Transcriptional inhibition of etv2 expression is essential for embryonic cardiac development. Dev Biol 2014 Sep 01;393(1):71-83
Date
07/02/2014Pubmed ID
24984259Pubmed Central ID
PMC4137469DOI
10.1016/j.ydbio.2014.06.019Scopus ID
2-s2.0-84905732091 (requires institutional sign-in at Scopus site) 15 CitationsAbstract
E-twenty six variant 2 (Etv2) transcription factor participates in cardiac, vascular-endothelial and blood cell lineage specification decisions during embryonic development. Previous studies have identified genomic elements in the etv2 locus responsible for vascular endothelial cell specification. Using transgenic analysis in zebrafish, we report here an etv2 proximal promoter fragment that prevents transgene misexpression in myocardial progenitor cells. This inhibition of etv2 expression in the cardiac progenitor population is partly mediated by Scl and Nkx2.5, likely through direct binding to the etv2 promoter, and cis-regulatory elements located in the first and second introns. The results identify an etv2 cis-regulatory mechanism controlling cardiovascular fate choice implying that etv2 participates in a transcriptional network mediating developmental plasticity of endothelial progenitor cells during embryonic development.
Author List
Schupp MO, Waas M, Chun CZ, Ramchandran RAuthor
Ramani Ramchandran PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnimals, Genetically Modified
Basic Helix-Loop-Helix Transcription Factors
Cell Differentiation
Cell Line
Cell Lineage
Embryonic Stem Cells
Endothelial Cells
Endothelium, Vascular
Erythrocytes
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Gene Silencing
Heart
Homeobox Protein Nkx-2.5
Morpholinos
Promoter Regions, Genetic
Proto-Oncogene Proteins
T-Cell Acute Lymphocytic Leukemia Protein 1
Transcription Factors
Transcription, Genetic
Transgenes
Zebrafish
Zebrafish Proteins
