Medical College of Wisconsin
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Fritz Sieber PhD

Fritz Sieber PhD

Professor

Institution: Medical College of Wisconsin
Department: Pediatrics
Division: Hematology/Oncology
Program: Oncology

Member of the MCW Cancer Center


Research Experience

  • Accidents, Radiation
  • Amifostine
  • Antimalarials
  • Antineoplastic Agents, Alkylating
  • Bone Marrow Purging
  • Bone Marrow Transplantation
  • Cell Line, Transformed
  • Cell Line, Tumor
  • Fluorescence
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells
  • Hyperthermia, Induced
  • Light
  • Malaria
  • Models, Animal
  • Organoselenium Compounds
  • Oxidants, Photochemical
  • Photobleaching
  • Photochemotherapy
  • Photosensitizing Agents
  • Phototherapy
  • Plasmodium falciparum
  • Plasmodium yoelii
  • Radiation
  • Radiation Effects
  • Radiation Injuries
  • Radiation Injuries, Experimental
  • Radiation Oncology
  • Radiation, Ionizing
  • Radiation, Nonionizing
  • Radiation-Protective Agents
  • Radiation-Protective Agents
  • Radiation-Sensitizing Agents
  • Reactive Oxygen Species
  • Selenious Acid
  • Selenium
  • Selenium Compounds
  • Singlet Oxygen
  • Sodium Selenite
  • Tumor Cells, Cultured
  • Virus Inactivation
  • Whole-Body Irradiation
  • therapy-induced tissue injury
  • clonal assay
  • photochemical inactivation of protozoan parasites
  • selenium-based interventions
  • selenium-protein conjugates
  • experimental therapeutics
  • merocyanines
  • cytotoxic subnano selenium
  • photochemical inactivation of viruses
  • cytotoxic photoproducts
  • prevention and mitigation of radiation injury
  • anti-cancer agents
  • Clinical Expertise

  • autologous hematopoietic stem cell transplantation
  • photochemical purging
  • ex vivo purging
  • Publications

  • Photochemically generated elemental selenium forms conjugates with serum proteins that are preferentially cytotoxic to leukemia and selected solid tumor cells. (Daziano JP, Günther WH, Krieg M, Tsujino I, Miyagi K, Anderson GS, Sampson RW, Ostrowski MD, Muir SA, Bula RJ, Sieber F) Photochem Photobiol 2012 Mar-Apr;88(2):448-60 PMID 22211823
  • Dietary selenium for the mitigation of radiation injury: effects of selenium dose escalation and timing of supplementation. (Sieber F, Muir SA, Cohen EP, Fish BL, Mäder M, Schock AM, Althouse BJ, Moulder JE) Radiat Res 2011 Sep;176(3):366-74 PMID 21867430
  • Dietary Selenium for the Mitigation of Radiation Injury: Effects of Selenium Dose Escalation and Timing of Supplementation. (Sieber F, Muir SA, Cohen EP, Fish BL, Mäder M, Schock AM, Althouse BJ, Moulder JE) Radiat Res 2011 Jul 8: PMID 21740250
  • Hexaminolevulinate-mediated photodynamic purging of marrow grafts with murine breast carcinoma. (Čunderlíková B, Vasovič V, Sieber F, Furre T, Borgen E, Nesland JM, Peng Q) Bone Marrow Transplant 2011 Aug;46(8):1118-27 PMID 21057550
  • Hexaminolevulinate-mediated photodynamic purging of leukemia cells from BM. (Čunderlíková B, Vasovič V, Sieber F, Furre T, Nesland JM, Peng Q) Bone Marrow Transplant 2010 Oct;45(10):1553-61 PMID 20118993
  • High-dose selenium for the mitigation of radiation injury: a pilot study in a rat model. (Sieber F, Muir SA, Cohen EP, North PE, Fish BL, Irving AA, Mäder M, Moulder JE) Radiat Res 2009 Mar;171(3):368-73 PMID 19267564
  • Potentiation of the antitumor effect of Merocyanine 540-mediated photodynamic therapy by amifostine and amphotericin B. (Tsujino I, Miyagi K, Sampson RW, Sieber F) Photochem Photobiol 2006 Mar-Apr;82(2):458-65 PMID 16613499
  • ELEMENTAL SELENIUM GENERATED BY THE PHOTOBLEACHING OF SELENOMEROCYANINE PHOTOSENSITIZERS FORMS CONJUGATES WITH SERUM MACROMOLECULES THAT ARE TOXIC TO TUMOR CELLS. (Sieber F, Daziano JP, Günther WH, Krieg M, Miyagi K, Sampson RW, Ostrowski MD, Anderson GS, Tsujino I, Bula RJ) Phosphorus Sulfur Silicon Relat Elem 2005;180(3-4):647-657 PMID 16491141
  • Crystal violet combined with Merocyanine 540 for the ex vivo purging of hematopoietic stem cell grafts. (Miyagi K, Sampson RW, Sieber-Blum M, Sieber F) J Photochem Photobiol B 2003 Jul;70(3):133-44 PMID 12962637
  • Preferential inactivation of paediatric solid tumour cells by sequential exposure to Merocyanine 540-mediated photodynamic therapy and Edelfosine: implications for the ex vivo purging of autologous haematopoietic stem cell grafts. (Anderson GS, Tsujino I, Miyagi K, Sampson R, Sieber F) J Photochem Photobiol B 2003 Feb;69(2):87-95 PMID 12633981
  • Anti-tumor effect of Merocyanine 540-mediated photochemotherapy combined with Edelfosine: potential implications for the ex vivo purging of hematopoietic stem cell grafts from breast cancer patients. (Anderson GS, Miyagi K, Sampson RW, Sieber F) J Photochem Photobiol B 2002 Nov;68(2-3):101-8 PMID 12468204
  • Postirradiation hyperthermia selectively potentiates the merocyanine 540-sensitized photoinactivation of small cell lung cancer cells. (Tsujino I, Anderson GS, Sieber F) Photochem Photobiol 2001 Feb;73(2):191-8 PMID 11272734
  • Genetic variability in the response of normal murine hematopoietic progenitor cells to extracorporeal photochemotherapy. (Tsujino I, Sieber F) Photochem Photobiol 2000 Dec;72(6):810-4 PMID 11140270
  • Effect of molecular structure on the performance of triarylmethane dyes as therapeutic agents for photochemical purging of autologous bone marrow grafts from residual tumor cells. (Indig GL, Anderson GS, Nichols MG, Bartlett JA, Mellon WS, Sieber F) J Pharm Sci 2000 Jan;89(1):88-99 PMID 10664541
  • Photochemical purging of autologous bone marrow grafts: assessment of damage to stem cells and the microenvironment in long-term marrow cultures. (Kubo Y, Sieber F) Bone Marrow Transplant 1997 Jul;20(1):27-31 PMID 9232252
  • Role of cytoprotective mechanisms in the photochemical purging of autologous bone marrow grafts. (Yamazaki T, Sato Y, Sieber F) Exp Hematol 1997 Jul;25(7):629-37 PMID 9216739
  • Effect of hypothermia on the merocyanine 540-mediated purging of hematopoietic cells. (Yamazaki T, Sieber F) J Hematother 1997 Feb;6(1):31-9 PMID 9112216
  • The alkyl-lysophospholipid, ET-18-OCH3 synergistically enhances the Merocyanine 540-mediated photoinactivation of leukemia cells: implications for the extracorporeal purging of autologous hematopoietic stem cells. (Yamazaki T, Sieber F) Bone Marrow Transplant 1997 Jan;19(2):113-9 PMID 9116607
  • Inactivation of photosensitizing merocyanine dyes by plasma, serum and serum components. (Anderson GS, Günther WH, Searle R, Bilitz JM, Krieg M, Sieber F) Photochem Photobiol 1996 Oct;64(4):683-7 PMID 8863474
  • Potentiation of merocyanine 540-mediated photodynamic therapy by salicylate and related drugs. (Traul DL, Anderson GS, Bilitz JM, Krieg M, Sieber F) Photochem Photobiol 1995 Oct;62(4):790-9 PMID 7480156
  • Importance of cellular defense mechanisms in the photodynamic purging of autologous bone marrow grafts. (Sieber F, Gaffney DK, Yamazaki T, Qiu K) Prog Clin Biol Res 1994;389:147-54 PMID 7700897
  • Merocyanine 540-sensitized photoinactivation of high-grade non-Hodgkin's lymphoma cells: potential application in autologous BMT. (Itoh T, Messner HA, Jamal N, Tweeddale M, Sieber F) Bone Marrow Transplant 1993 Sep;12(3):191-6 PMID 8241975
  • Phototherapy, photochemotherapy, and bone marrow transplantation. (Sieber F) J Hematother 1993;2(1):43-62 PMID 7921966
  • Photodamaging effects of merocyanine 540 on neutrophils and HL-60 cells. (Smith OM, Traul DL, Sieber F) Exp Hematol 1992 Dec;20(11):1278-84 PMID 1337324
  • The role of serum and serum components in the merocyanine 540-sensitized photoinactivation of K562 leukemia cells. (Gaffney DK, Sieber F) Biochim Biophys Acta 1992 Oct 27;1117(3):321-5 PMID 1420282
  • Merocyanine 540-sensitized photoinactivation of leukemia cells: effects of dose fractionation. (Qiu K, Sieber F) Photochem Photobiol 1992 Oct;56(4):489-93 PMID 1454878
  • Limited cell-cycle dependence of the merocyanine 540-sensitized photoinactivation of L1210 leukemia cells. (Qiu K, Traul DL, Sieber F) Photochem Photobiol 1992 Aug;56(2):277-80 PMID 1502271
  • Merocyanine 540-sensitized photoinactivation of human erythrocytes parasitized by Plasmodium falciparum. (Smith OM, Dolan SA, Dvorak JA, Wellems TE, Sieber F) Blood 1992 Jul 1;80(1):21-4 PMID 1611086
  • Merocyanine 540-sensitized photoinactivation of enveloped viruses in blood products: site and mechanism of phototoxicity. (O'Brien JM, Gaffney DK, Wang TP, Sieber F) Blood 1992 Jul 1;80(1):277-85 PMID 1319237
  • Structure-activity relationships in the antiviral and antileukemic photoproperties of merocyanine dyes. (Günther WH, Searle R, Sieber F) Semin Hematol 1992 Apr;29(2):88-94 PMID 1594949
  • Antiviral effects of photosensitizing merocyanine dyes: implications for transfusion and bone marrow transplantation. (Sieber F, O'Brien JM, Gaffney DK) Semin Hematol 1992 Apr;29(2):79-87 PMID 1594948
  • Introduction: photomedicine--to use the power of light. (Deeg HJ, Sieber F, Porcellini A) Semin Hematol 1992 Apr;29(2):77-8 PMID 1594947
  • Interactions of merocyanine 540 with human brain tumor cells. (Whelan HT, Traul DL, Przybylski C, Segura A, Thomas J, Meyer G, Sieber F) Pediatr Neurol 1992 Mar-Apr;8(2):117-20 PMID 1580954
  • Merocyanine-sensitized photoinactivation of enveloped viruses. (Sieber F, O'Brien JM, Gaffney DK) Blood Cells 1992;18(1):117-27; discussion 128 PMID 1617187
  • Cholesterol content but not plasma membrane fluidity influences the susceptibility of L1210 leukemia cells to merocyanine 540-sensitized irradiation. (Gaffney DK, Feix JB, Schwarz HP, Struve MF, Sieber F) Photochem Photobiol 1991 Nov;54(5):717-23 PMID 1798749
  • Action spectra of the antileukemic and antiviral activities of merocyanine 540. (O'Brien JM, Singh RJ, Feix JB, Kalyanaraman B, Sieber F) Photochem Photobiol 1991 Nov;54(5):851-4 PMID 1665914
  • Plasma membrane properties regulating the sensitivity of leukemia, lymphoma, and solid tumor cells to merocyanine 540-sensitized photoirradiation. (Smith OM, Gaffney DK, Anderson MS, McOlash L, Schober SL, Sieber F) Exp Hematol 1991 Sep;19(8):785-8 PMID 1868893
  • The immunoregulatory effects of merocyanine 540 on in vitro human T- and B-lymphocyte functions. (Lum LG, Yamagami M, Giddings BR, Joshi I, Schober SL, Sensenbrenner LL, Sieber F) Blood 1991 Jun 15;77(12):2701-6 PMID 1646046
  • Evaluation of merocyanine 540-sensitized photoirradiation as a method for purging malarially infected red cells from blood. (Smith OM, Traul DL, McOlash L, Sieber F) J Infect Dis 1991 Jun;163(6):1312-7 PMID 2037797
  • Modulation by thiols of the merocyanine 540-sensitized photolysis of leukemia cells, red cells, and herpes simplex virus type 1. (Gaffney DK, O'Brien JM, Sieber F) Photochem Photobiol 1991 Jan;53(1):85-92 PMID 1851305
  • Merocyanine 540-sensitized photoinactivation of soluble and membrane-bound enzymes in L1210 leukemia cells. (Gaffney DK, Sieber F) Cancer Res 1990 Dec 15;50(24):7765-9 PMID 2174731
  • Evaluation of merocyanine 540-sensitized photoirradiation as a means to inactivate enveloped viruses in blood products. (O'Brien JM, Montgomery RR, Burns WH, Gaffney DK, Sieber F) J Lab Clin Med 1990 Oct;116(4):439-47 PMID 2170551
  • Merocyanine 540-sensitized photoinactivation of leukemia cells: role of oxygen and effects on plasma membrane integrity and mitochondrial respiration. (Gaffney DK, Schober SL, Sieber F) Exp Hematol 1990 Jan;18(1):23-6 PMID 2298265
  • Mutagenicity of merocyanine 540-mediated photosensitization. (O'Brien JM, Sieber F) Exp Hematol 1989 Feb;17(2):166-70 PMID 2912740
  • Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization. (Sieber F, Krueger GJ, O'Brien JM, Schober SL, Sensenbrenner LL, Sharkis SJ) Blood 1989 Jan;73(1):345-50 PMID 2910366
  • Photodynamic therapy and bone marrow transplantation. (Sieber F, Krueger GJ) Semin Hematol 1989 Jan;26(1):35-9 PMID 2646723
  • Merocyanine 540. (Sieber F) Photochem Photobiol 1987 Dec;46(6):1035-42 PMID 3325998
  • Antiviral activity of merocyanine 540. (Sieber F, O'Brien JM, Krueger GJ, Schober SL, Burns WH, Sharkis SJ, Sensenbrenner LL) Photochem Photobiol 1987 Nov;46(5):707-11 PMID 2831550
  • Elimination of residual tumor cells from autologous bone marrow grafts by dye-mediated photolysis: preclinical data. (Sieber F) Photochem Photobiol 1987 Jul;46(1):71-6 PMID 3303074
  • Photodynamic action of merocyanine 540 on artificial and natural cell membranes: involvement of singlet molecular oxygen. (Kalyanaraman B, Feix JB, Sieber F, Thomas JP, Girotti AW) Proc Natl Acad Sci U S A 1987 May;84(9):2999-3003 PMID 3033673
  • Dye-mediated photolysis of normal and neoplastic hematopoietic cells. (Sieber F, Stuart RK, Rowley SD, Sharkis SJ, Sensenbrenner LL) Leuk Res 1987;11(1):43-9 PMID 3807420
  • Dye-mediated photolysis of human neuroblastoma cells: implications for autologous bone marrow transplantation. (Sieber F, Rao S, Rowley SD, Sieber-Blum M) Blood 1986 Jul;68(1):32-6 PMID 3521764
  • Dye-mediated photosensitization of murine neuroblastoma cells. (Sieber F, Sieber-Blum M) Cancer Res 1986 Apr;46(4 Pt 2):2072-6 PMID 3512078
  • Effect of ethanol on thrombopoiesis. (Levine RF, Spivak JL, Meagher RC, Sieber F) Br J Haematol 1986 Feb;62(2):345-54 PMID 3947552
  • Merocyanine 540-mediated photosensitization of leukemia and solid tumor cells. (Sieber F) Prog Clin Biol Res 1985;172B:227-34 PMID 3887425
  • Selective killing of leukemic cells by merocyanine 540-mediated photosensitization. (Sieber F, Spivak JL, Sutcliffe AM) Proc Natl Acad Sci U S A 1984 Dec;81(23):7584-7 PMID 6594700
  • Heterogeneity among early quail neural crest cells. (Sieber-Blum M, Sieber F) Brain Res 1984 Jun;316(2):241-6 PMID 6467014
  • Effects of neuraminidase on the regulation of erythropoiesis. (LaRussa VF, Sieber F, Sensenbrenner LL, Sharkis SJ) Blood 1984 Apr;63(4):784-8 PMID 6608380
  • Susceptibility to merocyanine 540-mediated photosensitization: a differentiation marker on murine hematopoietic progenitor cells. (Meagher RC, Sieber F, Spivak JL) J Cell Physiol 1983 Jul;116(1):118-24 PMID 6602137
  • Fetal hemoglobin production in cultures of primitive and mature human erythroid progenitors: differentiation affects the quantity of fetal hemoglobin produced per fetal-hemoglobin-containing cell. (Dover GJ, Chan T, Sieber F) Blood 1983 Jun;61(6):1242-6 PMID 6188507
  • Modulation of murine erythropoiesis in vitro by syngeneic thymocytes: interactions of enhancing and suppressing subpopulations with fluorescent anti-theta antibody and polyamino acids. (Sieber F, Sharkis SJ) Blood 1982 Oct;60(4):845-50 PMID 7115955
  • Suppression of hematopoietic-progenitor-cell proliferation by ethanol and acetaldehyde. (Meagher RC, Sieber F, Spivak JL) N Engl J Med 1982 Sep 30;307(14):845-9 PMID 7110259
  • Studies on the intercellular adhesion of rat and chicken hepatocytes. Conditions affecting cell-cell specificity. (Kuhlenschmidt MS, Schmell E, Slife CW, Kuhlenschmidt TB, Sieber F, Lee YC, Roseman S) J Biol Chem 1982 Mar 25;257(6):3157-64 PMID 6801042
  • Susceptibility to merocyanine 540-mediated photosensitization as a differentiation marker in murine hematopoietic stem cells. (Sieber F, Meagher RC, Spivak JL) Prog Clin Biol Res 1982;85 Pt B:135-44 PMID 7122563
  • Tumor-promoting phorbol esters stimulate myelopoiesis and suppress erythropoiesis in cultures of mouse bone marrow cells. (Sieber F, Stuart RK, Spivak JL) Proc Natl Acad Sci U S A 1981 Jul;78(7):4402-6 PMID 6945590
  • Quantitative analysis of intercellular adhesive specificity in freshly explanted and cultured cells. (Sieber F, Roseman S) J Cell Biol 1981 Jul;90(1):55-62 PMID 6166626
  • Cellular fibronectin promotes adrenergic differentiation of quail neural crest cells in vitro. (Sieber-Blum M, Sieber F, Yamada KM) Exp Cell Res 1981 Jun;133(2):285-95 PMID 7238602
  • Tumor-promoting phorbol esters promote melanogenesis and prevent expression of the adrenergic phenotype in quail neural crest cells. (Sieber-Blum M, Sieber F) Differentiation 1981;20(2):117-23 PMID 7327315
  • Differential sensitivity of mouse hematopoietic stem cells to merocyanine 540. (Sieber F, Meagher RC, Spivak JL) Differentiation 1981;19(1):65-7 PMID 7327314
  • Chromatography of human urinary erythropoietin and granulocyte colony-stimulating factor on insolubilized phytohaemagglutinin. (Sieber F) Biochim Biophys Acta 1977 Jan 24;496(1):146-54 PMID 300031
  • Erythroid progenitors in mouse bone marrow detected by macroscopic colony formation in culture. (Iscove NN, Sieber F) Exp Hematol 1975 Jan;3(1):32-43 PMID 1157835
  • Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A. (Iscove NN, Sieber F, Winterhalter KH) J Cell Physiol 1974 Apr;83(2):309-20 PMID 4822532
  • Farnesol metabolism in Drosophila melanogaster: ontogeny and tissue distribution of octanol dehydrogenase and aldehyde oxidase. (Madhavan K, Conscience-Egli M, Sieber F, Ursprung H) J Insect Physiol 1973 Jan;19(1):235-41 PMID 4631837
  • Properties of octanol dehydrogenase from Drosophila. (Sieber F, Fox DJ, Ursprung H) FEBS Lett 1972 Oct 1;26(1):274-6 PMID 4636742
  • Grants and Awards

  • Proteinated Subnano Particles of Elemental Selenium for the Treatment of Breast Cancer (#9203427 09/01/2004 - 08/31/2008) Sponsor: DOD Department of Defense
  • Post-Irradiation Intervention to Mitigate and Treat Non* (#9203848 08/31/2005 - 07/31/2012) PI of Nano-Selenium for the Mitigation of Radiation Injuries Post-Irradiation Intervention to Mitigate and Treat Non*-Core B: Pilot Project (#2204374)