Recent Developments in the Probes and Assays for Measurement of the Activity of NADPH Oxidases. Cell Biochem Biophys 2017 Dec;75(3-4):335-349
Date
07/01/2017Pubmed ID
28660426Pubmed Central ID
PMC5693611DOI
10.1007/s12013-017-0813-6Scopus ID
2-s2.0-85025160609 (requires institutional sign-in at Scopus site) 22 CitationsAbstract
NADPH oxidases are a family of enzymes capable of transferring electrons from NADPH to molecular oxygen. A major function of NADPH oxidases is the activation of molecular oxygen into reactive oxygen species. Increased activity of NADPH oxidases has been implicated in various pathologies, including cardiovascular disease, neurological dysfunction, and cancer. Thus, NADPH oxidases have been identified as a viable target for the development of novel therapeutics exhibiting inhibitory effects on NADPH oxidases. Here, we describe the development of new assays for measuring the activity of NADPH oxidases enabling the high-throughput screening for NADPH oxidase inhibitors.
Author List
Zielonka J, Hardy M, Michalski R, Sikora A, Zielonka M, Cheng G, Ouari O, Podsiadły R, Kalyanaraman BAuthors
Gang Cheng PhD Assistant Professor in the Biophysics department at Medical College of WisconsinMicael Joel Hardy PhD Visiting Assistant Professor in the Biophysics department at Medical College of Wisconsin
Balaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
Jacek M. Zielonka PhD Assistant Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Cardiovascular DiseasesChromatography, High Pressure Liquid
Electron Spin Resonance Spectroscopy
Fluorescent Dyes
High-Throughput Screening Assays
Humans
Hydrogen Peroxide
Luminescent Measurements
NADPH Oxidases
Neoplasms
Oxidation-Reduction
Spectrophotometry
Superoxides
