Medical College of Wisconsin
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Nancy M. Dahms PhD

Nancy M. Dahms PhD

Professor

Institution: Medical College of Wisconsin
Department: Biochemistry

Member of the Cancer Center


Research Experience

  • Fabry Disease
  • Glycoconjugates
  • Lectins
  • Lysosomal Storage Diseases
  • Lysosomes
  • Membrane Glycoproteins
  • Membrane Proteins
  • Organelles
  • Receptor, IGF Type 2
  • Recombinant protein expression systems (Pichia pastoris yeast, Baculovirus insect cells, E. coli, mammalian)
  • Quality control mechanisms in the secretory pathway
  • Receptor, Mannose 6-phosphate
  • Rat model of Fabry Disease
  • Leadership Positions

  • Chair, Radiation Safety Committee 2000-2006
  • Interim Chair, Department of Biochemistry 2009
  • Vice President Faculty Council 2004-2005
  • President Faculty Council 2006-2007
  • Sr Vice President Faculty Council 2005-2006
  • Vice-Chairman, Curriculum and Evaluation Committee 2001-2002
  • Publications

  • α-Galactosidase A-deficient rats accumulate glycosphingolipids and develop cardiorenal phenotypes of Fabry disease. (Miller JJ, Aoki K, Mascari CA, Beltrame AK, Sokumbi O, North PE, Tiemeyer M, Kriegel AJ, Dahms NM) FASEB J 2019 Jan;33(1):418-429 PMID: 29979634 07/07/2018    
  • Neuropathic pain in a Fabry disease rat model. (Miller JJ, Aoki K, Moehring F, Murphy CA, O'Hara CL, Tiemeyer M, Stucky CL, Dahms NM) JCI Insight 2018 Mar 22;3(6) PMID: 29563343 PMCID: PMC5926911 03/23/2018    
  • Cation-Independent Mannose 6-Phosphate Receptor Deficiency Enhances β-Cell Susceptibility to Palmitate. (Baldwin AC, Naatz A, Bohnsack RN, Bartosiak JT, Oleson BJ, Hansen PA, Dahms NM, Corbett JA) Mol Cell Biol 2018 Apr 15;38(8) PMID: 29378831 PMCID: PMC5879465 01/31/2018    
  • Cloning, Expression, and Purification of the Glycosylated Transmembrane Protein, Cation-Dependent Mannose 6-Phosphate Receptor, from Sf9 Cells Using the Baculovirus System. (Olson LJ, Dahms NM) Methods Mol Biol 2018;1722:105-116 PMID: 29264801 PMCID: PMC6202031 12/22/2017    
  • Crystal Structure and Functional Analyses of the Lectin Domain of Glucosidase II: Insights into Oligomannose Recognition. (Olson LJ, Orsi R, Peterson FC, Parodi AJ, Kim JJ, D'Alessio C, Dahms NM) Biochemistry 2015 Jul 07;54(26):4097-111 PMID: 26062005 PMCID: PMC6202027 06/11/2015       7 Citations
  • Bacterial expression of the phosphodiester-binding site of the cation-independent mannose 6-phosphate receptor for crystallographic and NMR studies. (Olson LJ, Jensen DR, Volkman BF, Kim JJ, Peterson FC, Gundry RL, Dahms NM) Protein Expr Purif 2015 Jul;111:91-7 PMID: 25863146 PMCID: PMC4417374 04/12/2015    
  • Glucosidase II and MRH-domain containing proteins in the secretory pathway. (D'Alessio C, Dahms NM) Curr Protein Pept Sci 2015;16(1):31-48 PMID: 25692846 PMCID: PMC4411176 02/19/2015       13 Citations
  • Identification of a fourth mannose 6-phosphate binding site in the cation-independent mannose 6-phosphate receptor. (Olson LJ, Castonguay AC, Lasanajak Y, Peterson FC, Cummings RD, Smith DF, Dahms NM) Glycobiology 2015 Jun;25(6):591-606 PMID: 25573276 PMCID: PMC4410830 01/13/2015       9 Citations
  • Expression of insulin-like growth factor 2 receptor in corneal keratocytes during differentiation and in response to wound healing. (Bohnsack RN, Warejcka DJ, Wang L, Gillespie SR, Bernstein AM, Twining SS, Dahms NM) Invest Ophthalmol Vis Sci 2014 Oct 30;55(12):7697-708 PMID: 25358730 PMCID: PMC4249949 11/02/2014       7 Citations
  • Structure of the lectin mannose 6-phosphate receptor homology (MRH) domain of glucosidase II, an enzyme that regulates glycoprotein folding quality control in the endoplasmic reticulum. (Olson LJ, Orsi R, Alculumbre SG, Peterson FC, Stigliano ID, Parodi AJ, D'Alessio C, Dahms NM) J Biol Chem 2013 Jun 07;288(23):16460-75 PMID: 23609449 PMCID: PMC3675582 04/24/2013       20 Citations
  • Glycosylation-independent lysosomal targeting of acid α-glucosidase enhances muscle glycogen clearance in pompe mice. (Maga JA, Zhou J, Kambampati R, Peng S, Wang X, Bohnsack RN, Thomm A, Golata S, Tom P, Dahms NM, Byrne BJ, LeBowitz JH) J Biol Chem 2013 Jan 18;288(3):1428-38 PMID: 23188827 PMCID: PMC3548456 11/29/2012       45 Citations
  • The glycan-binding properties of the cation-independent mannose 6-phosphate receptor are evolutionary conserved in vertebrates. (Castonguay AC, Lasanajak Y, Song X, Olson LJ, Cummings RD, Smith DF, Dahms NM) Glycobiology 2012 Jul;22(7):983-96 PMID: 22369936 PMCID: PMC3355666 03/01/2012       11 Citations
  • Preparation of a mannose-6-phosphate glycan microarray through fluorescent derivatization, phosphorylation, and immobilization of natural high-mannose N-glycans and application in ligand identification of P-type lectins. (Song X, Heimburg-Molinaro J, Dahms NM, Smith DF, Cummings RD) Methods Mol Biol 2012;808:137-48 PMID: 22057522 PMCID: PMC4668285 11/08/2011       9 Citations
  • Mannose 6-phosphate receptor homology (MRH) domain-containing lectins in the secretory pathway. (Castonguay AC, Olson LJ, Dahms NM) Biochim Biophys Acta 2011 Sep;1810(9):815-26 PMID: 21723917 PMCID: PMC3150748 07/05/2011       22 Citations
  • Structural basis for recognition of phosphodiester-containing lysosomal enzymes by the cation-independent mannose 6-phosphate receptor. (Olson LJ, Peterson FC, Castonguay A, Bohnsack RN, Kudo M, Gotschall RR, Canfield WM, Volkman BF, Dahms NM) Proc Natl Acad Sci U S A 2010 Jul 13;107(28):12493-8 PMID: 20615935 PMCID: PMC2906551 07/10/2010       27 Citations
  • Residues essential for plasminogen binding by the cation-independent mannose 6-phosphate receptor. (Bohnsack RN, Patel M, Olson LJ, Twining SS, Dahms NM) Biochemistry 2010 Jan 26;49(3):635-44 PMID: 20028034 PMCID: PMC2814163 12/24/2009       8 Citations
  • Intermonomer interactions are essential for lysosomal enzyme binding by the cation-dependent mannose 6-phosphate receptor. (Olson LJ, Sun G, Bohnsack RN, Peterson FC, Dahms NM, Kim JJ) Biochemistry 2010 Jan 12;49(1):236-46 PMID: 19928875 PMCID: PMC2802658 11/26/2009       7 Citations
  • Cation-independent mannose 6-phosphate receptor: a composite of distinct phosphomannosyl binding sites. (Bohnsack RN, Song X, Olson LJ, Kudo M, Gotschall RR, Canfield WM, Cummings RD, Smith DF, Dahms NM) J Biol Chem 2009 Dec 11;284(50):35215-26 PMID: 19840944 PMCID: PMC2787381 10/21/2009       38 Citations
  • Carbohydrate recognition by the mannose-6-phosphate receptors. (Kim JJ, Olson LJ, Dahms NM) Curr Opin Struct Biol 2009 Oct;19(5):534-42 PMID: 19801188 PMCID: PMC2771201 10/06/2009       46 Citations
  • Glycan microarray analysis of P-type lectins reveals distinct phosphomannose glycan recognition. (Song X, Lasanajak Y, Olson LJ, Boonen M, Dahms NM, Kornfeld S, Cummings RD, Smith DF) J Biol Chem 2009 Dec 11;284(50):35201-14 PMID: 19801653 PMCID: PMC2787380 10/06/2009       47 Citations
  • Mice lacking mannose 6-phosphate uncovering enzyme activity have a milder phenotype than mice deficient for N-acetylglucosamine-1-phosphotransferase activity. (Boonen M, Vogel P, Platt KA, Dahms N, Kornfeld S) Mol Biol Cell 2009 Oct;20(20):4381-9 PMID: 19710420 PMCID: PMC2762139 08/28/2009       16 Citations
  • Strategies for carbohydrate recognition by the mannose 6-phosphate receptors. (Dahms NM, Olson LJ, Kim JJ) Glycobiology 2008 Sep;18(9):664-78 PMID: 18621992 PMCID: PMC2733771 07/16/2008       65 Citations
  • Structural insights into the mechanism of pH-dependent ligand binding and release by the cation-dependent mannose 6-phosphate receptor. (Olson LJ, Hindsgaul O, Dahms NM, Kim JJ) J Biol Chem 2008 Apr 11;283(15):10124-34 PMID: 18272523 PMCID: PMC2442308 02/15/2008       21 Citations
  • Domain 5 of the cation-independent mannose 6-phosphate receptor preferentially binds phosphodiesters (mannose 6-phosphate N-acetylglucosamine ester). (Chavez CA, Bohnsack RN, Kudo M, Gotschall RR, Canfield WM, Dahms NM) Biochemistry 2007 Nov 06;46(44):12604-17 PMID: 17927214 10/12/2007       36 Citations
  • Identification of residues essential for carbohydrate recognition and cation dependence of the 46-kDa mannose 6-phosphate receptor. (Sun G, Zhao H, Kalyanaraman B, Dahms NM) Glycobiology 2005 Nov;15(11):1136-49 PMID: 15972892 06/24/2005       21 Citations
  • Identification of a low affinity mannose 6-phosphate-binding site in domain 5 of the cation-independent mannose 6-phosphate receptor. (Reddy ST, Chai W, Childs RA, Page JD, Feizi T, Dahms NM) J Biol Chem 2004 Sep 10;279(37):38658-67 PMID: 15252023 07/15/2004       50 Citations
  • The N-terminal carbohydrate recognition site of the cation-independent mannose 6-phosphate receptor. (Olson LJ, Dahms NM, Kim JJ) J Biol Chem 2004 Aug 06;279(32):34000-9 PMID: 15169779 06/01/2004       44 Citations
  • Structure of uPAR, plasminogen, and sugar-binding sites of the 300 kDa mannose 6-phosphate receptor. (Olson LJ, Yammani RD, Dahms NM, Kim JJ) EMBO J 2004 May 19;23(10):2019-28 PMID: 15085180 PMCID: PMC424385 04/16/2004       46 Citations
  • Biochemical and functional properties of the full-length cation-dependent mannose 6-phosphate receptor expressed in Pichia pastoris. (Reddy ST, Kumar SN, Haas AL, Dahms NM) Biochem Biophys Res Commun 2003 Sep 26;309(3):643-51 PMID: 12963039 09/10/2003       7 Citations
  • Transcobalamin II receptor interacts with megalin in the renal apical brush border membrane. (Yammani RR, Seetharam S, Dahms NM, Seetharam B) J Membr Biol 2003 May 01;193(1):57-66 PMID: 12879166 07/25/2003       15 Citations
  • Mannose 6-phosphate receptors: new twists in the tale. (Ghosh P, Dahms NM, Kornfeld S) Nat Rev Mol Cell Biol 2003 Mar;4(3):202-12 PMID: 12612639 03/04/2003       632 Citations
  • High-level expression and characterization of a secreted recombinant cation-dependent mannose 6-phosphate receptor in Pichia pastoris. (Reddy ST, Dahms NM) Protein Expr Purif 2002 Nov;26(2):290-300 PMID: 12406684 10/31/2002       18 Citations
  • Localization of the carbohydrate recognition sites of the insulin-like growth factor II/mannose 6-phosphate receptor to domains 3 and 9 of the extracytoplasmic region. (Hancock MK, Yammani RD, Dahms NM) J Biol Chem 2002 Dec 06;277(49):47205-12 PMID: 12374794 10/11/2002       41 Citations
  • P-type lectins. (Dahms N, Hancock MK) Biochim Biophys Acta 2002 Sep 19;1572(2-3):317-40 PMID: 12223278 09/12/2002       157 Citations
  • Loss of albumin and megalin binding to renal cubilin in rats results in albuminuria after total body irradiation. (Yammani RR, Sharma M, Seetharam S, Moulder JE, Dahms NM, Seetharam B) Am J Physiol Regul Integr Comp Physiol 2002 Aug;283(2):R339-46 PMID: 12121845 07/18/2002       20 Citations
  • Identification of residues essential for carbohydrate recognition by the insulin-like growth factor II/mannose 6-phosphate receptor. (Hancock MK, Haskins DJ, Sun G, Dahms NM) J Biol Chem 2002 Mar 29;277(13):11255-64 PMID: 11799115 01/19/2002       48 Citations
  • Twists and turns of the cation-dependent mannose 6-phosphate receptor. Ligand-bound versus ligand-free receptor. (Olson LJ, Zhang J, Dahms NM, Kim JJ) J Biol Chem 2002 Mar 22;277(12):10156-61 PMID: 11786557 01/12/2002       38 Citations
  • Basolateral sorting signal of the 300-kDa mannose 6-phosphate receptor. (Wick DA, Seetharam B, Dahms NM) Am J Physiol Gastrointest Liver Physiol 2002 Jan;282(1):G51-60 PMID: 11751157 12/26/2001       8 Citations
  • The cation-dependent mannose 6-phosphate receptor. (Kim JJ, Dahms NM) Results Probl Cell Differ 2001;33:39-56 PMID: 11190678 02/24/2001       7 Citations
  • Recognition of Dictyostelium discoideum lysosomal enzymes is conferred by the amino-terminal carbohydrate binding site of the insulin-like growth factor II/mannose 6-phosphate receptor. (Marron-Terada PG, Hancock MK, Haskins DJ, Dahms NM) Biochemistry 2000 Mar 07;39(9):2243-53 PMID: 10694390 03/01/2000       33 Citations
  • Mutational analysis of the binding site residues of the bovine cation-dependent mannose 6-phosphate receptor. (Olson LJ, Hancock MK, Dix D, Kim JJ, Dahms NM) J Biol Chem 1999 Dec 24;274(52):36905-11 PMID: 10601243 12/22/1999       26 Citations
  • Structural basis for recognition of phosphorylated high mannose oligosaccharides by the cation-dependent mannose 6-phosphate receptor. (Olson LJ, Zhang J, Lee YC, Dahms NM, Kim JJ) J Biol Chem 1999 Oct 15;274(42):29889-96 PMID: 10514470 10/09/1999       52 Citations
  • Biosynthesis and secretion of the mannose 6-phosphate receptor and its ligands in polarized Caco-2 cells. (Wick DA, Seetharam B, Dahms NM) Am J Physiol 1999 09;277(3):G506-14 PMID: 10484374 09/14/1999       8 Citations
  • The rate of internalization of the mannose 6-phosphate/insulin-like growth factor II receptor is enhanced by multivalent ligand binding. (York SJ, Arneson LS, Gregory WT, Dahms NM, Kornfeld S) J Biol Chem 1999 Jan 08;274(2):1164-71 PMID: 9873065 01/05/1999       77 Citations
  • Characterization of truncated and glycosylation-deficient forms of the cation-dependent mannose 6-phosphate receptor expressed in baculovirus-infected insect cells. (Marron-Terada PG, Bollinger KE, Dahms NM) Biochemistry 1998 Dec 08;37(49):17223-9 PMID: 9860836 12/23/1998       19 Citations
  • The two mannose 6-phosphate binding sites of the insulin-like growth factor-II/mannose 6-phosphate receptor display different ligand binding properties. (Marron-Terada PG, Brzycki-Wessell MA, Dahms NM) J Biol Chem 1998 Aug 28;273(35):22358-66 PMID: 9712856 08/26/1998       27 Citations
  • Molecular basis of lysosomal enzyme recognition: three-dimensional structure of the cation-dependent mannose 6-phosphate receptor. (Roberts DL, Weix DJ, Dahms NM, Kim JJ) Cell 1998 May 15;93(4):639-48 PMID: 9604938 05/30/1998       99 Citations
  • Bipolar functional expression of transcobalamin II receptor in human intestinal epithelial Caco-2 cells. (Bose S, Seetharam S, Dahms NM, Seetharam B) J Biol Chem 1997 Feb 07;272(6):3538-43 PMID: 9013602 02/07/1997       39 Citations
  • Insulin-like growth factor-II/mannose-6-phosphate receptor expression during early heart development. (McCormick KM, Dahms NM, Lough J) Dev Dyn 1996 Oct;207(2):195-203 PMID: 8906422 10/01/1996       8 Citations
  • Expression of insulin-like growth factor (IGF)-I receptors, IGF-II/cation-independent mannose 6-phosphate receptors (CI-MPRs), and cation-dependent MPRs in polarized human intestinal Caco-2 cells. (Dahms NM, Seetharam B, Wick DA) Biochim Biophys Acta 1996 Feb 21;1279(1):84-92 PMID: 8624366 02/21/1996       12 Citations
  • Insulin-like growth factor II/cation-independent mannose 6-phosphate receptor and lysosomal enzyme recognition. (Dahms NM) Biochem Soc Trans 1996 Feb;24(1):136-41 PMID: 8674625 02/01/1996       36 Citations
  • Expression of IGF-II and IGF binding proteins in differentiating human intestinal Caco-2 cells. (Zhang Y, Wick DA, Seetharam B, Dahms NM) Am J Physiol 1995 Nov;269(5 Pt 1):E804-13 PMID: 7491929 11/01/1995       29 Citations
  • Regulation of lysosomal and ubiquitin degradative pathways in differentiating human intestinal Caco-2 cells. (Zhang Y, Wick DA, Haas AL, Seetharam B, Dahms NM) Biochim Biophys Acta 1995 May 29;1267(1):15-24 PMID: 7540043 05/29/1995       11 Citations
  • Expression and characterization of functional bovine cation-dependent mannose 6-phosphate receptors in baculovirus-infected insect cells. (Dahms NM, Brzycki-Wessell MA) Arch Biochem Biophys 1995 Mar 10;317(2):497-503 PMID: 7893168 03/10/1995    
  • Effect of processing inhibitors on cobalamin (vitamin B12) transcytosis in polarized opossum kidney cells. (Ramanujam KS, Seetharam S, Dahms NM, Seetharam B) Arch Biochem Biophys 1994 Nov 15;315(1):8-15 PMID: 7979409 11/15/1994    
  • The bovine mannose 6-phosphate/insulin-like growth factor II receptor. Localization of the insulin-like growth factor II binding site to domains 5-11. (Dahms NM, Wick DA, Brzycki-Wessell MA) J Biol Chem 1994 Feb 04;269(5):3802-9 PMID: 8106425 02/04/1994       59 Citations
  • Site-directed removal of N-glycosylation sites in the bovine cation-dependent mannose 6-phosphate receptor: effects on ligand binding, intracellular targetting and association with binding immunoglobulin protein. (Zhang Y, Dahms NM) Biochem J 1993 Nov 01;295 ( Pt 3):841-8 PMID: 8240300 PMCID: PMC1134638 11/01/1993       27 Citations
  • Characterization of mannose 6-phosphate receptors (MPRs) from opossum liver: opossum cation-independent MPR binds insulin-like growth factor-II. (Dahms NM, Brzycki-Wessell MA, Ramanujam KS, Seetharam B) Endocrinology 1993 Aug;133(2):440-6 PMID: 8344189 08/01/1993       32 Citations
  • The bovine mannose 6-phosphate/insulin-like growth factor II receptor. The role of arginine residues in mannose 6-phosphate binding. (Dahms NM, Rose PA, Molkentin JD, Zhang Y, Brzycki MA) J Biol Chem 1993 Mar 15;268(8):5457-63 PMID: 8449908 03/15/1993       87 Citations
  • Functional expression of transcobalamin II cDNA in Xenopus laevis oocytes. (Seetharam S, Dahms N, Li N, Seetharam B) Biochem Biophys Res Commun 1991 Dec 31;181(3):1151-5 PMID: 1764066 12/31/1991       7 Citations
  • The bovine mannose 6-phosphate/insulin-like growth factor II receptor. Localization of mannose 6-phosphate binding sites to domains 1-3 and 7-11 of the extracytoplasmic region. (Westlund B, Dahms NM, Kornfeld S) J Biol Chem 1991 Dec 05;266(34):23233-9 PMID: 1660469 12/05/1991       73 Citations
  • Functional expression of intrinsic factor-cobalamin receptor by renal proximal tubular epithelial cells. (Ramanujam KS, Seetharam S, Dahms NM, Seetharam B) J Biol Chem 1991 Jul 15;266(20):13135-40 PMID: 1649178 07/15/1991       27 Citations
  • In vitro translation and expression of renal intrinsic factor-cobalamin receptor. (Seetharam S, Dahms N, Li N, Ramanujam KS, Seetharam B) Biochem Biophys Res Commun 1991 Jun 14;177(2):751-6 PMID: 1646608 06/14/1991       2 Citations
  • Extracellular collagenase, proteoglycanase and products of their activity, released in organ culture by intact dermal inflammatory lesions produced by sulfur mustard. (Woessner JF Jr, Dannenberg AM Jr, Pula PJ, Selzer MG, Ruppert CL, Higuchi K, Kajiki A, Nakamura M, Dahms NM, Kerr JS) J Invest Dermatol 1990 Dec;95(6):717-26 PMID: 2174450 12/01/1990       26 Citations
  • Mannose 6-phosphate receptors and lysosomal enzyme targeting. (Dahms NM, Lobel P, Kornfeld S) J Biol Chem 1989 Jul 25;264(21):12115-8 PMID: 2545698 07/25/1989       335 Citations
  • The cation-dependent mannose 6-phosphate receptor. Structural requirements for mannose 6-phosphate binding and oligomerization. (Dahms NM, Kornfeld S) J Biol Chem 1989 Jul 05;264(19):11458-67 PMID: 2544594 07/05/1989       33 Citations
  • Cloning and sequence analysis of the cation-independent mannose 6-phosphate receptor. (Lobel P, Dahms NM, Kornfeld S) J Biol Chem 1988 Feb 15;263(5):2563-70 PMID: 2963004 02/15/1988       137 Citations
  • 46 kd mannose 6-phosphate receptor: cloning, expression, and homology to the 215 kd mannose 6-phosphate receptor. (Dahms NM, Lobel P, Breitmeyer J, Chirgwin JM, Kornfeld S) Cell 1987 Jul 17;50(2):181-92 PMID: 2954652 07/17/1987       94 Citations
  • Cloning of the bovine 215-kDa cation-independent mannose 6-phosphate receptor. (Lobel P, Dahms NM, Breitmeyer J, Chirgwin JM, Kornfeld S) Proc Natl Acad Sci U S A 1987 Apr;84(8):2233-7 PMID: 2951738 PMCID: PMC304623 04/01/1987       83 Citations
  • Influence of quaternary structure on glycosylation. Differential subunit association affects the site-specific glycosylation of the common beta-chain from Mac-1 and LFA-1. (Dahms NM, Hart GW) J Biol Chem 1986 Oct 05;261(28):13186-96 PMID: 3531197 10/05/1986       51 Citations
  • Lymphocyte function-associated antigen 1 (LFA-1) contains sulfated N-linked oligosaccharides. (Dahms NM, Hart GW) J Immunol 1985 Jun;134(6):3978-86 PMID: 3886793 06/01/1985       22 Citations
  • Ganglioside composition is regulated during differentiation in the neuroblastoma X glioma hybrid cell line NG108-15. (Dahms NM, Schnaar RL) J Neurosci 1983 Apr;3(4):806-17 PMID: 6300357 04/01/1983       58 Citations
  • Last update: 09/05/2018
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