Medical College of Wisconsin
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Chun Liu PhD

Chun Liu PhD profile photo picture

Assistant Professor

Institution: Medical College of Wisconsin
Department: Physiology
Program: Administration

Member of the Cancer Center
Member of the Cardiovascular Center


I am willing to collaborate on research projects.
I am willing to be a peer mentor.

View Curriculum Vitae

Research Areas of Interest

  • CRISPR-Cas Systems
  • Cancer Survivors
  • Cardiovascular Diseases
  • Cell Differentiation
  • Cellular Reprogramming Techniques
  • Epigenomics
  • Genomics
  • Induced Pluripotent Stem Cells
  • Pharmacogenetics
  • Systems Biology

    • Research Experience

  • CRISPR-Cas Systems
  • Cell Differentiation
  • Cellular Reprogramming
  • Endothelial Cells
  • Gene Expression Profiling
  • Induced Pluripotent Stem Cells
  • Myocytes, Cardiac

    • Methodologies and Techniques

  • CRISPR-Cas Systems
  • Cardiovascular Diseases
  • Chromatin Immunoprecipitation
  • High-Throughput Nucleotide Sequencing
  • Induced Pluripotent Stem Cells
  • Sequence Analysis, DNA
  • Sequence Analysis, RNA

    • Publications (38)

  • Studying Long QT Syndrome Caused by NAA10 Genetic Variants Using Patient-Derived Induced Pluripotent Stem Cells. (Belbachir N, Wu Y, Shen M, Zhang SL, Zhang JZ, Liu C, Knollmann BC, Lyon GJ, Ma N, Wu JC) Circulation 2023 Nov 14;148(20):1598-1601 PMID: 37956223 PMCID: PMC10697282 SCOPUS ID: 2-s2.0-85176896789 11/13/2023    
  • Death-seq identifies regulators of cell death and senolytic therapies. (Colville A, Liu JY, Rodriguez-Mateo C, Thomas S, Ishak HD, Zhou R, Klein JDD, Morgens DW, Goshayeshi A, Salvi JS, Yao D, Spees K, Dixon SJ, Liu C, Rhee JW, Lai C, Wu JC, Bassik MC, Rando TA) Cell Metab 2023 Oct 03;35(10):1814-1829.e6 PMID: 37699398 PMCID: PMC10597643 SCOPUS ID: 2-s2.0-85172695750 09/13/2023       3 Citations
  • Statins improve endothelial function via suppression of epigenetic-driven EndMT (Chun Liu, Mengcheng Shen, Wilson L. W. Tan, Ian Y. Chen, Yu Liu, Xuan Yu, Huaxiao Yang, Angela Zhang, Yanxia Liu, Ming-Tao Zhao, Mohamed Ameen, Mao Zhang, Eric R. Gross, Lei S. Qi, Nazish Sayed & Joseph C. Wu) Nature Cardiovascular Research (2023). https://doi.org/10.1038/s44161-023-00267-1 05/08/2023    
  • Protocol to generate cardiac pericytes from human induced pluripotent stem cells. (Shen M, Zhao SR, Khokhar Y, Li L, Zhou Y, Liu C, Wu JC) STAR Protoc 2023 Apr 27;4(2):102256 PMID: 37119139 PMCID: PMC10173856 SCOPUS ID: 2-s2.0-85153537578 04/29/2023    
  • Stepwise Generation of Human Induced Pluripotent Stem Cell-Derived Cardiac Pericytes to Model Coronary Microvascular Dysfunction. (Shen M, Liu C, Zhao SR, Manhas A, Sundaram L, Ameen M, Wu JC) Circulation 2023 Feb 07;147(6):515-518 PMID: 36745700 PMCID: PMC9909621 SCOPUS ID: 2-s2.0-85147460535 02/07/2023       8 Citations
  • Impaired Human Cardiac Cell Development due to NOTCH1 Deficiency. (Ye S, Wang C, Xu Z, Lin H, Wan X, Yu Y, Adhicary S, Zhang JZ, Zhou Y, Liu C, Alonzo M, Bi J, Ramirez-Navarro A, Deschenes I, Ma Q, Garg V, Wu JC, Zhao MT) Circ Res 2023 Jan 20;132(2):187-204 PMID: 36583388 PMCID: PMC9852089 SCOPUS ID: 2-s2.0-85146672196 12/31/2022       12 Citations
  • Cardiotoxicity drug screening based on whole-panel intracellular recording. (Yang Y, Liu A, Tsai CT, Liu C, Wu JC, Cui B) Biosens Bioelectron 2022 Nov 15;216:114617 PMID: 36027802 PMCID: PMC9930661 SCOPUS ID: 2-s2.0-85136501115 08/27/2022       4 Citations
  • Acoustic Fabrication of Living Cardiomyocyte-based Hybrid Biorobots. (Wang J, Soto F, Ma P, Ahmed R, Yang H, Chen S, Wang J, Liu C, Akin D, Fu K, Cao X, Chen P, Hsu EC, Soh HT, Stoyanova T, Wu JC, Demirci U) ACS Nano 2022 Jul 26;16(7):10219-10230 PMID: 35671037 SCOPUS ID: 2-s2.0-85133315697 06/08/2022       12 Citations
  • Generation of Embryonic Origin-Specific Vascular Smooth Muscle Cells from Human Induced Pluripotent Stem Cells. (Shen M, Liu C, Wu JC) Methods Mol Biol 2022;2429:233-246 PMID: 35507165 PMCID: PMC9667909 SCOPUS ID: 2-s2.0-85129368742 05/05/2022       4 Citations
  • Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation. (Wei TT, Chandy M, Nishiga M, Zhang A, Kumar KK, Thomas D, Manhas A, Rhee S, Justesen JM, Chen IY, Wo HT, Khanamiri S, Yang JY, Seidl FJ, Burns NZ, Liu C, Sayed N, Shie JJ, Yeh CF, Yang KC, Lau E, Lynch KL, Rivas M, Kobilka BK, Wu JC) Cell 2022 May 12;185(10):1676-1693.e23 PMID: 35489334 PMCID: PMC9400797 SCOPUS ID: 2-s2.0-85129930623 05/01/2022       42 Citations
  • The use of new CRISPR tools in cardiovascular research and medicine. (Nishiga M, Liu C, Qi LS, Wu JC) Nat Rev Cardiol 2022 Aug;19(8):505-521 PMID: 35145236 PMCID: PMC10283450 SCOPUS ID: 2-s2.0-85124363490 02/12/2022       21 Citations
  • Editorial: Cardiomyocyte Maturation: Novel Insights for Regenerative Medicine. (Zhang M, Qian L, Liu C, Huang GN, Tao G) Front Cell Dev Biol 2021;9:730622 PMID: 34409043 PMCID: PMC8365607 SCOPUS ID: 2-s2.0-85112680869 08/20/2021    
  • Coronary Artery Vasospasm Requiring Cardiac Autotransplantation Yet Controlled With Tobacco. (Tran MV, Marceau E, Liu Y, Sallam K, Medina P, Liu C, Sayed N, Muller MD, Liang DH, Chen IY) JACC Case Rep 2021 Aug 04;3(9):1177-1181 PMID: 34401754 PMCID: PMC8353556 SCOPUS ID: 2-s2.0-85107625647 08/18/2021       1 Citation
  • DUSP26 induces aortic valve calcification by antagonizing MDM2-mediated ubiquitination of DPP4 in human valvular interstitial cells. (Wang Y, Han D, Zhou T, Chen C, Cao H, Zhang JZ, Ma N, Liu C, Song M, Shi J, Jin X, Cao F, Dong N) Eur Heart J 2021 Aug 07;42(30):2935-2951 PMID: 34179958 SCOPUS ID: 2-s2.0-85113772945 06/29/2021       23 Citations
  • A protocol for transdifferentiation of human cardiac fibroblasts into endothelial cells via activation of innate immunity. (Liu C, Medina P, Thomas D, Chen IY, Sallam K, Sayed D, Sayed N) STAR Protoc 2021 Jun 18;2(2):100556 PMID: 34151292 PMCID: PMC8190482 SCOPUS ID: 2-s2.0-85107277970 06/22/2021       2 Citations
  • Antitumor effects of iPSC-based cancer vaccine in pancreatic cancer. (Ouyang X, Liu Y, Zhou Y, Guo J, Wei TT, Liu C, Lee B, Chen B, Zhang A, Casey KM, Wang L, Kooreman NG, Habtezion A, Engleman EG, Wu JC) Stem Cell Reports 2021 Jun 08;16(6):1468-1477 PMID: 33961792 PMCID: PMC8190592 SCOPUS ID: 2-s2.0-85107154080 05/08/2021       24 Citations
  • Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling. (Liu C, Ameen M, Himmati S, Thomas D, Sayed N) Methods Mol Biol 2021;2239:153-162 PMID: 33226618 SCOPUS ID: 2-s2.0-85096805987 11/24/2020       2 Citations
  • Atlas of Exosomal microRNAs Secreted From Human iPSC-Derived Cardiac Cell Types. (Chandy M, Rhee JW, Ozen MO, Williams DR, Pepic L, Liu C, Zhang H, Malisa J, Lau E, Demirci U, Wu JC) Circulation 2020 Nov 03;142(18):1794-1796 PMID: 33136510 PMCID: PMC8135104 SCOPUS ID: 2-s2.0-85095387907 11/03/2020       15 Citations
  • Single-Cell RNA Sequencing Unveils Unique Transcriptomic Signatures of Organ-Specific Endothelial Cells. (Paik DT, Tian L, Williams IM, Rhee S, Zhang H, Liu C, Mishra R, Wu SM, Red-Horse K, Wu JC) Circulation 2020 Nov 10;142(19):1848-1862 PMID: 32929989 PMCID: PMC7658053 SCOPUS ID: 2-s2.0-85095970633 09/16/2020       135 Citations
  • Clinical trial in a dish using iPSCs shows lovastatin improves endothelial dysfunction and cellular cross-talk in LMNA cardiomyopathy. (Sayed N, Liu C, Ameen M, Himmati F, Zhang JZ, Khanamiri S, Moonen JR, Wnorowski A, Cheng L, Rhee JW, Gaddam S, Wang KC, Sallam K, Boyd JH, Woo YJ, Rabinovitch M, Wu JC) Sci Transl Med 2020 Jul 29;12(554) PMID: 32727917 PMCID: PMC7557117 SCOPUS ID: 2-s2.0-85088851582 07/31/2020       57 Citations
  • Melatonin ameliorates aortic valve calcification via the regulation of circular RNA CircRIC3/miR-204-5p/DPP4 signaling in valvular interstitial cells. (Wang Y, Han D, Zhou T, Zhang J, Liu C, Cao F, Dong N) J Pineal Res 2020 Sep;69(2):e12666 PMID: 32369647 SCOPUS ID: 2-s2.0-85085559197 05/06/2020       38 Citations
  • Molecular Signatures of Beneficial Class Effects of Statins on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. (Tian L, Oikonomopoulos A, Liu C, Kitani T, Shrestha R, Chen CL, Ong SG, Smeets M, Karakikes I, Sayed N, Wu JC) Circulation 2020 Apr 07;141(14):1208-1210 PMID: 32250699 PMCID: PMC7170011 SCOPUS ID: 2-s2.0-85083835506 04/07/2020       5 Citations
  • RNA Sequencing Analysis of Induced Pluripotent Stem Cell-Derived Cardiomyocytes From Congenital Heart Disease Patients. (Kitani T, Tian L, Zhang T, Itzhaki I, Zhang JZ, Ma N, Liu C, Rhee JW, Romfh AW, Lui GK, Wu JC) Circ Res 2020 Mar 27;126(7):923-925 PMID: 32070195 PMCID: PMC7123776 SCOPUS ID: 2-s2.0-85082542457 02/20/2020       16 Citations
  • HIF1α Regulates Early Metabolic Changes due to Activation of Innate Immunity in Nuclear Reprogramming. (Liu C, Ruan H, Himmati F, Zhao MT, Chen CC, Makar M, Chen IY, Sallam K, Mocarski ES, Sayed D, Sayed N) Stem Cell Reports 2020 Feb 11;14(2):192-200 PMID: 32048999 PMCID: PMC7013248 SCOPUS ID: 2-s2.0-85079337825 02/13/2020       8 Citations
  • Effects of Spaceflight on Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Structure and Function. (Wnorowski A, Sharma A, Chen H, Wu H, Shao NY, Sayed N, Liu C, Countryman S, Stodieck LS, Rubins KH, Wu SM, Lee PHU, Wu JC) Stem Cell Reports 2019 Dec 10;13(6):960-969 PMID: 31708475 PMCID: PMC6915842 SCOPUS ID: 2-s2.0-85076447528 11/12/2019       56 Citations
  • An in Vivo miRNA Delivery System for Restoring Infarcted Myocardium. (Yang H, Qin X, Wang H, Zhao X, Liu Y, Wo HT, Liu C, Nishiga M, Chen H, Ge J, Sayed N, Abilez OJ, Ding D, Heilshorn SC, Li K) ACS Nano 2019 Sep 24;13(9):9880-9894 PMID: 31149806 PMCID: PMC7930012 SCOPUS ID: 2-s2.0-85067391864 06/01/2019       93 Citations
  • A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles. (Zhang JZ, Termglinchan V, Shao NY, Itzhaki I, Liu C, Ma N, Tian L, Wang VY, Chang ACY, Guo H, Kitani T, Wu H, Lam CK, Kodo K, Sayed N, Blau HM, Wu JC) Cell Stem Cell 2019 May 02;24(5):802-811.e5 PMID: 30880024 PMCID: PMC6499654 SCOPUS ID: 2-s2.0-85063945893 03/19/2019       93 Citations
  • Marked Vascular Dysfunction in a Case of Peripartum Cardiomyopathy. (Khanamiri S, Rhee JW, Paik DT, Chen IY, Liu C, Sayed N) J Vasc Res 2019;56(1):11-15 PMID: 30763932 PMCID: PMC6500747 SCOPUS ID: 2-s2.0-85061607081 02/15/2019       3 Citations
  • Modeling human diseases with induced pluripotent stem cells: from 2D to 3D and beyond. (Liu C, Oikonomopoulos A, Sayed N, Wu JC) Development 2018 Mar 08;145(5) PMID: 29519889 PMCID: PMC5868991 SCOPUS ID: 2-s2.0-85044319384 03/10/2018       179 Citations
  • Molecular and functional resemblance of differentiated cells derived from isogenic human iPSCs and SCNT-derived ESCs. (Zhao MT, Chen H, Liu Q, Shao NY, Sayed N, Wo HT, Zhang JZ, Ong SG, Liu C, Kim Y, Yang H, Chour T, Ma H, Gutierrez NM, Karakikes I, Mitalipov S, Snyder MP, Wu JC) Proc Natl Acad Sci U S A 2017 Dec 26;114(52):E11111-E11120 PMID: 29203658 PMCID: PMC5748177 SCOPUS ID: 2-s2.0-85039707623 12/06/2017       60 Citations
  • Paying the Toll in Nuclear Reprogramming. (Liu C, Himmati F, Sayed N) Front Cell Dev Biol 2017;5:70 PMID: 28861413 PMCID: PMC5562677 SCOPUS ID: 2-s2.0-85028309694 09/02/2017       4 Citations
  • Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics. (Kooreman NG, de Almeida PE, Stack JP, Nelakanti RV, Diecke S, Shao NY, Swijnenburg RJ, Sanchez-Freire V, Matsa E, Liu C, Connolly AJ, Hamming JF, Quax PHA, Brehm MA, Greiner DL, Shultz LD, Wu JC) Cell Rep 2017 Aug 22;20(8):1978-1990 PMID: 28834758 PMCID: PMC5573767 SCOPUS ID: 2-s2.0-85028297667 08/24/2017       28 Citations
  • Transcriptome Profiling of Patient-Specific Human iPSC-Cardiomyocytes Predicts Individual Drug Safety and Efficacy Responses In Vitro. (Matsa E, Burridge PW, Yu KH, Ahrens JH, Termglinchan V, Wu H, Liu C, Shukla P, Sayed N, Churko JM, Shao N, Woo NA, Chao AS, Gold JD, Karakikes I, Snyder MP, Wu JC) Cell Stem Cell 2016 Sep 01;19(3):311-25 PMID: 27545504 PMCID: PMC5087997 SCOPUS ID: 2-s2.0-84991108719 08/23/2016       117 Citations
  • Translation of Human-Induced Pluripotent Stem Cells: From Clinical Trial in a Dish to Precision Medicine. (Sayed N, Liu C, Wu JC) J Am Coll Cardiol 2016 May 10;67(18):2161-2176 PMID: 27151349 PMCID: PMC5086255 SCOPUS ID: 2-s2.0-84966638796 05/07/2016       198 Citations
  • Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. (Hou P, Li Y, Zhang X, Liu C, Guan J, Li H, Zhao T, Ye J, Yang W, Liu K, Ge J, Xu J, Zhang Q, Zhao Y, Deng H) Science 2013 Aug 09;341(6146):651-4 PMID: 23868920 SCOPUS ID: 2-s2.0-84881256653 07/23/2013       1090 Citations
  • Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules. (Li Y, Zhang Q, Yin X, Yang W, Du Y, Hou P, Ge J, Liu C, Zhang W, Zhang X, Wu Y, Li H, Liu K, Wu C, Song Z, Zhao Y, Shi Y, Deng H) Cell Res 2011 Jan;21(1):196-204 PMID: 20956998 PMCID: PMC3193405 SCOPUS ID: 2-s2.0-78650915547 10/20/2010       282 Citations
  • Derivation and characterization of hepatic progenitor cells from human embryonic stem cells. (Zhao D, Chen S, Cai J, Guo Y, Song Z, Che J, Liu C, Wu C, Ding M, Deng H) PLoS One 2009 Jul 31;4(7):e6468 PMID: 19649295 PMCID: PMC2714184 SCOPUS ID: 2-s2.0-68149145797 08/04/2009       91 Citations
  • Two supporting factors greatly improve the efficiency of human iPSC generation. (Zhao Y, Yin X, Qin H, Zhu F, Liu H, Yang W, Zhang Q, Xiang C, Hou P, Song Z, Liu Y, Yong J, Zhang P, Cai J, Liu M, Li H, Li Y, Qu X, Cui K, Zhang W, Xiang T, Wu Y, Zhao Y, Liu C, Yu C, Yuan K, Lou J, Ding M, Deng H) Cell Stem Cell 2008 Nov 06;3(5):475-9 PMID: 18983962 SCOPUS ID: 2-s2.0-54949136146 11/06/2008       404 Citations
    • Last update: 04/14/2024