans. We further show that trophoblast cells sense, via the expression of protease activated receptors, the presence of activated coagulation factors. Engagement of these receptors results in cell-type specific changes in gene expression. Our observations define candidate fetal genes that are potential risk modifiers of maternal thrombophilia-associated pregnancy complications and provide evidence that coagulation activation at the fetomaternal interface can affect trophoblast physiology altering placental function in the absence of frank thrombosis.

Author List

Sood R, Kalloway S, Mast AE, Hillard CJ, Weiler H

Authors

Cecilia J. Hillard PhD Associate Dean, Center Director, Professor in the Pharmacology and Toxicology department at Medical College of Wisconsin
Rashmi Sood PhD Associate Professor in the Pathology department at Medical College of Wisconsin
Hartmut Weiler PhD Associate Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Blood Coagulation
Blood Coagulation Factors
Cell Differentiation
Endothelial Cells
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental
Hemostasis
Humans
Maternal-Fetal Exchange
Mice
Oligonucleotide Array Sequence Analysis
Pregnancy
Pregnancy Complications, Hematologic
Receptors, Cell Surface
Stem Cells
Thrombophilia
Thrombosis
Trophoblasts
Zygote