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Persistence of immunoglobulin heavy chain/c-myc recombination-positive lymphocyte clones in the blood of human immunodeficiency virus-infected homosexual men. Proc Natl Acad Sci U S A 1995 Jul 03;92(14):6577-81

Date

07/03/1995

Pubmed ID

7604036

Pubmed Central ID

PMC41561

DOI

10.1073/pnas.92.14.6577

Scopus ID

2-s2.0-0029056826   65 Citations

Abstract

We studied blood lymphocytes of human immunodeficiency virus (HIV)-seropositive and -negative homosexual men for the presence of T(8;14) translocations that recombine c-myc and immunoglobulin heavy-chain (IgH) mu/IgH alpha switch regions. Clones with T(8;14) translocations were detected in 10.5% (12/114) of the HIV-positive and in 2.0% of the 99 uninfected patients. The majority of recombinations were found at a single time point only. Four patients, however, harbored multiple (up to four) and persistent (up to 9 years) translocation-positive cell clones. No correlation between the presence of these aberrant lymphocytes and a later lymphoma could be established. The exon 1/intron 1 region of the recombined c-myc was investigated for the presence of point mutations and these were found in the nonpersistent clones. Additional alterations detected in these clones included duplications and a deletion in the c-myc gene. The pattern of base substitution indicates that they were introduced after the translocation event.

Author List

Müller JR, Janz S, Goedert JJ, Potter M, Rabkin CS

Author

Siegfried Janz MD Professor in the Medicine department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 8
Clone Cells
Cohort Studies
DNA Primers
Genes, myc
HIV Seronegativity
HIV Seropositivity
Homosexuality, Male
Humans
Immunoglobulin Heavy Chains
Lymphocytes
Male
Molecular Sequence Data
Polymerase Chain Reaction
Recombination, Genetic
Restriction Mapping
Translocation, Genetic