Structural basis of arrestin-3 activation and signaling. Nat Commun 2017 Nov 10;8(1):1427
Date
11/12/2017Pubmed ID
29127291Pubmed Central ID
PMC5681653DOI
10.1038/s41467-017-01218-8Scopus ID
2-s2.0-85033606940 (requires institutional sign-in at Scopus site) 90 CitationsAbstract
A unique aspect of arrestin-3 is its ability to support both receptor-dependent and receptor-independent signaling. Here, we show that inositol hexakisphosphate (IP6) is a non-receptor activator of arrestin-3 and report the structure of IP6-activated arrestin-3 at 2.4-Å resolution. IP6-activated arrestin-3 exhibits an inter-domain twist and a displaced C-tail, hallmarks of active arrestin. IP6 binds to the arrestin phosphate sensor, and is stabilized by trimerization. Analysis of the trimerization surface, which is also the receptor-binding surface, suggests a feature called the finger loop as a key region of the activation sensor. We show that finger loop helicity and flexibility may underlie coupling to hundreds of diverse receptors and also promote arrestin-3 activation by IP6. Importantly, we show that effector-binding sites on arrestins have distinct conformations in the basal and activated states, acting as switch regions. These switch regions may work with the inter-domain twist to initiate and direct arrestin-mediated signaling.
Author List
Chen Q, Perry NA, Vishnivetskiy SA, Berndt S, Gilbert NC, Zhuo Y, Singh PK, Tholen J, Ohi MD, Gurevich EV, Brautigam CA, Klug CS, Gurevich VV, Iverson TMAuthor
Candice S. Klug PhD Professor in the Biophysics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Amino Acid SequenceAnimals
Arrestins
Binding Sites
Cattle
Crystallography, X-Ray
Humans
Mitogen-Activated Protein Kinase 10
Models, Molecular
Phytic Acid
Protein Conformation
Protein Structure, Quaternary
Recombinant Proteins
Signal Transduction