Toward selective detection of reactive oxygen and nitrogen species with the use of fluorogenic probes--Limitations, progress, and perspectives. Pharmacol Rep 2015 Aug;67(4):756-64
Date
09/01/2015Pubmed ID
26321278Pubmed Central ID
PMC5793900DOI
10.1016/j.pharep.2015.03.016Scopus ID
2-s2.0-84940462191 (requires institutional sign-in at Scopus site) 51 CitationsAbstract
Over the last 40 years, there has been tremendous progress in understanding the biological reactions of reactive oxygen species (ROS) and reactive nitrogen species (RNS). It is widely accepted that the generation of ROS and RNS is involved in physiological and pathophysiological processes. To understand the role of ROS and RNS in a variety of pathologies, the specific detection of ROS and RNS is fundamental. Unfortunately, the intracellular detection and quantitation of ROS and RNS remains a challenge. In this short review, we have focused on the mechanistic and quantitative aspects of their detection with the use of selected fluorogenic probes. The challenges, limitations and perspectives of these methods are discussed.
Author List
Debowska K, Debski D, Hardy M, Jakubowska M, Kalyanaraman B, Marcinek A, Michalski R, Michalowski B, Ouari O, Sikora A, Smulik R, Zielonka JAuthors
Micael Joel Hardy PhD Visiting Assistant Professor in the Biophysics department at Medical College of WisconsinBalaraman Kalyanaraman PhD Professor in the Biophysics department at Medical College of Wisconsin
Jacek M. Zielonka PhD Assistant Professor in the Biophysics department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsFluorescent Dyes
Humans
Reactive Nitrogen Species
Reactive Oxygen Species
