Medical College of Wisconsin
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Blake W. Buchan PhD

Assistant Professor

Institution: Medical College of Wisconsin
Department: Pathology
Division: Administration


  • Identification of Gram-Negative Bacteria and Genetic Resistance Determinants from Positive Blood Culture Broths by Use of the Verigene Gram-Negative Blood Culture Multiplex Microarray-Based Molecular Assay. (Ledeboer NA, Lopansri BK, Dhiman N, Cavagnolo R, Carroll KC, Granato P, Thomson R Jr, Butler-Wu SM, Berger H, Samuel L, Pancholi P, Swyers L, Hansen GT, Tran NK, Polage CR, Thomson KS, Hanson ND, Winegar R, Buchan BW) J Clin Microbiol 2015 Aug;53(8):2460-72 PMID: 25994165 PMCID: PMC4508435 05/23/2015
  • Detection of Group A Streptococcus in Pharyngeal Swab Specimens by Use of the AmpliVue GAS Isothermal Helicase-Dependent Amplification Assay. (Faron ML, Ledeboer NA, Granato P, Daly JA, Pierce K, Pancholi P, Uphoff TS, Buchan BW) J Clin Microbiol 2015 Jul;53(7):2365-7 PMID: 25972419 PMCID: PMC4473221 05/15/2015
  • Multicenter evaluation of the BD max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes. (Harrington SM, Buchan BW, Doern C, Fader R, Ferraro MJ, Pillai DR, Rychert J, Doyle L, Lainesse A, Karchmer T, Mortensen JE) J Clin Microbiol 2015 May;53(5):1639-47 PMID: 25740779 PMCID: PMC4400754 03/06/2015
  • Comparison of the next-generation Xpert MRSA/SA BC assay and the GeneOhm StaphSR assay to routine culture for identification of Staphylococcus aureus and methicillin-resistant S. aureus in positive-blood-culture broths. (Buchan BW, Allen S, Burnham CA, McElvania TeKippe E, Davis T, Levi M, Mayne D, Pancholi P, Relich RF, Thomson R, Ledeboer NA) J Clin Microbiol 2015 Mar;53(3):804-9 PMID: 25540397 PMCID: PMC4390651 12/30/2014
  • Multicenter clinical evaluation of the Xpert GBS LB assay for detection of group B Streptococcus in prenatal screening specimens. (Buchan BW, Faron ML, Fuller D, Davis TE, Mayne D, Ledeboer NA) J Clin Microbiol 2015 Feb;53(2):443-8 PMID: 25411176 PMCID: PMC4298547 11/21/2014
  • Emerging technologies for the clinical microbiology laboratory. (Buchan BW, Ledeboer NA) Clin Microbiol Rev 2014 Oct;27(4):783-822 PMID: 25278575 PMCID: PMC4187641 10/04/2014
  • Multicenter evaluation of the Quidel Lyra Direct C. difficile nucleic acid amplification assay. (Beck ET, Buchan BW, Riebe KM, Alkins BR, Pancholi P, Granato PA, Ledeboer NA) J Clin Microbiol 2014 Jun;52(6):1998-2002 PMID: 24671790 PMCID: PMC4042815 03/29/2014
  • Clinical evaluation of the walk-away specimen processor and ESwab for recovery of Streptococcus agalactiae isolates in prenatal screening specimens. (Buchan BW, Olson WJ, Mackey TL, Ledeboer NA) J Clin Microbiol 2014 Jun;52(6):2166-8 PMID: 24622104 PMCID: PMC4042734 03/14/2014
  • Comparison of the BD MAX enteric bacterial panel to routine culture methods for detection of Campylobacter, enterohemorrhagic Escherichia coli (O157), Salmonella, and Shigella isolates in preserved stool specimens. (Anderson NW, Buchan BW, Ledeboer NA) J Clin Microbiol 2014 Apr;52(4):1222-4 PMID: 24430460 PMCID: PMC3993502 01/17/2014
  • Comparison of MALDI-TOF MS with HPLC and nucleic acid sequencing for the identification of Mycobacterium species in cultures using solid medium and broth. (Buchan BW, Riebe KM, Timke M, Kostrzewa M, Ledeboer NA) Am J Clin Pathol 2014 Jan;141(1):25-34 PMID: 24343734 12/18/2013
  • Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus. (Anderson NW, Buchan BW, Ledeboer NA) J Clin Microbiol 2014 Jan;52(1):2-8 PMID: 24131689 PMCID: PMC3911428 10/18/2013
  • Multicenter evaluation of the Verigene Clostridium difficile nucleic acid assay. (Carroll KC, Buchan BW, Tan S, Stamper PD, Riebe KM, Pancholi P, Kelly C, Rao A, Fader R, Cavagnolo R, Watson W, Goering RV, Trevino EA, Weissfeld AS, Ledeboer NA) J Clin Microbiol 2013 Dec;51(12):4120-5 PMID: 24088862 PMCID: PMC3838056 10/04/2013
  • Clinical evaluation of a real-time PCR assay for identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and shiga toxin-producing Escherichia coli isolates in stool specimens. (Buchan BW, Olson WJ, Pezewski M, Marcon MJ, Novicki T, Uphoff TS, Chandramohan L, Revell P, Ledeboer NA) J Clin Microbiol 2013 Dec;51(12):4001-7 PMID: 24048539 PMCID: PMC3838069 09/21/2013
  • Multiplex identification of gram-positive bacteria and resistance determinants directly from positive blood culture broths: evaluation of an automated microarray-based nucleic acid test. (Buchan BW, Ginocchio CC, Manii R, Cavagnolo R, Pancholi P, Swyers L, Thomson RB Jr, Anderson C, Kaul K, Ledeboer NA) PLoS Med 2013;10(7):e1001478 PMID: 23843749 PMCID: PMC3699453 07/12/2013
  • Multicenter clinical evaluation of VRESelect agar for identification of vancomycin-resistant Enterococcus faecalis and Enterococcus faecium. (Anderson NW, Buchan BW, Young CL, Newton DW, Brenke C, Lapsley L, Granato PA, Ledeboer NA) J Clin Microbiol 2013 Aug;51(8):2758-60 PMID: 23761153 PMCID: PMC3719601 06/14/2013
  • Multicenter clinical evaluation of the illumigene group A Streptococcus DNA amplification assay for detection of group A Streptococcus from pharyngeal swabs. (Anderson NW, Buchan BW, Mayne D, Mortensen JE, Mackey TL, Ledeboer NA) J Clin Microbiol 2013 May;51(5):1474-7 PMID: 23447639 PMCID: PMC3647941 03/01/2013
  • Advances in identification of clinical yeast isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. (Buchan BW, Ledeboer NA) J Clin Microbiol 2013 May;51(5):1359-66 PMID: 23426924 PMCID: PMC3647935 02/22/2013
  • Francisella tularensis live vaccine strain folate metabolism and pseudouridine synthase gene mutants modulate macrophage caspase-1 activation. (Ulland TK, Janowski AM, Buchan BW, Faron M, Cassel SL, Jones BD, Sutterwala FS) Infect Immun 2013 Jan;81(1):201-8 PMID: 23115038 PMCID: PMC3536133 11/02/2012
  • Multicenter clinical evaluation of the portrait toxigenic C. difficile assay for detection of toxigenic Clostridium difficile strains in clinical stool specimens. (Buchan BW, Mackey TL, Daly JA, Alger G, Denys GA, Peterson LR, Kehl SC, Ledeboer NA) J Clin Microbiol 2012 Dec;50(12):3932-6 PMID: 23015667 PMCID: PMC3502949 09/28/2012
  • Comparison of BD Phoenix and bioMérieux Vitek 2 automated systems for the detection of macrolide-lincosamide-streptogramin B resistance among clinical isolates of Staphylococcus. (Buchan BW, Anderson NW, Ledeboer NA) Diagn Microbiol Infect Dis 2012 Mar;72(3):291-4 PMID: 22322000 02/11/2012
  • Comparison of the MALDI Biotyper system using Sepsityper specimen processing to routine microbiological methods for identification of bacteria from positive blood culture bottles. (Buchan BW, Riebe KM, Ledeboer NA) J Clin Microbiol 2012 Feb;50(2):346-52 PMID: 22162549 PMCID: PMC3264150 12/14/2011
  • Effects of solid-medium type on routine identification of bacterial isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry. (Anderson NW, Buchan BW, Riebe KM, Parsons LN, Gnacinski S, Ledeboer NA) J Clin Microbiol 2012 Mar;50(3):1008-13 PMID: 22162546 PMCID: PMC3295166 12/14/2011
  • Evaluation of a microarray-based genotyping assay for the rapid detection of cytochrome P450 2C19 *2 and *3 polymorphisms from whole blood using nanoparticle probes. (Buchan BW, Peterson JF, Cogbill CH, Anderson DK, Ledford JS, White MN, Quigley NB, Jannetto PJ, Ledeboer NA) Am J Clin Pathol 2011 Oct;136(4):604-8 PMID: 21917683 09/16/2011
  • Identification of two borderline oxacillin-resistant strains of Staphylococcus aureus from routine nares swab specimens by one of three chromogenic agars evaluated for the detection of MRSA. (Buchan BW, Ledeboer NA) Am J Clin Pathol 2010 Dec;134(6):921-7 PMID: 21088156 11/23/2010
  • Real-time detection of influenza a, influenza B, and respiratory syncytial virus a and B in respiratory specimens by use of nanoparticle probes. (Jannetto PJ, Buchan BW, Vaughan KA, Ledford JS, Anderson DK, Henley DC, Quigley NB, Ledeboer NA) J Clin Microbiol 2010 Nov;48(11):3997-4002 PMID: 20826644 PMCID: PMC3020893 09/10/2010
  • Cutting edge: mutation of Francisella tularensis mviN leads to increased macrophage absent in melanoma 2 inflammasome activation and a loss of virulence. (Ulland TK, Buchan BW, Ketterer MR, Fernandes-Alnemri T, Meyerholz DK, Apicella MA, Alnemri ES, Jones BD, Nauseef WM, Sutterwala FS) J Immunol 2010 Sep 1;185(5):2670-4 PMID: 20679532 PMCID: PMC2953561 08/04/2010
  • Multiple mechanisms of NADPH oxidase inhibition by type A and type B Francisella tularensis. (McCaffrey RL, Schwartz JT, Lindemann SR, Moreland JG, Buchan BW, Jones BD, Allen LA) J Leukoc Biol 2010 Oct;88(4):791-805 PMID: 20610796 PMCID: PMC2974429 07/09/2010
  • Identification of migR, a regulatory element of the Francisella tularensis live vaccine strain iglABCD virulence operon required for normal replication and trafficking in macrophages. (Buchan BW, McCaffrey RL, Lindemann SR, Allen LA, Jones BD) Infect Immun 2009 Jun;77(6):2517-29 PMID: 19349423 PMCID: PMC2687360 04/08/2009
  • Francisella tularensis genes required for inhibition of the neutrophil respiratory burst and intramacrophage growth identified by random transposon mutagenesis of strain LVS. (Schulert GS, McCaffrey RL, Buchan BW, Lindemann SR, Hollenback C, Jones BD, Allen LA) Infect Immun 2009 Apr;77(4):1324-36 PMID: 19204089 PMCID: PMC2663180 02/11/2009
  • Identification of differentially regulated francisella tularensis genes by use of a newly developed Tn5-based transposon delivery system. (Buchan BW, McLendon MK, Jones BD) Appl Environ Microbiol 2008 May;74(9):2637-45 PMID: 18344342 PMCID: PMC2394869 03/18/2008
  • Protocols for production of selenomethionine-labeled proteins in 2-L polyethylene terephthalate bottles using auto-induction medium. (Sreenath HK, Bingman CA, Buchan BW, Seder KD, Burns BT, Geetha HV, Jeon WB, Vojtik FC, Aceti DJ, Frederick RO, Phillips GN Jr, Fox BG) Protein Expr Purif 2005 Apr;40(2):256-67 PMID: 15766867 03/16/2005
  • Last update: 07/27/2015