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Sally S. Twining PhD

Sally S. Twining PhD

Assistant Dean, Professor

Institution: Medical College of Wisconsin
Department: Biochemistry

Member of the Cancer Center
Member of the Neuroscience Research Center


Research Experience

  • Breast Neoplasms
  • Corneal Diseases
  • Corneal Neovascularization
  • Corneal Opacity
  • Enzyme Activation
  • Keratitis
  • Keratoconus
  • Pseudomonas aeruginosa
  • Receptors, Proteinase-Activated
  • Serpins
  • Thrombin
  • Maspin
  • Leadership Positions

  • Sr Vice President Faculty Council: 2007-2008
  • President Faculty Council: 2008-2009
  • Vice President: 2006-2007
  • Chair Women's Faculty Council: 2005-2006
  • Chair M1-2 Subcommittee Curriculum and Evaluation Committee: 2002-2005
  • Publications

  • Expression of insulin-like growth factor 2 receptor in corneal keratocytes during differentiation and in response to wound healing. (Bohnsack RN, Warejcka DJ, Wang L, Gillespie SR, Bernstein AM, Twining SS, Dahms NM) Invest Ophthalmol Vis Sci 2014 Oct 30;55(12):7697-708 PMID: 25358730 PMCID: PMC4249949 11/02/2014       7 Citations
  • Degradation of internalized αvβ5 integrin is controlled by uPAR bound uPA: effect on β1 integrin activity and α-SMA stress fiber assembly. (Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM) PLoS One 2012;7(3):e33915 PMID: 22470492 PMCID: PMC3309951 04/04/2012       13 Citations
  • Latency-associated peptide of transforming growth factor-β1 is not subject to physiological mannose phosphorylation. (Barnes J, Warejcka D, Simpliciano J, Twining S, Steet R) J Biol Chem 2012 Mar 02;287(10):7526-34 PMID: 22262853 PMCID: PMC3293579 01/21/2012       5 Citations
  • Maspin increases extracellular plasminogen activator activity associated with corneal fibroblasts and myofibroblasts. (Warejcka DJ, Narayan M, Twining SS) Exp Eye Res 2011 Nov;93(5):618-27 PMID: 21810423 PMCID: PMC3221939 08/04/2011       6 Citations
  • Maspin, the molecular bridge between the plasminogen activator system and beta1 integrin that facilitates cell adhesion. (Endsley MP, Hu Y, Deng Y, He X, Warejcka DJ, Twining SS, Gonias SL, Zhang M) J Biol Chem 2011 Jul 15;286(28):24599-607 PMID: 21606500 PMCID: PMC3137035 05/25/2011       27 Citations
  • Identification of phosphorylation sites on extracellular corneal epithelial cell maspin. (Narayan M, Mirza SP, Twining SS) Proteomics 2011 Apr;11(8):1382-90 PMID: 21365746 PMCID: PMC3098045 03/03/2011       10 Citations
  • PepD participates in the mycobacterial stress response mediated through MprAB and SigE. (White MJ, He H, Penoske RM, Twining SS, Zahrt TC) J Bacteriol 2010 Mar;192(6):1498-510 PMID: 20061478 PMCID: PMC2832534 01/12/2010       34 Citations
  • Residues essential for plasminogen binding by the cation-independent mannose 6-phosphate receptor. (Bohnsack RN, Patel M, Olson LJ, Twining SS, Dahms NM) Biochemistry 2010 Jan 26;49(3):635-44 PMID: 20028034 PMCID: PMC2814163 12/24/2009       8 Citations
  • Focus on molecules: maspin. (Narayan M, Twining S) Exp Eye Res 2010 Jan;90(1):2-3 PMID: 19615364 PMCID: PMC2789168 07/21/2009       10 Citations
  • Epigenetic silencing of maspin expression occurs early in the conversion of keratocytes to fibroblasts. (Horswill MA, Narayan M, Warejcka DJ, Cirillo LA, Twining SS) Exp Eye Res 2008 Apr;86(4):586-600 PMID: 18291368 PMCID: PMC2374753 02/23/2008       20 Citations
  • Urokinase receptor cleavage: a crucial step in fibroblast-to-myofibroblast differentiation. (Bernstein AM, Twining SS, Warejcka DJ, Tall E, Masur SK) Mol Biol Cell 2007 Jul;18(7):2716-27 PMID: 17507651 PMCID: PMC1924808 05/18/2007       57 Citations
  • Corneal activation of prothrombin to form thrombin, independent of vascular injury. (Ayala A, Warejcka DJ, Olague-Marchan M, Twining SS) Invest Ophthalmol Vis Sci 2007 Jan;48(1):134-43 PMID: 17197526 01/02/2007       8 Citations
  • The fibrinolysis inhibitor alpha2-antiplasmin in the human cornea. (Ayala A, Warejecka DJ, Vaughan KA, Twining SS, Yue BY) Curr Eye Res 2005 Dec;30(12):1097-103 PMID: 16354623 12/16/2005       4 Citations
  • Differential conversion of plasminogen to angiostatin by human corneal cell populations. (Warejcka DJ, Vaughan KA, Bernstein AM, Twining SS) Mol Vis 2005 Oct 20;11:859-68 PMID: 16270025 11/05/2005       9 Citations
  • Identification of a novel secreted protease from Pseudomonas aeruginosa that causes corneal erosions. (Marquart ME, Caballero AR, Chomnawang M, Thibodeaux BA, Twining SS, O'Callaghan RJ) Invest Ophthalmol Vis Sci 2005 Oct;46(10):3761-8 PMID: 16186360 09/28/2005       51 Citations
  • Specific conformational changes of plasminogen induced by chloride ions, 6-aminohexanoic acid and benzamidine, but not the overall openness of plasminogen regulate, production of biologically active angiostatins. (Warejcka DJ, Twining SS) Biochem J 2005 Dec 15;392(Pt 3):703-12 PMID: 16097950 PMCID: PMC1316312 08/16/2005       8 Citations
  • The effects of sub-solar levels of UV-A and UV-B on rabbit corneal and lens epithelial cells. (Rogers CS, Chan LM, Sims YS, Byrd KD, Hinton DL, Twining SS) Exp Eye Res 2004 May;78(5):1007-14 PMID: 15051481 03/31/2004       46 Citations
  • Mutation of lasA and lasB reduces Pseudomonas aeruginosa invasion of epithelial cells. (Cowell BA, Twining SS, Hobden JA, Kwong MS, Fleiszig SM) Microbiology 2003 Aug;149(Pt 8):2291-9 PMID: 12904569 08/09/2003       63 Citations
  • Sufficiency of the reactive site loop of maspin for induction of cell-matrix adhesion and inhibition of cell invasion. Conversion of ovalbumin to a maspin-like molecule. (Ngamkitidechakul C, Warejcka DJ, Burke JM, O'Brien WJ, Twining SS) J Biol Chem 2003 Aug 22;278(34):31796-806 PMID: 12799381 06/12/2003       64 Citations
  • Buffered non-fermenter system for lab-scale production of secreted recombinant His-tagged proteins in Saccharomyces cerevisiae. (Ngamkitidechakul C, Twining SS) Biotechniques 2002 Dec;33(6):1296-300 PMID: 12503315 12/31/2002       10 Citations
  • Maspin: synthesis by human cornea and regulation of in vitro stromal cell adhesion to extracellular matrix. (Ngamkitidechakul C, Burke JM, O'Brien WJ, Twining SS) Invest Ophthalmol Vis Sci 2001 Dec;42(13):3135-41 PMID: 11726614 12/01/2001    
  • Functional characterization of arginine 30, lysine 40, and arginine 62 in Tn5 transposase. (Twining SS, Goryshin IY, Bhasin A, Reznikoff WS) J Biol Chem 2001 Jun 22;276(25):23135-43 PMID: 11283001 04/03/2001       14 Citations
  • The serpin alpha1-proteinase inhibitor is a critical substrate for gelatinase B/MMP-9 in vivo. (Liu Z, Zhou X, Shapiro SD, Shipley JM, Twining SS, Diaz LA, Senior RM, Werb Z) Cell 2000 Sep 01;102(5):647-55 PMID: 11007483 09/28/2000       301 Citations
  • A disease-associated glycine substitution in BP180 (type XVII collagen) leads to a local destabilization of the major collagen triple helix. (Olague-Marchan M, Twining SS, Hacker MK, McGrath JA, Diaz LA, Giudice GJ) Matrix Biol 2000 Jul;19(3):223-33 PMID: 10936447 08/11/2000       12 Citations
  • Blood glucose laboratory for first-year medical students: an interdisciplinary model for nutrition-focused diabetes management. (Reiter SA, McGill CR, Lawrence SL, Twining SS) J Am Diet Assoc 2000 May;100(5):570-2 PMID: 10812384 05/17/2000       2 Citations
  • A critical role for neutrophil elastase in experimental bullous pemphigoid. (Liu Z, Shapiro SD, Zhou X, Twining SS, Senior RM, Giudice GJ, Fairley JA, Diaz LA) J Clin Invest 2000 Jan;105(1):113-23 PMID: 10619867 PMCID: PMC382581 01/05/2000       129 Citations
  • Tn5: A molecular window on transposition. (Reznikoff WS, Bhasin A, Davies DR, Goryshin IY, Mahnke LA, Naumann T, Rayment I, Steiniger-White M, Twining SS) Biochem Biophys Res Commun 1999 Dec 29;266(3):729-34 PMID: 10603311 12/22/1999       39 Citations
  • Extrahepatic synthesis of plasminogen in the human cornea is up-regulated by interleukins-1alpha and -1beta. (Twining SS, Wilson PM, Ngamkitidechakul C) Biochem J 1999 May 01;339 ( Pt 3):705-12 PMID: 10215610 PMCID: PMC1220207 04/24/1999       45 Citations
  • In vitro cellular toxicity predicts Pseudomonas aeruginosa virulence in lung infections. (Sawa T, Ohara M, Kurahashi K, Twining SS, Frank DW, Doroques DB, Long T, Gropper MA, Wiener-Kronish JP) Infect Immun 1998 Jul;66(7):3242-9 PMID: 9632591 PMCID: PMC108338 06/25/1998       91 Citations
  • Local control of alpha1-proteinase inhibitor levels: regulation of alpha1-proteinase inhibitor in the human cornea by growth factors and cytokines. (Bosković G, Twining SS) Biochim Biophys Acta 1998 May 27;1403(1):37-46 PMID: 9622589 06/12/1998       40 Citations
  • Expression of degradative enzymes and protease inhibitors in corneas with keratoconus. (Zhou L, Sawaguchi S, Twining SS, Sugar J, Feder RS, Yue BY) Invest Ophthalmol Vis Sci 1998 Jun;39(7):1117-24 PMID: 9620070 06/10/1998       167 Citations
  • Involvement of Sp1 elements in the promoter activity of the alpha1-proteinase inhibitor gene. (Li Y, Zhou L, Twining SS, Sugar J, Yue BY) J Biol Chem 1998 Apr 17;273(16):9959-65 PMID: 9545340 05/23/1998       33 Citations
  • Vitamin A deficiency alters rat neutrophil function. (Twining SS, Schulte DP, Wilson PM, Fish BL, Moulder JE) J Nutr 1997 Apr;127(4):558-65 PMID: 9109605 04/01/1997       50 Citations
  • Retinol and retinaldehyde specifically increase alpha1-proteinase inhibitor in the human cornea. (Bosković G, Twining SS) Biochem J 1997 Mar 15;322 ( Pt 3):751-6 PMID: 9148745 PMCID: PMC1218251 03/15/1997       14 Citations
  • Changes in rat corneal matrix metalloproteinases and serine proteinases under vitamin A deficiency. (Twining SS, Schulte DP, Zhou X, Wilson PM, Fish BL, Moulder JE) Curr Eye Res 1997 Feb;16(2):158-65 PMID: 9068947 02/01/1997       22 Citations
  • Cathepsin G, acid phosphatase, and alpha 1-proteinase inhibitor messenger RNA levels in keratoconus corneas. (Whitelock RB, Fukuchi T, Zhou L, Twining SS, Sugar J, Feder RS, Yue BY) Invest Ophthalmol Vis Sci 1997 Feb;38(2):529-34 PMID: 9040486 02/01/1997       55 Citations
  • Neutrophil cathepsin G is specifically decreased under vitamin A deficiency. (Twining SS, Schulte DP, Wilson PM, Zhou X, Fish BL, Moulder JE) Biochim Biophys Acta 1996 Nov 15;1317(2):112-8 PMID: 8950196 11/15/1996       13 Citations
  • Expression of wound healing and stress-related proteins in keratoconus corneas. (Zhou L, Yue BY, Twining SS, Sugar J, Feder RS) Curr Eye Res 1996 Nov;15(11):1124-31 PMID: 8950507 11/01/1996       53 Citations
  • Retinol is sequestered in the bone marrow of vitamin A-deficient rats. (Twining SS, Schulte DP, Wilson PM, Fish BL, Moulder JE) J Nutr 1996 Jun;126(6):1618-26 PMID: 8648436 06/01/1996       14 Citations
  • Effect of vitamin A deficiency on the early response to experimental Pseudomonas keratitis. (Twining SS, Zhou X, Schulte DP, Wilson PM, Fish B, Moulder J) Invest Ophthalmol Vis Sci 1996 Mar;37(4):511-22 PMID: 8595951 03/01/1996       23 Citations
  • A proposed sequence of reactions leading to collagen biosynthesis, fibrosis, and hypertension in alcoholics. (Brecher AS, Hinko A, Twining SS) J Investig Med 1996 Feb;44(2):36-41 PMID: 8689399 02/01/1996       11 Citations
  • The occupational and environmental health history: guidelines for the primary care nurse practitioner. (Twining S) Nurse Pract Forum 1995 Jun;6(2):64-71 PMID: 7620396 06/01/1995       1 Citations
  • Alpha 2-macroglobulin levels in normal human and keratoconus corneas. (Sawaguchi S, Twining SS, Yue BY, Chang SH, Zhou X, Loushin G, Sugar J, Feder RS) Invest Ophthalmol Vis Sci 1994 Nov;35(12):4008-14 PMID: 7525507 11/01/1994       67 Citations
  • Alpha 2-macroglobulin is present in and synthesized by the cornea. (Twining SS, Fukuchi T, Yue BY, Wilson PM, Zhou X, Loushin G) Invest Ophthalmol Vis Sci 1994 Jul;35(8):3226-33 PMID: 7519181 07/01/1994       32 Citations
  • Alpha 1-antichymotrypsin is present in and synthesized by the cornea. (Twining SS, Fukuchi T, Yue BY, Wilson PM, Zhou X) Curr Eye Res 1994 Jun;13(6):433-9 PMID: 7924407 06/01/1994       15 Citations
  • Corneal synthesis of alpha 1-proteinase inhibitor (alpha 1-antitrypsin). (Twining SS, Fukuchi T, Yue BY, Wilson PM, Boskovic G) Invest Ophthalmol Vis Sci 1994 Feb;35(2):458-62 PMID: 8112994 02/01/1994       49 Citations
  • Lysosomal enzyme and inhibitor levels in the human trabecular meshwork. (Sawaguchi S, Yue BY, Kawa JE, Chang IL, Twining SS, Meberg B) Invest Ophthalmol Vis Sci 1994 Jan;35(1):251-61 PMID: 8300353 01/01/1994       7 Citations
  • Regulation of proteolytic activity in tissues. (Twining SS) Crit Rev Biochem Mol Biol 1994;29(5):315-83 PMID: 7828434 01/01/1994       84 Citations
  • MMPs and proteinase inhibitors in the human aqueous humor. (Ando H, Twining SS, Yue BY, Zhou X, Fini ME, Kaiya T, Higginbotham EJ, Sugar J) Invest Ophthalmol Vis Sci 1993 Dec;34(13):3541-8 PMID: 7505006 12/01/1993       63 Citations
  • Effect of Pseudomonas aeruginosa elastase, alkaline protease, and exotoxin A on corneal proteinases and proteins. (Twining SS, Kirschner SE, Mahnke LA, Frank DW) Invest Ophthalmol Vis Sci 1993 Aug;34(9):2699-712 PMID: 8344792 08/01/1993       112 Citations
  • Metal sensitive protease(s) associated with corneal proteoglycans. (Twining SS, Wilson PM) Matrix Suppl 1992;1:91-2 PMID: 1480102 01/01/1992       1 Citations
  • Loss of stromal glycosaminoglycans during corneal edema. (Kangas TA, Edelhauser HF, Twining SS, O'Brien WJ) Invest Ophthalmol Vis Sci 1990 Oct;31(10):1994-2002 PMID: 2210995 10/01/1990       31 Citations
  • Alpha-1 proteinase inhibitor levels in keratoconus. (Sawaguchi S, Twining SS, Yue BY, Wilson PM, Sugar J, Chan SK) Exp Eye Res 1990 May;50(5):549-54 PMID: 2197100 05/01/1990       78 Citations
  • Immunotrophic methodology. (Nauss KM, Ross AC, Twining SS) Methods Enzymol 1990;190:259-73 PMID: 2087178 01/01/1990       1 Citations
  • Characterization of corneal proteoglycans under vitamin A deficiency. (Twining SS, Wilson PM, Hatchell DL) Biochim Biophys Acta 1989 Aug 18;992(2):181-90 PMID: 2758064 08/18/1989       4 Citations
  • Localization and quantification of alpha-1-proteinase inhibitor in the human cornea. (Twining SS, Everse SJ, Wilson PM, Yue BY, Chan SK) Curr Eye Res 1989 Apr;8(4):389-95 PMID: 2785899 04/01/1989       11 Citations
  • Regional comparisons of cathepsin D activity in bovine retinal pigment epithelium. (Burke JM, Twining SS) Invest Ophthalmol Vis Sci 1988 Dec;29(12):1789-93 PMID: 3192367 12/01/1988       14 Citations
  • Vitreous macrophage elicitation: generation of stimulants for pigment epithelium in vitro. (Burke JM, Twining SS) Invest Ophthalmol Vis Sci 1987 Jul;28(7):1100-7 PMID: 3596990 07/01/1987       20 Citations
  • Relationship between proteases and descemetocele formation in experimental Pseudomonas keratitis. (Twining SS, Davis SD, Hyndiuk RA) Curr Eye Res 1986 Jul;5(7):503-10 PMID: 3527568 07/01/1986       19 Citations
  • Role of leukocytes in ocular inflammation of tyrosinemia II. (Ripple RE, Lohr KM, Twining SS, Hyndiuk RA, Caya JG) Invest Ophthalmol Vis Sci 1986 Jun;27(6):926-31 PMID: 3519521 06/01/1986       7 Citations
  • The immune system in experimental Pseudomonas keratitis. Model and early effects. (Twining SS, Lohr KM, Moulder JE) Invest Ophthalmol Vis Sci 1986 Apr;27(4):507-15 PMID: 3082789 04/01/1986       28 Citations
  • Acid proteases and histologic correlations in experimental ulceration in vitamin A deficient rabbit corneas. (Twining SS, Hatchell DL, Hyndiuk RA, Nassif KF) Invest Ophthalmol Vis Sci 1985 Jan;26(1):31-44 PMID: 3881366 01/01/1985       15 Citations
  • Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes. (Twining SS) Anal Biochem 1984 Nov 15;143(1):30-4 PMID: 6442109 11/15/1984       459 Citations
  • Acid proteases of vitreal macrophages. (Twining S, Potts D, Burke J) Curr Eye Res 1984 Aug;3(8):1055-62 PMID: 6488857 08/01/1984       8 Citations
  • A pepsinogen from rainbow trout. (Twining SS, Alexander PA, Huibregtse K, Glick DM) Comp Biochem Physiol B 1983;75(1):109-12 PMID: 6406134 01/01/1983       44 Citations
  • A pepsinogen from dog stomach. (Twining SS, Huibregtse K, Glick DM) Comp Biochem Physiol B 1983;75(1):103-7 PMID: 6406133 01/01/1983       5 Citations
  • Genetic control of the immune response to myoglobin. VI. Inter-site influences in T-lymphocyte proliferative response from analysis of cross-reactions of ten myoglobins in terms of substitutions in the antigenic sites and in environmental residues of the sites. (Atassi MZ, Yokota S, Twining SS, Lehmann H, David CS) Mol Immunol 1981 Oct;18(10):945-8 PMID: 6977699 10/01/1981       19 Citations
  • Time-dependent study of the antibody response to sperm-whale myoglobin: recognition of the antigenic sites is unaltered over an extended period of immunization. (Twining SS, Lehmann H, Atassi MZ) Mol Immunol 1981 Jun;18(6):473-9 PMID: 6171719 06/01/1981       2 Citations
  • Genetic control of the immune response to myoglobin. IV. Mouse antibodies in outbred and congenic strains against sperm-whale myoglobin recognize the same antigenic sites that are recognized by antibodies raised in other species. (Twining SS, David CS, Atassi MZ) Mol Immunol 1981 May;18(5):447-50 PMID: 6171717 05/01/1981       51 Citations
  • Preparation and activation of a spin-labeled pepsinogen. (Twining SS, Sealy RC, Glick DM) Biochemistry 1981 Mar 03;20(5):1267-72 PMID: 6261787 03/03/1981       14 Citations
  • Genetic control of the immune response to myoglobin. V. Analysis of the cross-reactivity of 12 myoglobins with sperm-whale myoglobin antisera of inbred mouse strains in terms of substitutions in the antigenic sites and in the environmental residues of the sites. (Atassi MZ, Twining SS, Lehmann H, David CS) Immunol Commun 1981;10(4-5):359-65 PMID: 7333635 01/01/1981       3 Citations
  • The antibody response to myoglobin is independent of the immunized species. Analysis in terms of replacements in the antigenic sites and in environmental residues of the cross-reactions of fifteen myoglobins with sperm-whale myoglobin antisera raised in different species. (Twining SS, Lehmann H, Atassi MZ) Biochem J 1980 Dec 01;191(3):681-97 PMID: 6169338 PMCID: PMC1162269 12/01/1980       52 Citations
  • Genetic control of immune response to sperm whale myoglobin in mice. II. T lymphocyte proliferative response to the synthetic antigenic sites. (Okuda K, Twining SS, David CS, Atassi MZ) J Immunol 1979 Jul;123(1):182-8 PMID: 312873 07/01/1979       109 Citations
  • Use of immunoadsorbents for the study of antibody binding to sperm whale myoglobin and its synthetic antigenic sites. (Twining SS, Atassi MZ) J Immunol Methods 1979;30(2):139-51 PMID: 91647 01/01/1979       62 Citations
  • Genetic control of immune response to sperm whale myoglobin in mice. I. T lymphocyte proliferative response under H-2-linked Ir gene control. (Okuda K, Christadoss PR, Twining S, Atassi MZ, David CS) J Immunol 1978 Sep;121(3):866-8 PMID: 99478 09/01/1978       65 Citations
  • Antibody-combining sites can be mimicked synthetically. Surface-simulation synthesis of the immunoglobulin new combining site to the gamma-hydroxyl derivative of vitamin K1. (Twining SS, Atassi MZ) J Biol Chem 1978 Aug 10;253(15):5259-62 PMID: 670193 08/10/1978       14 Citations
  • Large scale separation of protease inhibitors from malignant human breast tissue. (Twining SS, Brecher AS) Mol Cell Biochem 1977 Dec 29;18(2-3):101-7 PMID: 75499 12/29/1977       6 Citations
  • Identification of alpha1-acid glycoprotein, alpha2-macroglobulin and antithrombin III as components of normal and malignant human tissues. (Twining SS, Brecher AS) Clin Chim Acta 1977 Feb 15;75(1):143-8 PMID: 66107 02/15/1977       12 Citations
  • Characterization of the interaction between chymotrypsin and heparin. (Twining SS, Brecher AS) Can J Biochem 1977 Feb;55(2):134-9 PMID: 14774 02/01/1977       1 Citations
  • Isolation and identification of alpha1-antitrypsin as a component of normal and malignant human breast and other tissues. (Twining SS, Brecher AS) Proc Soc Exp Biol Med 1975 Oct;150(1):98-103 PMID: 1081233 10/01/1975       8 Citations
  • Last update: 09/27/2015
    jenkins-FCD Prod-300 626508253d14e4184314fb9f66322a03a5906796