Cardiometabolic Effects of DOCA-Salt in Mice Depend on Ambient Temperature. Hypertension 2023 Sep;80(9):1871-1880
Date
07/20/2023Pubmed ID
37470185Pubmed Central ID
PMC10528934DOI
10.1161/HYPERTENSIONAHA.122.20415Scopus ID
2-s2.0-85168254483 (requires institutional sign-in at Scopus site)Abstract
BACKGROUND: Mice prefer warmer environments than humans. For this reason, behavioral and physiological thermoregulatory responses are engaged by mice in response to a standard room temperature of 22 to 24 °C. Autonomic mechanisms mediating thermoregulatory responses overlap with mechanisms activated in hypertension, and, therefore, we hypothesized that housing at thermoneutral temperatures (TNs; 30 °C) would modify the cardiometabolic effects of deoxycorticosterone acetate (DOCA)-salt in mice.
METHODS: The effects of DOCA-salt treatment upon ingestive behaviors, energy expenditure, blood pressure, heart rate (HR), and core temperature were assessed in C57BL/6J mice housed at room temperature or TN.
RESULTS: Housing at TN reduced food intake, energy expenditure, blood pressure, and HR and attenuated HR responses to acute autonomic blockade by chlorisondamine. At room temperature, DOCA-salt caused expected increases in fluid intake, sodium retention in osmotically inactive pools, blood pressure, core temperature, and also caused expected decreases in fat-free mass, total body water, and HR. At TN, the effects of DOCA-salt upon fluid intake, fat gains, hydration, and core temperature were exaggerated, but effects on energy expenditure and HR were blunted. Effects of DOCA-salt upon blood pressure were similar for 3 weeks and exaggerated by TN housing in the fourth week.
CONCLUSIONS: Ambient temperature robustly influences behavioral and physiological functions in mice, including metabolic and cardiovascular phenotype development in response to DOCA-salt treatment. Studying cardiometabolic responses of mice at optimal ambient temperatures promises to improve the translational relevance of rodent models.
Author List
Grobe CC, Reho JJ, Brown-Williams D, Ziegler AA, Mathieu NM, Lawton SBR, Fekete EM, Brozoski DT, Wackman KK, Burnett CML, Nakagawa P, Sigmund CD, Segar JL, Grobe JLAuthors
Eva M. Fekete PhD Research Scientist I in the Physiology department at Medical College of WisconsinJustin L. Grobe PhD Professor in the Physiology department at Medical College of Wisconsin
Pablo Nakagawa PhD Assistant Professor in the Physiology department at Medical College of Wisconsin
John J. Reho Research Scientist II in the Physiology department at Medical College of Wisconsin
Jeffrey L. Segar MD Professor in the Pediatrics department at Medical College of Wisconsin
Curt Sigmund PhD Chair, Professor in the Physiology department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
AnimalsBlood Pressure
Desoxycorticosterone
Desoxycorticosterone Acetate
Humans
Hypertension
Mice
Mice, Inbred C57BL
Temperature
