Medical College of Wisconsin
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Micael Joel Hardy PhD

Visiting Assistant Professor

Institution: Medical College of Wisconsin
Department: Biophysics
Program: Research

Publications (51)

  • Chemoprevention of Lung Cancer with a Combination of Mitochondria-Targeted Compounds. (Zhang Q, Xiong D, Pan J, Wang Y, Hardy M, Kalyanaraman B, You M) Cancers (Basel) 2022 May 21;14(10) PMID: 35626143 PMCID: PMC9140024 SCOPUS ID: 2-s2.0-85130231561 05/29/2022    
  • Combining PEGylated mito-atovaquone with MCT and Krebs cycle redox inhibitors as a potential strategy to abrogate tumor cell proliferation. (Cheng G, Hardy M, You M, Kalyanaraman B) Sci Rep 2022 Mar 24;12(1):5143 PMID: 35332210 PMCID: PMC8948292 SCOPUS ID: 2-s2.0-85127039662 03/26/2022    
  • Prevention of Tumor Growth and Dissemination by In Situ Vaccination with Mitochondria-Targeted Atovaquone. (Huang M, Xiong D, Pan J, Zhang Q, Wang Y, Myers CR, Johnson BD, Hardy M, Kalyanaraman B, You M) Adv Sci (Weinh) 2022 Apr;9(12):e2101267 PMID: 35243806 PMCID: PMC9036031 SCOPUS ID: 2-s2.0-85125564839 03/05/2022       3 Citations
  • Mitochondria-targeted hydroxyurea inhibits OXPHOS and induces antiproliferative and immunomodulatory effects. (Cheng G, Hardy M, Topchyan P, Zander R, Volberding P, Cui W, Kalyanaraman B) iScience 2021 Jun 25;24(6):102673 PMID: 34189437 PMCID: PMC8215227 SCOPUS ID: 2-s2.0-85107809306 07/01/2021       7 Citations
  • Synchronous effects of targeted mitochondrial complex I inhibitors on tumor and immune cells abrogate melanoma progression. (AbuEid M, McAllister DM, McOlash L, Harwig MC, Cheng G, Drouillard D, Boyle KA, Hardy M, Zielonka J, Johnson BD, Hill RB, Kalyanaraman B, Dwinell MB) iScience 2021 Jun 25;24(6):102653 PMID: 34189432 PMCID: PMC8220235 SCOPUS ID: 2-s2.0-85107963806 07/01/2021       6 Citations
  • Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid. (Zielonka J, Sikora A, Podsiadly R, Hardy M, Kalyanaraman B) Methods Mol Biol 2021;2275:315-327 PMID: 34118047 PMCID: PMC9245525 SCOPUS ID: 2-s2.0-85108205869 06/13/2021       2 Citations
  • Oxidation of ethidium-based probes by biological radicals: mechanism, kinetics and implications for the detection of superoxide. (Michalski R, Thiebaut D, Michałowski B, Ayhan MM, Hardy M, Ouari O, Rostkowski M, Smulik-Izydorczyk R, Artelska A, Marcinek A, Zielonka J, Kalyanaraman B, Sikora A) Sci Rep 2020 Oct 29;10(1):18626 PMID: 33122809 PMCID: PMC7596101 SCOPUS ID: 2-s2.0-85094638056 10/31/2020       7 Citations
  • Potent inhibition of tumour cell proliferation and immunoregulatory function by mitochondria-targeted atovaquone. (Cheng G, Hardy M, Topchyan P, Zander R, Volberding P, Cui W, Kalyanaraman B) Sci Rep 2020 Oct 21;10(1):17872 PMID: 33087770 PMCID: PMC7578061 SCOPUS ID: 2-s2.0-85093870747 10/23/2020       16 Citations
  • Mitochondria-targeted magnolol inhibits OXPHOS, proliferation, and tumor growth via modulation of energetics and autophagy in melanoma cells. (Cheng G, Hardy M, Zielonka J, Weh K, Zielonka M, Boyle KA, Abu Eid M, McAllister D, Bennett B, Kresty LA, Dwinell MB, Kalyanaraman B) Cancer Treat Res Commun 2020;25:100210 PMID: 32987287 PMCID: PMC7883397 SCOPUS ID: 2-s2.0-85091576205 09/29/2020       7 Citations
  • Synergistic inhibition of tumor cell proliferation by metformin and mito-metformin in the presence of iron chelators. (Cheng G, Zielonka J, Hardy M, Ouari O, Chitambar CR, Dwinell MB, Kalyanaraman B) Oncotarget 2019 May 28;10(37):3518-3532 PMID: 31191823 PMCID: PMC6544408 SCOPUS ID: 2-s2.0-85089087803 06/14/2019       10 Citations
  • Embedding cyclic nitrone in mesoporous silica particles for EPR spin trapping of superoxide and other radicals. (Besson E, Gastaldi S, Bloch E, Zielonka J, Zielonka M, Kalyanaraman B, Aslan S, Karoui H, Rockenbauer A, Ouari O, Hardy M) Analyst 2019 Jul 21;144(14):4194-4203 PMID: 31180410 SCOPUS ID: 2-s2.0-85068588717 06/11/2019       12 Citations
  • High-Throughput Screening of NOX Inhibitors. (Zielonka J, Zielonka M, Cheng G, Hardy M, Kalyanaraman B) Methods Mol Biol 2019;1982:429-446 PMID: 31172487 PMCID: PMC6953630 SCOPUS ID: 2-s2.0-85067079084 06/07/2019       6 Citations
  • Targeting lonidamine to mitochondria mitigates lung tumorigenesis and brain metastasis. (Cheng G, Zhang Q, Pan J, Lee Y, Ouari O, Hardy M, Zielonka M, Myers CR, Zielonka J, Weh K, Chang AC, Chen G, Kresty L, Kalyanaraman B, You M) Nat Commun 2019 May 17;10(1):2205 PMID: 31101821 PMCID: PMC6525201 SCOPUS ID: 2-s2.0-85065824371 05/19/2019       102 Citations
  • Mitochondria-Targeted Honokiol Confers a Striking Inhibitory Effect on Lung Cancer via Inhibiting Complex I Activity. (Pan J, Lee Y, Cheng G, Zielonka J, Zhang Q, Bajzikova M, Xiong D, Tsaih SW, Hardy M, Flister M, Olsen CM, Wang Y, Vang O, Neuzil J, Myers CR, Kalyanaraman B, You M) iScience 2018 May 25;3:192-207 PMID: 30428319 PMCID: PMC6137433 SCOPUS ID: 2-s2.0-85063413951 11/15/2018       28 Citations
  • Detection of mitochondria-generated reactive oxygen species in cells using multiple probes and methods: Potentials, pitfalls, and the future. (Cheng G, Zielonka M, Dranka B, Kumar SN, Myers CR, Bennett B, Garces AM, Dias Duarte Machado LG, Thiebaut D, Ouari O, Hardy M, Zielonka J, Kalyanaraman B) J Biol Chem 2018 Jun 29;293(26):10363-10380 PMID: 29739855 PMCID: PMC6028982 SCOPUS ID: 2-s2.0-85049623107 05/10/2018       61 Citations
  • Corrigendum to 'A review of the basics of mitochondrial bioenergetics, metabolism, and related signaling pathways in cancer cells: Therapeutic targeting of tumor mitochondria with lipophilic cationic compounds' [REDOX 14C (2017) 316-327]. (Kalyanaraman B, Cheng G, Hardy M, Ouari O, Lopez M, Joseph J, Zielonka J, Dwinell MB) Redox Biol 2018 Jun;16:426-427 PMID: 29548725 PMCID: PMC5952812 SCOPUS ID: 2-s2.0-85043469209 03/20/2018       5 Citations
  • Teaching the basics of reactive oxygen species and their relevance to cancer biology: Mitochondrial reactive oxygen species detection, redox signaling, and targeted therapies. (Kalyanaraman B, Cheng G, Hardy M, Ouari O, Bennett B, Zielonka J) Redox Biol 2018 May;15:347-362 PMID: 29306792 PMCID: PMC5756055 SCOPUS ID: 2-s2.0-85039930921 01/08/2018       136 Citations
  • Detection and Characterization of Reactive Oxygen and Nitrogen Species in Biological Systems by Monitoring Species-Specific Products. (Hardy M, Zielonka J, Karoui H, Sikora A, Michalski R, Podsiadły R, Lopez M, Vasquez-Vivar J, Kalyanaraman B, Ouari O) Antioxid Redox Signal 2018 May 20;28(15):1416-1432 PMID: 29037049 PMCID: PMC5910052 SCOPUS ID: 2-s2.0-85045516114 10/19/2017       56 Citations
  • A review of the basics of mitochondrial bioenergetics, metabolism, and related signaling pathways in cancer cells: Therapeutic targeting of tumor mitochondria with lipophilic cationic compounds. (Kalyanaraman B, Cheng G, Hardy M, Ouari O, Lopez M, Joseph J, Zielonka J, Dwinell MB) Redox Biol 2018 Apr;14:316-327 PMID: 29017115 PMCID: PMC5633086 SCOPUS ID: 2-s2.0-85030681499 10/11/2017       138 Citations
  • Synthesis and properties of a series of β-cyclodextrin/nitrone spin traps for improved superoxide detection. (Poulhès F, Rizzato E, Bernasconi P, Rosas R, Viel S, Jicsinszky L, Rockenbauer A, Bardelang D, Siri D, Gaudel-Siri A, Karoui H, Hardy M, Ouari O) Org Biomol Chem 2017 Aug 02;15(30):6358-6366 PMID: 28715016 SCOPUS ID: 2-s2.0-85026838922 07/18/2017       6 Citations
  • Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases. (Zielonka J, Hardy M, Michalski R, Sikora A, Zielonka M, Cheng G, Ouari O, Podsiadły R, Kalyanaraman B) Cell Biochem Biophys 2017 Dec;75(3-4):335-349 PMID: 28660426 PMCID: PMC5693611 SCOPUS ID: 2-s2.0-85025160609 07/01/2017       18 Citations
  • Mitochondria-Targeted Triphenylphosphonium-Based Compounds: Syntheses, Mechanisms of Action, and Therapeutic and Diagnostic Applications. (Zielonka J, Joseph J, Sikora A, Hardy M, Ouari O, Vasquez-Vivar J, Cheng G, Lopez M, Kalyanaraman B) Chem Rev 2017 Aug 09;117(15):10043-10120 PMID: 28654243 PMCID: PMC5611849 SCOPUS ID: 2-s2.0-85027303022 06/28/2017       751 Citations
  • Modified Metformin as a More Potent Anticancer Drug: Mitochondrial Inhibition, Redox Signaling, Antiproliferative Effects and Future EPR Studies. (Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell MB) Cell Biochem Biophys 2017 Dec;75(3-4):311-317 PMID: 28429253 PMCID: PMC5680142 SCOPUS ID: 2-s2.0-85018513016 04/22/2017       18 Citations
  • Mitochondria-targeted metformins: anti-tumour and redox signalling mechanisms. (Kalyanaraman B, Cheng G, Hardy M, Ouari O, Sikora A, Zielonka J, Dwinell M) Interface Focus 2017 Apr 06;7(2):20160109 PMID: 28382202 PMCID: PMC5311906 SCOPUS ID: 2-s2.0-85013194895 04/07/2017       20 Citations
  • A Critical Review of Methodologies to Detect Reactive Oxygen and Nitrogen Species Stimulated by NADPH Oxidase Enzymes: Implications in Pesticide Toxicity. (Kalyanaraman B, Hardy M, Zielonka J) Curr Pharmacol Rep 2016 Aug;2(4):193-201 PMID: 27774407 PMCID: PMC5070669 SCOPUS ID: 2-s2.0-85012255350 10/25/2016       26 Citations
  • On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo - Identification of reaction intermediates and oxidant-specific minor products. (Zielonka J, Podsiadły R, Zielonka M, Hardy M, Kalyanaraman B) Free Radic Biol Med 2016 Oct;99:32-42 PMID: 27458121 PMCID: PMC5107150 SCOPUS ID: 2-s2.0-84979887634 10/23/2016       33 Citations
  • Recent developments in detection of superoxide radical anion and hydrogen peroxide: Opportunities, challenges, and implications in redox signaling. (Kalyanaraman B, Hardy M, Podsiadly R, Cheng G, Zielonka J) Arch Biochem Biophys 2017 Mar 01;617:38-47 PMID: 27590268 PMCID: PMC5318280 SCOPUS ID: 2-s2.0-84995422269 09/04/2016       75 Citations
  • Mitochondria-Targeted Analogues of Metformin Exhibit Enhanced Antiproliferative and Radiosensitizing Effects in Pancreatic Cancer Cells. (Cheng G, Zielonka J, Ouari O, Lopez M, McAllister D, Boyle K, Barrios CS, Weber JJ, Johnson BD, Hardy M, Dwinell MB, Kalyanaraman B) Cancer Res 2016 Jul 01;76(13):3904-15 PMID: 27216187 PMCID: PMC4930686 SCOPUS ID: 2-s2.0-84977588183 05/25/2016       131 Citations
  • Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit Peroxynitrite Formation. (Zielonka J, Zielonka M, VerPlank L, Cheng G, Hardy M, Ouari O, Ayhan MM, Podsiadły R, Sikora A, Lambeth JD, Kalyanaraman B) J Biol Chem 2016 Mar 25;291(13):7029-44 PMID: 26839313 PMCID: PMC4807286 SCOPUS ID: 2-s2.0-84964907042 02/04/2016       49 Citations
  • Correction to "Comprehensive Synthesis of Monohydroxy-Cucurbit[n]urils (n = 5, 6, 7, 8): High Purity and High Conversions". (Ayhan MM, Karoui H, Hardy M, Rockenbauer A, Charles L, Rosas R, Udachin K, Tordo P, Bardelang D, Ouari O) J Am Chem Soc 2016 Feb 17;138(6):2060 PMID: 26839971 SCOPUS ID: 2-s2.0-84958999162 02/04/2016       28 Citations
  • EPR Studies of the Binding Properties, Guest Dynamics, and Inner-Space Dimensions of a Water-Soluble Resorcinarene Capsule. (Ayhan MM, Casano G, Karoui H, Rockenbauer A, Monnier V, Hardy M, Tordo P, Bardelang D, Ouari O) Chemistry 2015 Nov 09;21(46):16404-10 PMID: 26403999 SCOPUS ID: 2-s2.0-84946091348 09/26/2015       12 Citations
  • Toward selective detection of reactive oxygen and nitrogen species with the use of fluorogenic probes--Limitations, progress, and perspectives. (Debowska K, Debski D, Hardy M, Jakubowska M, Kalyanaraman B, Marcinek A, Michalski R, Michalowski B, Ouari O, Sikora A, Smulik R, Zielonka J) Pharmacol Rep 2015 Aug;67(4):756-64 PMID: 26321278 PMCID: PMC5793900 SCOPUS ID: 2-s2.0-84940462191 09/01/2015       42 Citations
  • Comprehensive Synthesis of Monohydroxy-Cucurbit[n]urils (n = 5, 6, 7, 8): High Purity and High Conversions. (Ayhan MM, Karoui H, Hardy M, Rockenbauer A, Charles L, Rosas R, Udachin K, Tordo P, Bardelang D, Ouari O) J Am Chem Soc 2015 Aug 19;137(32):10238-45 PMID: 26197228 SCOPUS ID: 2-s2.0-84939857385 07/22/2015       83 Citations
  • Antiproliferative effects of mitochondria-targeted cationic antioxidants and analogs: Role of mitochondrial bioenergetics and energy-sensing mechanism. (Cheng G, Zielonka J, McAllister D, Hardy M, Ouari O, Joseph J, Dwinell MB, Kalyanaraman B) Cancer Lett 2015 Aug 28;365(1):96-106 PMID: 26004344 PMCID: PMC4476640 SCOPUS ID: 2-s2.0-84930823628 05/26/2015       47 Citations
  • Mitochondria-targeted spin traps: synthesis, superoxide spin trapping, and mitochondrial uptake. (Hardy M, Poulhés F, Rizzato E, Rockenbauer A, Banaszak K, Karoui H, Lopez M, Zielonka J, Vasquez-Vivar J, Sethumadhavan S, Kalyanaraman B, Tordo P, Ouari O) Chem Res Toxicol 2014 Jul 21;27(7):1155-65 PMID: 24890552 PMCID: PMC5452977 SCOPUS ID: 2-s2.0-84904659978 06/04/2014       23 Citations
  • Detection of superoxide production in stimulated and unstimulated living cells using new cyclic nitrone spin traps. (Abbas K, Hardy M, Poulhès F, Karoui H, Tordo P, Ouari O, Peyrot F) Free Radic Biol Med 2014 Jun;71:281-290 PMID: 24662195 SCOPUS ID: 2-s2.0-84898773391 03/26/2014       70 Citations
  • Synthesis and spin-trapping properties of a trifluoromethyl analogue of DMPO: 5-methyl-5-trifluoromethyl-1-pyrroline N-oxide (5-TFDMPO). (Karoui H, Nsanzumuhire C, Le Moigne F, Hardy M, Siri D, Derat E, Rockenbauer A, Ouari O, Tordo P) Chemistry 2014 Apr 01;20(14):4064-71 PMID: 24590621 SCOPUS ID: 2-s2.0-84897917219 03/05/2014       13 Citations
  • Metabolic stability of superoxide adducts derived from newly developed cyclic nitrone spin traps. (Bézière N, Hardy M, Poulhès F, Karoui H, Tordo P, Ouari O, Frapart YM, Rockenbauer A, Boucher JL, Mansuy D, Peyrot F) Free Radic Biol Med 2014 Feb;67:150-8 PMID: 24161442 SCOPUS ID: 2-s2.0-84888126364 10/29/2013       29 Citations
  • HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes--the ultimate approach for intra- and extracellular superoxide detection. (Kalyanaraman B, Dranka BP, Hardy M, Michalski R, Zielonka J) Biochim Biophys Acta 2014 Feb;1840(2):739-44 PMID: 23668959 PMCID: PMC3858408 SCOPUS ID: 2-s2.0-84890117297 05/15/2013       86 Citations
  • Hydropropidine: a novel, cell-impermeant fluorogenic probe for detecting extracellular superoxide. (Michalski R, Zielonka J, Hardy M, Joseph J, Kalyanaraman B) Free Radic Biol Med 2013 Jan;54:135-47 PMID: 23051008 PMCID: PMC3711142 SCOPUS ID: 2-s2.0-84871920872 10/12/2012       39 Citations
  • Boronate probes as diagnostic tools for real time monitoring of peroxynitrite and hydroperoxides. (Zielonka J, Sikora A, Hardy M, Joseph J, Dranka BP, Kalyanaraman B) Chem Res Toxicol 2012 Sep 17;25(9):1793-9 PMID: 22731669 PMCID: PMC3501381 SCOPUS ID: 2-s2.0-84866386085 06/27/2012       173 Citations
  • Global profiling of reactive oxygen and nitrogen species in biological systems: high-throughput real-time analyses. (Zielonka J, Zielonka M, Sikora A, Adamus J, Joseph J, Hardy M, Ouari O, Dranka BP, Kalyanaraman B) J Biol Chem 2012 Jan 27;287(5):2984-95 PMID: 22139901 PMCID: PMC3270955 SCOPUS ID: 2-s2.0-84856248241 12/06/2011       141 Citations
  • A (99m)Tc-labeled triphenylphosphonium derivative for the early detection of breast tumors. (Li Z, Lopez M, Hardy M, McAllister DM, Kalyanaraman B, Zhao M) Cancer Biother Radiopharm 2009 Oct;24(5):579-87 PMID: 19877888 PMCID: PMC2883499 SCOPUS ID: 2-s2.0-70449135470 11/03/2009       20 Citations
  • Improving the trapping of superoxide radical with a beta-cyclodextrin- 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO) conjugate. (Hardy M, Bardelang D, Karoui H, Rockenbauer A, Finet JP, Jicsinszky L, Rosas R, Ouari O, Tordo P) Chemistry 2009 Oct 26;15(42):11114-8 PMID: 19760720 SCOPUS ID: 2-s2.0-70350497338 09/18/2009       38 Citations
  • HPLC study of oxidation products of hydroethidine in chemical and biological systems: ramifications in superoxide measurements. (Zielonka J, Hardy M, Kalyanaraman B) Free Radic Biol Med 2009 Feb 01;46(3):329-38 PMID: 19026738 PMCID: PMC3375818 SCOPUS ID: 2-s2.0-58149168842 11/26/2008       126 Citations
  • Cytochrome c-mediated oxidation of hydroethidine and mito-hydroethidine in mitochondria: identification of homo- and heterodimers. (Zielonka J, Srinivasan S, Hardy M, Ouari O, Lopez M, Vasquez-Vivar J, Avadhani NG, Kalyanaraman B) Free Radic Biol Med 2008 Mar 01;44(5):835-46 PMID: 18155177 PMCID: PMC2692199 SCOPUS ID: 2-s2.0-39149083084 12/25/2007       87 Citations
  • Design of new derivatives of nitrone DEPMPO functionalized at C-4 for further specific applications in superoxide radical detection. (Chalier F, Hardy M, Ouari O, Rockenbauer A, Tordo P) J Org Chem 2007 Oct 12;72(21):7886-92 PMID: 17880238 SCOPUS ID: 2-s2.0-35348820519 09/21/2007       18 Citations
  • Detection, characterization, and decay kinetics of ROS and thiyl adducts of mito-DEPMPO spin trap. (Hardy M, Rockenbauer A, Vásquez-Vivar J, Felix C, Lopez M, Srinivasan S, Avadhani N, Tordo P, Kalyanaraman B) Chem Res Toxicol 2007 Jul;20(7):1053-60 PMID: 17559235 PMCID: PMC4455887 SCOPUS ID: 2-s2.0-34547615931 06/15/2007       58 Citations
  • Mito-DEPMPO synthesized from a novel NH2-reactive DEPMPO spin trap: a new and improved trap for the detection of superoxide. (Hardy M, Chalier F, Ouari O, Finet JP, Rockenbauer A, Kalyanaraman B, Tordo P) Chem Commun (Camb) 2007 Mar 14(10):1083-5 PMID: 17325813 SCOPUS ID: 2-s2.0-33847289280 02/28/2007       50 Citations
  • Synthesis and spin-trapping behavior of 5-ChEPMPO, a cholesteryl ester analogue of the spin trap DEPMPO. (Hardy M, Ouari O, Charles L, Finet JP, Iacazio G, Monnier V, Rockenbauer A, Tordo P) J Org Chem 2005 Dec 09;70(25):10426-33 PMID: 16323853 SCOPUS ID: 2-s2.0-28744446638 12/06/2005       26 Citations
  • Diastereoselective synthesis and ESR study of 4-phenylDEPMPO spin traps. (Hardy M, Chalier F, Finet JP, Rockenbauer A, Tordo P) J Org Chem 2005 Mar 18;70(6):2135-42 PMID: 15760197 SCOPUS ID: 2-s2.0-15444376393 03/12/2005       14 Citations
  • Last update: 07/05/2022