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Transcriptional inhibition of etv2 expression is essential for embryonic cardiac development. Dev Biol 2014 Sep 01;393(1):71-83

Date

07/02/2014

Pubmed ID

24984259

Pubmed Central ID

PMC4137469

DOI

10.1016/j.ydbio.2014.06.019

Scopus ID

2-s2.0-84905732091   11 Citations

Abstract

E-twenty six variant 2 (Etv2) transcription factor participates in cardiac, vascular-endothelial and blood cell lineage specification decisions during embryonic development. Previous studies have identified genomic elements in the etv2 locus responsible for vascular endothelial cell specification. Using transgenic analysis in zebrafish, we report here an etv2 proximal promoter fragment that prevents transgene misexpression in myocardial progenitor cells. This inhibition of etv2 expression in the cardiac progenitor population is partly mediated by Scl and Nkx2.5, likely through direct binding to the etv2 promoter, and cis-regulatory elements located in the first and second introns. The results identify an etv2 cis-regulatory mechanism controlling cardiovascular fate choice implying that etv2 participates in a transcriptional network mediating developmental plasticity of endothelial progenitor cells during embryonic development.

Author List

Schupp MO, Waas M, Chun CZ, Ramchandran R

Author

Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Animals, Genetically Modified
Basic Helix-Loop-Helix Transcription Factors
Cell Differentiation
Cell Line
Cell Lineage
Embryonic Stem Cells
Endothelial Cells
Endothelium, Vascular
Erythrocytes
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Gene Silencing
Heart
Homeobox Protein Nkx-2.5
Morpholinos
Promoter Regions, Genetic
Proto-Oncogene Proteins
T-Cell Acute Lymphocytic Leukemia Protein 1
Transcription Factors
Transcription, Genetic
Transgenes
Zebrafish
Zebrafish Proteins
jenkins-FCD Prod-484 8aa07fc50b7f6d102f3dda2f4c7056ff84294d1d