Faculty Collaboration Database

Differences between Burkitt's lymphomas and mouse plasmacytomas in the immunoglobulin heavy chain/c-myc recombinations that occur in their chromosomal translocations. Cancer Res 1995 Nov 01;55(21):5012-8

Date

11/01/1995

Pubmed ID

7585544

Scopus ID

2-s2.0-0028809713 (requires institutional sign-in at Scopus site)   30 Citations

Abstract

Burkitt's lymphomas (BLs) and mouse plasmacytomas (MPs) are characterized by chromosomal translocations juxtaposing the c-myc oncogene to one of the immunoglobulin loci. Here we describe illegitimate recombinations and the DNA sequence at the junction between c-myc and immunoglobulin heavy chain (IgH) regions in these B-cell lineage tumors. In 15 BLs and in 29 MPs, recombination products were amplified by PCR and sequenced. Molecular analysis of the IgH/c-myc junctions revealed the following characteristics: (a) rearrangements in BLs represent nearly reciprocal exchanges between c-myc and IgH switch sequences, whereas in MPs, several hundred bp of c-myc may be duplicated or deleted; (b) short homologies of up to 5 bp between immunoglobulin and c-myc sequences can be found at most junction sites in both tumors; and (c) pentameric sequences typically found in switch regions and V(D)J recognition motifs also occur frequently at the sites of recombination in c-myc.

Author List

Müller JR, Janz S, Potter M

Author

Siegfried Janz MD Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Base Sequence
Burkitt Lymphoma
DNA, Neoplasm
Gene Deletion
Genes, Immunoglobulin
Genes, myc
Humans
Mice
Molecular Sequence Data
Plasmacytoma
Recombination, Genetic
Sequence Homology, Nucleic Acid
Translocation, Genetic
Tumor Cells, Cultured