Medical College of Wisconsin
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Brian Smith PhD

Brian Smith PhD

Assistant Professor

Institution: Medical College of Wisconsin
Department: Biochemistry

Member of the Cancer Center
Member of the Cardiovascular Center


Research Experience

  • Alkylating Agents
  • Amino Acids, Peptides, and Proteins
  • Binding Sites
  • Biochemistry
  • Catalysis
  • Deuterium Exchange Measurement
  • Enzyme Activators
  • Enzyme Inhibitors
  • Enzymes and Coenzymes
  • Histone Code
  • Histone Deacetylases
  • Mass Spectrometry
  • Molecular Probes
  • Molecular Structure
  • Nitric Oxide Donors
  • Nitric Oxide Synthase
  • Peptide Library
  • Peptides
  • Protein Binding
  • Proteins
  • Proteomics
  • Reducing Agents
  • Sirtuins
  • Structure-Activity Relationship
  • Substrate Specificity
  • Methodologies and Techniques

  • Biochemistry
  • Biological Assay
  • Chromatography, Affinity
  • Electrophoresis
  • Mass Spectrometry
  • Models, Chemical
  • Models, Molecular
  • Mutagenesis
  • Nuclear Magnetic Resonance, Biomolecular
  • Peptide Library
  • Protein Array Analysis
  • Proteomics
  • chemical synthesis
  • enzymology
  • MCW Program / Core Facilities

  • Redox Biology
  • Biochemistry
  • Publications

  • Mechanism of Sirt1 NAD+-dependent Protein Deacetylase Inhibition by Cysteine S-Nitrosation. (Kalous KS, Wynia-Smith SL, Olp MD, Smith BC) J Biol Chem 2016 Dec 02;291(49):25398-25410 PMID: 27756843 PMCID: PMC5207242 10/21/2016
  • Nitrosothiol formation and S-nitrosation signaling through nitric oxide synthases. (Wynia-Smith SL, Smith BC) Nitric Oxide 2017 Feb 28;63:52-60 PMID: 27720836 10/11/2016
  • Chemoproteomic Strategy to Quantitatively Monitor Transnitrosation Uncovers Functionally Relevant S-Nitrosation Sites on Cathepsin D and HADH2. (Zhou Y, Wynia-Smith SL, Couvertier SM, Kalous KS, Marletta MA, Smith BC, Weerapana E) Cell Chem Biol 2016 Jun 23;23(6):727-37 PMID: 27291402 PMCID: PMC4920707 06/14/2016
  • Reply: To PMID 25479138. (Shteyer E, Smith B) Gastroenterology 2015 Jul;149(1):261-2 PMID: 26025311 05/31/2015
  • Nitric Oxide Mediates Biofilm Formation and Symbiosis in Silicibacter sp. Strain TrichCH4B. (Rao M, Smith BC, Marletta MA) MBio 2015 May 05;6(3):e00206-15 PMID: 25944856 PMCID: PMC4436077 05/07/2015
  • Truncating mutation in the nitric oxide synthase 1 gene is associated with infantile achalasia. (Shteyer E, Edvardson S, Wynia-Smith SL, Pierri CL, Zangen T, Hashavya S, Begin M, Yaacov B, Cinamon Y, Koplewitz BZ, Vromen A, Elpeleg O, Smith BC) Gastroenterology 2015 Mar;148(3):533-536.e4 PMID: 25479138 12/06/2014
  • Nitric oxide signaling (Surmeli NB, Underbakke ES, Smith BC, Wynia-Smith SL, Marletta MA) Comprehensive Inorganic Chemistry Vol. 3, 241-262 10/29/2014
  • Molecular architecture of mammalian nitric oxide synthases. (Campbell MG, Smith BC, Potter CS, Carragher B, Marletta MA) Proc Natl Acad Sci U S A 2014 Sep 02;111(35):E3614-23 PMID: 25125509 PMCID: PMC4156747 08/16/2014
  • Nitric oxide synthase domain interfaces regulate electron transfer and calmodulin activation. (Smith BC, Underbakke ES, Kulp DW, Schief WR, Marletta MA) Proc Natl Acad Sci U S A 2013 Sep 17;110(38):E3577-86 PMID: 24003111 PMCID: PMC3780838 09/05/2013
  • Mechanisms of S-nitrosothiol formation and selectivity in nitric oxide signaling. (Smith BC, Marletta MA) Curr Opin Chem Biol 2012 Dec;16(5-6):498-506 PMID: 23127359 PMCID: PMC3518608 11/07/2012
  • Mechanism and kinetics of inducible nitric oxide synthase auto-S-nitrosation and inactivation. (Smith BC, Fernhoff NB, Marletta MA) Biochemistry 2012 Feb 07;51(5):1028-40 PMID: 22242685 PMCID: PMC3277664 01/17/2012
  • Structural and kinetic isotope effect studies of nicotinamidase (Pnc1) from Saccharomyces cerevisiae. (Smith BC, Anderson MA, Hoadley KA, Keck JL, Cleland WW, Denu JM) Biochemistry 2012 Jan 10;51(1):243-56 PMID: 22229411 PMCID: PMC3257521 01/11/2012
  • Hydrolysis of O-acetyl-ADP-ribose isomers by ADP-ribosylhydrolase 3. (Kasamatsu A, Nakao M, Smith BC, Comstock LR, Ono T, Kato J, Denu JM, Moss J) J Biol Chem 2011 Jun 17;286(24):21110-7 PMID: 21498885 PMCID: PMC3122172 04/19/2011
  • Sirt3 promotes the urea cycle and fatty acid oxidation during dietary restriction. (Hallows WC, Yu W, Smith BC, Devries MK, Ellinger JJ, Someya S, Shortreed MR, Prolla T, Markley JL, Smith LM, Zhao S, Guan KL, Denu JM) Mol Cell 2011 Jan 21;41(2):139-49 PMID: 21255725 PMCID: PMC3101115 01/25/2011
  • SIRT3 substrate specificity determined by peptide arrays and machine learning. (Smith BC, Settles B, Hallows WC, Craven MW, Denu JM) ACS Chem Biol 2011 Feb 18;6(2):146-57 PMID: 20945913 PMCID: PMC3042044 10/16/2010
  • A continuous microplate assay for sirtuins and nicotinamide-producing enzymes. (Smith BC, Hallows WC, Denu JM) Anal Biochem 2009 Nov 01;394(1):101-9 PMID: 19615966 PMCID: PMC2752052 07/21/2009
  • Ure(k)a! Sirtuins Regulate Mitochondria. (Hallows WC, Smith BC, Lee S, Denu JM) Cell 2009 May 01;137(3):404-6 PMID: 19410538 PMCID: PMC3310393 05/05/2009
  • Mechanisms and molecular probes of sirtuins. (Smith BC, Hallows WC, Denu JM) Chem Biol 2008 Oct 20;15(10):1002-13 PMID: 18940661 PMCID: PMC2626554 10/23/2008
  • Chemical mechanisms of histone lysine and arginine modifications. (Smith BC, Denu JM) Biochim Biophys Acta 2009 Jan;1789(1):45-57 PMID: 18603028 PMCID: PMC2642981 07/08/2008
  • Mechanism-based inhibition of Sir2 deacetylases by thioacetyl-lysine peptide. (Smith BC, Denu JM) Biochemistry 2007 Dec 18;46(50):14478-86 PMID: 18027980 11/22/2007
  • Acetyl-lysine analog peptides as mechanistic probes of protein deacetylases. (Smith BC, Denu JM) J Biol Chem 2007 Dec 21;282(51):37256-65 PMID: 17951578 10/24/2007
  • Linking SIRT2 to Parkinson's disease. (Garske AL, Smith BC, Denu JM) ACS Chem Biol 2007 Aug 17;2(8):529-32 PMID: 17708669 08/22/2007
  • Sir2 deacetylases exhibit nucleophilic participation of acetyl-lysine in NAD+ cleavage. (Smith BC, Denu JM) J Am Chem Soc 2007 May 09;129(18):5802-3 PMID: 17439123 PMCID: PMC2568996 04/19/2007
  • Sirtuins caught in the act. (Smith BC, Denu JM) Structure 2006 Aug;14(8):1207-8 PMID: 16905094 08/15/2006
  • Sir2 protein deacetylases: evidence for chemical intermediates and functions of a conserved histidine. (Smith BC, Denu JM) Biochemistry 2006 Jan 10;45(1):272-82 PMID: 16388603 PMCID: PMC2519119 01/04/2006
  • Small molecule regulation of Sir2 protein deacetylases. (Grubisha O, Smith BC, Denu JM) FEBS J 2005 Sep;272(18):4607-16 PMID: 16156783 09/15/2005
  • Mechanism of human SIRT1 activation by resveratrol. (Borra MT, Smith BC, Denu JM) J Biol Chem 2005 Apr 29;280(17):17187-95 PMID: 15749705 03/08/2005
  • Coenzyme specificity of Sir2 protein deacetylases: implications for physiological regulation. (Schmidt MT, Smith BC, Jackson MD, Denu JM) J Biol Chem 2004 Sep 17;279(38):40122-9 PMID: 15269219 07/23/2004
  • Last update: 09/11/2017
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