Faculty Collaboration Database

IL-10 secretion increases signal persistence of HEMA-MMA-microencapsulated luciferase-modified CHO fibroblasts in mice. Tissue Eng Part A 2009 Jan;15(1):127-36

Date

08/20/2008

Pubmed ID

18710337

DOI

10.1089/ten.tea.2008.0028

Scopus ID

2-s2.0-58149265302 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

Microencapsulation of cells in a polymer membrane [e.g., poly(hydroxyethyl methacrylate-co-methyl methacrylate) (HEMA-MMA)] has been proposed as a vehicle for the delivery of therapeutic biomolecules, but cells (especially xenogeneic cells) survive only for short times, limiting the utility of this approach. Murine interleukin-10 (mIL-10) has been shown to downregulate the xenogeneic immune response, and we tested the hypothesis that mIL-10 produced by microencapsulated Chinese hamster ovary (CHO) cells would modulate the transplant-site environment leading to prolonged cell function in a xenogeneic model without other immunomodulatory agents. Prior to encapsulation, CHO cells were genetically engineered to express mIL-10 and a firefly bioluminescence protein, luciferase, which allowed for noninvasive tracking of transplanted cells in vivo with the Xenogen IVIS Imaging System. This nondestructive imaging system was sufficiently sensitive to detect photon signal emitted by a single capsule containing around 800 luciferase-transduced CHO (CHO(LUC)) cells in vitro, and to track changes in luciferase expression in vivo over time. Effective modulation of the transplantation-site environment with mIL-10 secreted from capsules was evident by greater luciferase expression at 10 and 21 days after transplantation relative to encapsulated luciferase-transfected cells that did not produce mIL-10. Longer duration effects require further investigation to extend this proof-of-concept study.

Author List

Surzyn M, Symes J, Medin JA, Sefton MV

Author

Jeffrey A. Medin PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Biocompatible Materials
CHO Cells
Clone Cells
Cricetinae
Cricetulus
Drug Compounding
Fibroblasts
Genetic Vectors
Interleukin-10
Luciferases
Luminescent Agents
Male
Methacrylates
Methylmethacrylate
Mice
Mice, Inbred BALB C
Signal Transduction
Transfection
Transplantation, Heterologous