Medical College of Wisconsin
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Young-In Chi PhD

Young-In Chi PhD profile photo picture

Assistant Professor

Institution: Medical College of Wisconsin
Department: Surgery
Division: Administration
Program: General


Research Experience

  • Crystallography, X-Ray
  • DNA-Binding Proteins
  • Diabetes Mellitus, Type 2
  • Gene Expression Regulation
  • Hepatocyte Nuclear Factor 1
  • Hepatocyte Nuclear Factor 1-alpha
  • Hepatocyte Nuclear Factor 1-beta
  • Hepatocyte Nuclear Factor 4
  • Models, Molecular
  • Molecular Docking Simulation
  • Molecular Structure
  • Mutation
  • Mutation, Missense
  • Nucleic Acid Conformation
  • Protein Binding
  • Protein Conformation
  • Protein Interaction Domains and Motifs
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • RNA
  • Sequence Homology, Amino Acid
  • Transcription Factors
  • Transcription, Genetic
  • Transcriptional Activation
  • Publications (52)

  • Protein lysine 43 methylation by EZH1 promotes AML1-ETO transcriptional repression in leukemia. (Dou L, Yan F, Pang J, Zheng D, Li D, Gao L, Wang L, Xu Y, Shi J, Wang Q, Zhou L, Shen N, Singh P, Wang L, Li Y, Gao Y, Liu T, Chen C, Al-Kali A, Litzow MR, Chi YI, Bode AM, Liu C, Huang H, Liu D, Marcucci G, Liu S, Yu L) Nat Commun 2019 11 07;10(1):5051 PMID: 31699991 PMCID: PMC6838331 11/09/2019    
  • ErbB3-binding protein 1 (EBP1) represses HNF4α-mediated transcription and insulin secretion in pancreatic β-cells. (Han EH, Singh P, Lee IK, Urrutia R, Chi YI) J Biol Chem 2019 09 20;294(38):13983-13994 PMID: 31362984 PMCID: PMC6755798 SCOPUS ID: 2-s2.0-85072530867 08/01/2019       1 Citation
  • Correction to Discovery of Novel Pyruvate Dehydrogenase Kinase 4 Inhibitors for Potential Oral Treatment of Metabolic Diseases. (Lee D, Pagire HS, Pagire SH, Bae EJ, Dighe M, Kim M, Lee KM, Jang YK, Jaladi AK, Jung KY, Yoo EK, Gim HE, Lee S, Choi WI, Chi YI, Song JS, Bae MA, Jeon YH, Lee GH, Liu KH, Lee T, Park S, Jeon JH, Lee IK, Ahn JH) J Med Chem 2019 Mar 14;62(5):2849 PMID: 30811938 02/28/2019    
  • Dimerization defective MODY mutations of hepatocyte nuclear factor 4α. (Singh P, Tung SP, Han EH, Lee IK, Chi YI) Mutat Res 2019 03;814:1-6 PMID: 30648609 SCOPUS ID: 2-s2.0-85059814306 01/17/2019    
  • Discovery of Novel Pyruvate Dehydrogenase Kinase 4 Inhibitors for Potential Oral Treatment of Metabolic Diseases. (Lee D, Pagire HS, Pagire SH, Bae EJ, Dighe M, Kim M, Lee KM, Jang YK, Jaladi AK, Jung KY, Yoo EK, Gim HE, Lee S, Choi WI, Chi YI, Song JS, Bae MA, Jeon YH, Lee GH, Liu KH, Lee T, Park S, Jeon JH, Lee IK, Ahn JH) J Med Chem 2019 01 24;62(2):575-588 PMID: 30623649 01/10/2019       3 Citations
  • Pillars Article: Three-Dimensional Structure of a Human Class II Histocompatibility Molecule Complexed with Superantigen. Nature. 1994. 368: 711-718. (Jardetzky TS, Brown JH, Gorga JC, Stern LJ, Urban RG, Chi YI, Stauffacher C, Strominger JL, Wiley DC) J Immunol 2018 10 01;201(7):1819-1826 PMID: 30224361 09/19/2018    
  • Crystal structures reveal a new and novel FoxO1 binding site within the human glucose-6-phosphatase catalytic subunit 1 gene promoter. (Singh P, Han EH, Endrizzi JA, O'Brien RM, Chi YI) J Struct Biol 2017 04;198(1):54-64 PMID: 28223045 PMCID: PMC5443350 SCOPUS ID: 2-s2.0-85014143554 02/23/2017       5 Citations
  • Dub3 inhibition suppresses breast cancer invasion and metastasis by promoting Snail1 degradation. (Wu Y, Wang Y, Lin Y, Liu Y, Wang Y, Jia J, Singh P, Chi YI, Wang C, Dong C, Li W, Tao M, Napier D, Shi Q, Deng J, Evers BM, Zhou BP) Nat Commun 2017 02 15;8:14228 PMID: 28198361 PMCID: PMC5316870 SCOPUS ID: 2-s2.0-85012981912 02/16/2017       38 Citations
  • Repression of HNF1α-mediated transcription by amino-terminal enhancer of split (AES). (Han EH, Gorman AA, Singh P, Chi YI) Biochem Biophys Res Commun 2015 Dec 4-11;468(1-2):14-20 PMID: 26549228 SCOPUS ID: 2-s2.0-84947714418 11/10/2015       1 Citation
  • Targeting epidermal fatty acid binding protein for treatment of experimental autoimmune encephalomyelitis. (Rao E, Singh P, Li Y, Zhang Y, Chi YI, Suttles J, Li B) BMC Immunol 2015 May 12;16:28 PMID: 25962726 PMCID: PMC4427938 SCOPUS ID: 2-s2.0-84929172594 05/13/2015       7 Citations
  • Inhibition of tumor growth by a newly-identified activator for epidermal fatty acid binding protein. (Rao E, Singh P, Zhai X, Li Y, Zhu G, Zhang Y, Hao J, Chi YI, Brown RE, Cleary MP, Li B) Oncotarget 2015 Apr 10;6(10):7815-27 PMID: 25796556 PMCID: PMC4480718 SCOPUS ID: 2-s2.0-84928393842 03/23/2015       9 Citations
  • Isoliquiritigenin induces apoptosis and inhibits xenograft tumor growth of human lung cancer cells by targeting both wild type and L858R/T790M mutant EGFR. (Jung SK, Lee MH, Lim DY, Kim JE, Singh P, Lee SY, Jeong CH, Lim TG, Chen H, Chi YI, Kundu JK, Lee NH, Lee CC, Cho YY, Bode AM, Lee KW, Dong Z) J Biol Chem 2014 Dec 26;289(52):35839-48 PMID: 25368326 PMCID: PMC4276852 SCOPUS ID: 2-s2.0-84937120687 11/05/2014       57 Citations
  • Crystal structure of 3WJ core revealing divalent ion-promoted thermostability and assembly of the Phi29 hexameric motor pRNA. (Zhang H, Endrizzi JA, Shu Y, Haque F, Sauter C, Shlyakhtenko LS, Lyubchenko Y, Guo P, Chi YI) RNA 2013 Sep;19(9):1226-37 PMID: 23884902 PMCID: PMC3753930 SCOPUS ID: 2-s2.0-84883203974 07/26/2013       70 Citations
  • Specification of hepatopancreas progenitors in zebrafish by hnf1ba and wnt2bb. (Lancman JJ, Zvenigorodsky N, Gates KP, Zhang D, Solomon K, Humphrey RK, Kuo T, Setiawan L, Verkade H, Chi YI, Jhala US, Wright CV, Stainier DY, Dong PD) Development 2013 Jul;140(13):2669-79 PMID: 23720049 PMCID: PMC3678338 SCOPUS ID: 2-s2.0-84878819258 05/31/2013       17 Citations
  • MED25 is a mediator component of HNF4α-driven transcription leading to insulin secretion in pancreatic beta-cells. (Han EH, Rha GB, Chi YI) PLoS One 2012;7(8):e44007 PMID: 22952853 PMCID: PMC3431373 SCOPUS ID: 2-s2.0-84865598963 09/07/2012       9 Citations
  • Interaction with Suv39H1 is critical for Snail-mediated E-cadherin repression in breast cancer. (Dong C, Wu Y, Wang Y, Wang C, Kang T, Rychahou PG, Chi YI, Evers BM, Zhou BP) Oncogene 2013 Mar 14;32(11):1351-62 PMID: 22562246 PMCID: PMC3703513 SCOPUS ID: 2-s2.0-84875220237 05/09/2012       115 Citations
  • Probing the effect of MODY mutations near the co-activator-binding pocket of HNF4α. (Rha GB, Wu G, Chi YI) Biosci Rep 2011 Oct;31(5):411-9 PMID: 21323639 SCOPUS ID: 2-s2.0-80155187983 02/18/2011       3 Citations
  • Solving novel RNA structures using only secondary structural fragments. (Robertson MP, Chi YI, Scott WG) Methods 2010 Oct;52(2):168-72 PMID: 20541014 PMCID: PMC2948636 SCOPUS ID: 2-s2.0-77957127425 06/15/2010       20 Citations
  • The SNAG domain of Snail1 functions as a molecular hook for recruiting lysine-specific demethylase 1. (Lin Y, Wu Y, Li J, Dong C, Ye X, Chi YI, Evers BM, Zhou BP) EMBO J 2010 Jun 02;29(11):1803-16 PMID: 20389281 PMCID: PMC2885925 SCOPUS ID: 2-s2.0-77953107054 04/15/2010       204 Citations
  • The G(0)/G(1) switch gene 2 regulates adipose lipolysis through association with adipose triglyceride lipase. (Yang X, Lu X, Lombès M, Rha GB, Chi YI, Guerin TM, Smart EJ, Liu J) Cell Metab 2010 Mar 03;11(3):194-205 PMID: 20197052 PMCID: PMC3658843 SCOPUS ID: 2-s2.0-77249118270 03/04/2010       276 Citations
  • Multiple binding modes between HNF4alpha and the LXXLL motifs of PGC-1alpha lead to full activation. (Rha GB, Wu G, Shoelson SE, Chi YI) J Biol Chem 2009 Dec 11;284(50):35165-76 PMID: 19846556 PMCID: PMC2787377 SCOPUS ID: 2-s2.0-71749101440 10/23/2009       21 Citations
  • Structure and function of regulatory RNA elements: ribozymes that regulate gene expression. (Scott WG, Martick M, Chi YI) Biochim Biophys Acta 2009 Sep-Oct;1789(9-10):634-41 PMID: 19781673 SCOPUS ID: 2-s2.0-71849111461 09/29/2009       23 Citations
  • Capturing hammerhead ribozyme structures in action by modulating general base catalysis. (Chi YI, Martick M, Lares M, Kim R, Scott WG, Kim SH) PLoS Biol 2008 Sep 30;6(9):e234 PMID: 18834200 PMCID: PMC2553840 SCOPUS ID: 2-s2.0-54749153656 10/07/2008       67 Citations
  • Structural basis of natural promoter recognition by a unique nuclear receptor, HNF4alpha. Diabetes gene product. (Lu P, Rha GB, Melikishvili M, Wu G, Adkins BC, Fried MG, Chi YI) J Biol Chem 2008 Nov 28;283(48):33685-97 PMID: 18829458 PMCID: PMC2586258 SCOPUS ID: 2-s2.0-57749119545 10/03/2008       23 Citations
  • Crystallization of hepatocyte nuclear factor 4 alpha (HNF4 alpha) in complex with the HNF1 alpha promoter element. (Lu P, Liu J, Melikishvili M, Fried MG, Chi YI) Acta Crystallogr Sect F Struct Biol Cryst Commun 2008 Apr 01;64(Pt 4):313-7 PMID: 18391435 PMCID: PMC2374247 SCOPUS ID: 2-s2.0-41949119093 04/09/2008       7 Citations
  • Structural basis of disease-causing mutations in hepatocyte nuclear factor 1beta. (Lu P, Rha GB, Chi YI) Biochemistry 2007 Oct 30;46(43):12071-80 PMID: 17924661 PMCID: PMC2367142 SCOPUS ID: 2-s2.0-35648988438 10/11/2007       23 Citations
  • Crystallization of hepatocyte nuclear factor 1beta in complex with DNA. (Lu P, Li Y, Gorman A, Chi YI) Acta Crystallogr Sect F Struct Biol Cryst Commun 2006 Jun 01;62(Pt 6):525-9 PMID: 16754972 PMCID: PMC1581457 SCOPUS ID: 2-s2.0-33745168228 06/07/2006       4 Citations
  • Homeodomain revisited: a lesson from disease-causing mutations. (Chi YI) Hum Genet 2005 May;116(6):433-44 PMID: 15726414 PMCID: PMC1579204 SCOPUS ID: 2-s2.0-21444446875 02/24/2005       50 Citations
  • A phenylalanine zipper mediates APS dimerization. (Dhe-Paganon S, Werner ED, Nishi M, Hansen L, Chi YI, Shoelson SE) Nat Struct Mol Biol 2004 Oct;11(10):968-74 PMID: 15378031 SCOPUS ID: 2-s2.0-4744363458 09/21/2004       46 Citations
  • Structural basis for HNF-4alpha activation by ligand and coactivator binding. (Duda K, Chi YI, Shoelson SE) J Biol Chem 2004 May 28;279(22):23311-6 PMID: 14982928 SCOPUS ID: 2-s2.0-2542496085 02/26/2004       54 Citations
  • Identification and functional characterization of a novel mutation of hepatocyte nuclear factor-1alpha gene in a Korean family with MODY3. (Kim KA, Kang K, Chi YI, Chang I, Lee MK, Kim KW, Shoelson SE, Lee MS) Diabetologia 2003 May;46(5):721-7 PMID: 12712243 SCOPUS ID: 2-s2.0-0038353162 04/25/2003       18 Citations
  • Diabetes mutations delineate an atypical POU domain in HNF-1alpha. (Chi YI, Frantz JD, Oh BC, Hansen L, Dhe-Paganon S, Shoelson SE) Mol Cell 2002 Nov;10(5):1129-37 PMID: 12453420 SCOPUS ID: 2-s2.0-0036862511 11/28/2002       62 Citations
  • Crystal structure of the HNF4 alpha ligand binding domain in complex with endogenous fatty acid ligand. (Dhe-Paganon S, Duda K, Iwamoto M, Chi YI, Shoelson SE) J Biol Chem 2002 Oct 11;277(41):37973-6 PMID: 12193589 SCOPUS ID: 2-s2.0-0037063997 08/24/2002       208 Citations
  • Zinc-mediated dimerization and its effect on activity and conformation of staphylococcal enterotoxin type C. (Chi YI, Sadler I, Jablonski LM, Callantine SD, Deobald CF, Stauffacher CV, Bohach GA) J Biol Chem 2002 Jun 21;277(25):22839-46 PMID: 11934896 SCOPUS ID: 2-s2.0-0037151107 04/06/2002       26 Citations
  • Structure of the globular tail of nuclear lamin. (Dhe-Paganon S, Werner ED, Chi YI, Shoelson SE) J Biol Chem 2002 May 17;277(20):17381-4 PMID: 11901143 SCOPUS ID: 2-s2.0-0037124053 03/20/2002       192 Citations
  • Crystal structure of human frataxin. (Dhe-Paganon S, Shigeta R, Chi YI, Ristow M, Shoelson SE) J Biol Chem 2000 Oct 06;275(40):30753-6 PMID: 10900192 SCOPUS ID: 2-s2.0-0034613233 07/20/2000       162 Citations
  • X-ray structure of a truncated form of cytochrome f from chlamydomonas reinhardtii. (Chi YI, Huang LS, Zhang Z, Fernández-Velasco JG, Berry EA) Biochemistry 2000 Jul 04;39(26):7689-701 PMID: 10869174 SCOPUS ID: 2-s2.0-0034604237 06/28/2000       43 Citations
  • Crystal structure of the beta-glycosidase from the hyperthermophile Thermosphaera aggregans: insights into its activity and thermostability. (Chi YI, Martinez-Cruz LA, Jancarik J, Swanson RV, Robertson DE, Kim SH) FEBS Lett 1999 Feb 26;445(2-3):375-83 PMID: 10094493 SCOPUS ID: 2-s2.0-0032982023 03/27/1999       65 Citations
  • The structure and function of relationships revealed by mutagenesis in the MHCII binding face of staphylococcal enterotoxin C3 (Stauffacher C, Chi Y, Deobald C, Bohach G) International Congress and Symposium Series - Royal Society of Medicine 1998(229):92 SCOPUS ID: 2-s2.0-33748018155 12/01/1998    
  • Dynamic structure of the mitrochondrial cytochrome bc1complex (Kim SH, Zhang Z, Huang LS, Chi YI, Kim KK, Hung LW, Bcrry KA) FASEB Journal 1998;12(8) SCOPUS ID: 2-s2.0-33749103385 12/01/1998    
  • Structure of an RNA internal loop consisting of tandem C-A+ base pairs. (Jang SB, Hung LW, Chi YI, Holbrook EL, Carter RJ, Holbrook SR) Biochemistry 1998 Aug 25;37(34):11726-31 PMID: 9718295 SCOPUS ID: 2-s2.0-0001475194 08/26/1998       56 Citations
  • Electron transfer by domain movement in cytochrome bc1. (Zhang Z, Huang L, Shulmeister VM, Chi YI, Kim KK, Hung LW, Crofts AR, Berry EA, Kim SH) Nature 1998 Apr 16;392(6677):677-84 PMID: 9565029 SCOPUS ID: 2-s2.0-0032537117 05/16/1998       863 Citations
  • Structure of the redox-active subunits of the mitochondrial cytochrome bc1 complex. variable conformation of the rieske ironsulfur protein (Berrv EA, Huang LS, Zhana Z, Chi YI, Hung LW, Kim SH) FASEB Journal 1997;11(9) SCOPUS ID: 2-s2.0-13144304620 12/01/1997    
  • Apo structure of the ligand-binding domain of aspartate receptor from Escherichia coli and its comparison with ligand-bound or pseudoligand-bound structures. (Chi YI, Yokota H, Kim SH) FEBS Lett 1997 Sep 08;414(2):327-32 PMID: 9315712 SCOPUS ID: 2-s2.0-0031559942 10/07/1997       31 Citations
  • Erratum: Molecular structure of Staphylococcus and Streptococcus superantigens (Journal of Clinical Immunology (1995) Vol. 15 (p. 4S-10S)) (Schlievert PM, Bohach GA, Ohlendorf DH, Stauffacher CV, Leung DYM, Murray DL, Sridhar Prasad G, Earhart CA, Jablonski LM, Hoffmann ML, Chi YI) Journal of Clinical Immunology 1996;16(2):126 SCOPUS ID: 2-s2.0-0029878493 04/15/1996    
  • The staphylococcal and streptococcal pyrogenic toxin family. (Bohach GA, Stauffacher CV, Ohlendorf DH, Chi YI, Vath GM, Schlievert PM) Adv Exp Med Biol 1996;391:131-54 PMID: 8726053 SCOPUS ID: 2-s2.0-0029933748 01/01/1996       23 Citations
  • Molecular structure of staphylococcus and streptococcus superantigens. (Schlievert PM, Bohach GA, Ohlendorf DH, Stauffacher CV, Leung DY, Murray DL, Prasad GS, Earhart CA, Jablonski LM, Hoffmann ML, Chi YI) J Clin Immunol 1995 Nov;15(6 Suppl):4S-10S PMID: 8613491 SCOPUS ID: 2-s2.0-0028822285 11/01/1995       38 Citations
  • Crystallization of HMG-CoA reductase from Pseudomonas mevalonii. (Lawrence CM, Chi YI, Rodwell VW, Stauffacher CV) Acta Crystallogr D Biol Crystallogr 1995 May 01;51(Pt 3):386-9 PMID: 15299306 05/01/1995    
  • Investigation of the role of the disulphide bond in the activity and structure of staphylococcal enterotoxin C1. (Hovde CJ, Marr JC, Hoffmann ML, Hackett SP, Chi YI, Crum KK, Stevens DL, Stauffacher CV, Bohach GA) Mol Microbiol 1994 Sep;13(5):897-909 PMID: 7815947 SCOPUS ID: 2-s2.0-0028104097 09/01/1994       72 Citations
  • Predictions of T-cell receptor- and major histocompatibility complex-binding sites on staphylococcal enterotoxin C1. (Hoffmann ML, Jablonski LM, Crum KK, Hackett SP, Chi YI, Stauffacher CV, Stevens DL, Bohach GA) Infect Immun 1994 Aug;62(8):3396-407 PMID: 8039910 PMCID: PMC302971 SCOPUS ID: 2-s2.0-0027931694 08/01/1994       61 Citations
  • Three-dimensional structure of a human class II histocompatibility molecule complexed with superantigen. (Jardetzky TS, Brown JH, Gorga JC, Stern LJ, Urban RG, Chi YI, Stauffacher C, Strominger JL, Wiley DC) Nature 1994 Apr 21;368(6473):711-8 PMID: 8152483 SCOPUS ID: 2-s2.0-23444454615 04/21/1994       479 Citations
  • Crystallization and preliminary X-ray diffraction analysis of staphylococcal enterotoxin type C. (Bohach GA, Chi YI, Stauffacher CV) Proteins 1992 Apr;13(2):152-7 PMID: 1620697 SCOPUS ID: 2-s2.0-0026691079 04/01/1992       11 Citations
  • Last update: 11/11/2019
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