Faculty Collaboration Database

Murine CCR9, a chemokine receptor for thymus-expressed chemokine that is up-regulated following pre-TCR signaling. J Immunol 2000 Jan 15;164(2):639-48

Date

01/07/2000

Pubmed ID

10623805

DOI

10.4049/jimmunol.164.2.639

Scopus ID

2-s2.0-0034650922 (requires institutional sign-in at Scopus site)   95 Citations

Abstract

Chemokines are likely to play an important role in regulating the trafficking of developing T cells within the thymus. By using anti-CD3varepsilon treatment of recombinase-activating gene 2 (Rag2-/-) mice to mimic pre-TCR signaling and drive thymocyte development to the double positive stage, we have identified murine GPR-9-6 as a chemokine receptor whose expression is strongly induced following pre-TCR signaling. GPR-9-6 mRNA is present at high levels in the thymus, and by RT-PCR analysis its expression is induced as normal thymocytes undergo the double negative to double positive transition. Furthermore we show that TECK (thymus-expressed chemokine), a chemokine produced by thymic medullary dendritic cells, is a functional ligand for GPR-9-6. TECK specifically induces a calcium flux and chemotaxis of GPR-9-6-transfected cells. In addition, TECK stimulates the migration of normal double positive thymocytes, as well as Rag2-/- thymocytes following anti-CD3varepsilon treatment. Hence, GPR-9-6 has been designated as CC chemokine receptor 9 (CCR9). Our results suggest that TECK delivers signals through CCR9 important for the navigation of developing thymocytes.

Author List

Norment AM, Bogatzki LY, Gantner BN, Bevan MJ

Author

Benjamin N. Gantner PhD Assistant Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Monoclonal
Calcium
Cell Differentiation
Cell Line
Cell Movement
Chemokines, CC
Chemotaxis, Leukocyte
DNA-Binding Proteins
Mice
Mice, Inbred C57BL
Mice, Transgenic
Receptors, Antigen, T-Cell
Receptors, CCR
Receptors, Chemokine
Signal Transduction
Stem Cells
T-Lymphocyte Subsets
Thymus Gland
Transfection
Up-Regulation