Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Blake W. Buchan PhD

Assistant Professor

Institution: Medical College of Wisconsin
Department: Pathology
Division: Administration


Publications

  • Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus. (Anderson NW, Buchan BW, Ledeboer NA) J Clin Microbiol 2014 Jan;52(1):2-8 PMID 24131689
  • Multicenter evaluation of the Verigene Clostridium difficile nucleic acid assay. (Carroll KC, Buchan BW, Tan S, Stamper PD, Riebe KM, Pancholi P, Kelly C, Rao A, Fader R, Cavagnolo R, Watson W, Goering RV, Trevino EA, Weissfeld AS, Ledeboer NA) J Clin Microbiol 2013 Dec;51(12):4120-5 PMID 24088862
  • Clinical evaluation of a real-time PCR assay for identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and shiga toxin-producing Escherichia coli isolates in stool specimens. (Buchan BW, Olson WJ, Pezewski M, Marcon MJ, Novicki T, Uphoff TS, Chandramohan L, Revell P, Ledeboer NA) J Clin Microbiol 2013 Dec;51(12):4001-7 PMID 24048539
  • Multiplex identification of gram-positive bacteria and resistance determinants directly from positive blood culture broths: evaluation of an automated microarray-based nucleic acid test. (Buchan BW, Ginocchio CC, Manii R, Cavagnolo R, Pancholi P, Swyers L, Thomson RB Jr, Anderson C, Kaul K, Ledeboer NA) PLoS Med 2013;10(7):e1001478 PMID 23843749
  • Multicenter clinical evaluation of VRESelect agar for identification of vancomycin-resistant Enterococcus faecalis and Enterococcus faecium. (Anderson NW, Buchan BW, Young CL, Newton DW, Brenke C, Lapsley L, Granato PA, Ledeboer NA) J Clin Microbiol 2013 Aug;51(8):2758-60 PMID 23761153
  • Multicenter clinical evaluation of the illumigene group A Streptococcus DNA amplification assay for detection of group A Streptococcus from pharyngeal swabs. (Anderson NW, Buchan BW, Mayne D, Mortensen JE, Mackey TL, Ledeboer NA) J Clin Microbiol 2013 May;51(5):1474-7 PMID 23447639
  • Advances in identification of clinical yeast isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. (Buchan BW, Ledeboer NA) J Clin Microbiol 2013 May;51(5):1359-66 PMID 23426924
  • Francisella tularensis live vaccine strain folate metabolism and pseudouridine synthase gene mutants modulate macrophage caspase-1 activation. (Ulland TK, Janowski AM, Buchan BW, Faron M, Cassel SL, Jones BD, Sutterwala FS) Infect Immun 2013 Jan;81(1):201-8 PMID 23115038
  • Multicenter clinical evaluation of the portrait toxigenic C. difficile assay for detection of toxigenic Clostridium difficile strains in clinical stool specimens. (Buchan BW, Mackey TL, Daly JA, Alger G, Denys GA, Peterson LR, Kehl SC, Ledeboer NA) J Clin Microbiol 2012 Dec;50(12):3932-6 PMID 23015667
  • Comparison of BD Phoenix and bioMérieux Vitek 2 automated systems for the detection of macrolide-lincosamide-streptogramin B resistance among clinical isolates of Staphylococcus. (Buchan BW, Anderson NW, Ledeboer NA) Diagn Microbiol Infect Dis 2012 Mar;72(3):291-4 PMID 22322000
  • Effects of solid-medium type on routine identification of bacterial isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. (Anderson NW, Buchan BW, Riebe KM, Parsons LN, Gnacinski S, Ledeboer NA) J Clin Microbiol 2012 Mar;50(3):1008-13 PMID 22162546
  • Comparison of the MALDI Biotyper system using Sepsityper specimen processing to routine microbiological methods for identification of bacteria from positive blood culture bottles. (Buchan BW, Riebe KM, Ledeboer NA) J Clin Microbiol 2012 Feb;50(2):346-52 PMID 22162549
  • Evaluation of a microarray-based genotyping assay for the rapid detection of cytochrome P450 2C19 *2 and *3 polymorphisms from whole blood using nanoparticle probes. (Buchan BW, Peterson JF, Cogbill CH, Anderson DK, Ledford JS, White MN, Quigley NB, Jannetto PJ, Ledeboer NA) Am J Clin Pathol 2011 Oct;136(4):604-8 PMID 21917683
  • Identification of two borderline oxacillin-resistant strains of Staphylococcus aureus from routine nares swab specimens by one of three chromogenic agars evaluated for the detection of MRSA. (Buchan BW, Ledeboer NA) Am J Clin Pathol 2010 Dec;134(6):921-7 PMID 21088156
  • Real-time detection of influenza a, influenza B, and respiratory syncytial virus a and B in respiratory specimens by use of nanoparticle probes. (Jannetto PJ, Buchan BW, Vaughan KA, Ledford JS, Anderson DK, Henley DC, Quigley NB, Ledeboer NA) J Clin Microbiol 2010 Nov;48(11):3997-4002 PMID 20826644
  • Cutting edge: mutation of Francisella tularensis mviN leads to increased macrophage absent in melanoma 2 inflammasome activation and a loss of virulence. (Ulland TK, Buchan BW, Ketterer MR, Fernandes-Alnemri T, Meyerholz DK, Apicella MA, Alnemri ES, Jones BD, Nauseef WM, Sutterwala FS) J Immunol 2010 Sep 1;185(5):2670-4 PMID 20679532
  • Multiple mechanisms of NADPH oxidase inhibition by type A and type B Francisella tularensis. (McCaffrey RL, Schwartz JT, Lindemann SR, Moreland JG, Buchan BW, Jones BD, Allen LA) J Leukoc Biol 2010 Oct;88(4):791-805 PMID 20610796
  • Identification of migR, a regulatory element of the Francisella tularensis live vaccine strain iglABCD virulence operon required for normal replication and trafficking in macrophages. (Buchan BW, McCaffrey RL, Lindemann SR, Allen LA, Jones BD) Infect Immun 2009 Jun;77(6):2517-29 PMID 19349423
  • Francisella tularensis genes required for inhibition of the neutrophil respiratory burst and intramacrophage growth identified by random transposon mutagenesis of strain LVS. (Schulert GS, McCaffrey RL, Buchan BW, Lindemann SR, Hollenback C, Jones BD, Allen LA) Infect Immun 2009 Apr;77(4):1324-36 PMID 19204089
  • Identification of differentially regulated francisella tularensis genes by use of a newly developed Tn5-based transposon delivery system. (Buchan BW, McLendon MK, Jones BD) Appl Environ Microbiol 2008 May;74(9):2637-45 PMID 18344342
  • Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium. (Sreenath HK, Bingman CA, Buchan BW, Seder KD, Burns BT, Geetha HV, Jeon WB, Vojtik FC, Aceti DJ, Frederick RO, Phillips GN Jr, Fox BG) Protein Expr Purif 2005 Apr;40(2):256-67 PMID 15766867
  • Grants and Awards

  • Reproducibility of the Xpert Flu+RSC Xpress Assay (#9306863 01/02/2015 - 01/02/2016) Sponsor: Cepheid
  • Illumigene HSV Beta and Clinical Trial (#9306612 06/20/2014 - 06/19/2015) Sponsor: Meridian Bioscience Inc
  • BioFire Lower Respiratory Tract Infection Assay Pilot Study (#9306695 09/17/2014 - 09/16/2015) Sponsor: BioFire Diagnostics, LLC
  • Great Basin Portrait STEC Clinical Trial (#9306839 12/18/2014 - 12/17/2016) Sponsor: Great Basin Corporation
  • Quidel Molecular Direct Group A + C/G Strep Assay (#9306272 08/01/2013 - 12/31/2013) Sponsor: QUIDEL CORP
  • NSPH RP Flex Clinical Trial (#9306611 04/15/2014 - 04/14/2015) Sponsor: Nanosphere, Inc.
  • Meridian TRU Staph/TRU MRSA (#9306156 06/11/2013 - 06/11/2015) Sponsor: Meridian Bioscience Inc
  • Quidel Solana GAS Clinical Trial (#9306827 12/01/2014 - 03/31/2015) Sponsor: QUIDEL CORP
  • Last update: 12/26/2013