Persistence of immunoglobulin heavy chain/c-myc recombination-positive lymphocyte clones in the blood of human immunodeficiency virus-infected homosexual men. Proc Natl Acad Sci U S A 1995 Jul 03;92(14):6577-81
Date
07/03/1995Pubmed ID
7604036Pubmed Central ID
PMC41561DOI
10.1073/pnas.92.14.6577Scopus ID
2-s2.0-0029056826 (requires institutional sign-in at Scopus site) 66 CitationsAbstract
We studied blood lymphocytes of human immunodeficiency virus (HIV)-seropositive and -negative homosexual men for the presence of T(8;14) translocations that recombine c-myc and immunoglobulin heavy-chain (IgH) mu/IgH alpha switch regions. Clones with T(8;14) translocations were detected in 10.5% (12/114) of the HIV-positive and in 2.0% of the 99 uninfected patients. The majority of recombinations were found at a single time point only. Four patients, however, harbored multiple (up to four) and persistent (up to 9 years) translocation-positive cell clones. No correlation between the presence of these aberrant lymphocytes and a later lymphoma could be established. The exon 1/intron 1 region of the recombined c-myc was investigated for the presence of point mutations and these were found in the nonpersistent clones. Additional alterations detected in these clones included duplications and a deletion in the c-myc gene. The pattern of base substitution indicates that they were introduced after the translocation event.
Author List
Müller JR, Janz S, Goedert JJ, Potter M, Rabkin CSAuthor
Siegfried Janz MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Base SequenceChromosome Mapping
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 8
Clone Cells
Cohort Studies
DNA Primers
Genes, myc
HIV Seronegativity
HIV Seropositivity
Homosexuality, Male
Humans
Immunoglobulin Heavy Chains
Lymphocytes
Male
Molecular Sequence Data
Polymerase Chain Reaction
Recombination, Genetic
Restriction Mapping
Translocation, Genetic
