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Fritz Sieber PhD

Fritz Sieber PhD

Professor

Institution: Medical College of Wisconsin
Department: Pediatrics
Division: Hematology/Oncology
Program: Oncology

Member of the Cancer Center


Research Experience

  • Accidents, Radiation
  • Amifostine
  • Antimalarials
  • Antineoplastic Agents, Alkylating
  • Bone Marrow Purging
  • Bone Marrow Transplantation
  • Cell Line, Transformed
  • Cell Line, Tumor
  • Fluorescence
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells
  • Hyperthermia, Induced
  • Light
  • Malaria
  • Models, Animal
  • Organoselenium Compounds
  • Oxidants, Photochemical
  • Photobleaching
  • Photochemotherapy
  • Photosensitizing Agents
  • Phototherapy
  • Plasmodium falciparum
  • Plasmodium yoelii
  • Radiation
  • Radiation Effects
  • Radiation Injuries
  • Radiation Injuries, Experimental
  • Radiation Oncology
  • Radiation, Ionizing
  • Radiation, Nonionizing
  • Radiation-Protective Agents
  • Radiation-Protective Agents
  • Radiation-Sensitizing Agents
  • Reactive Oxygen Species
  • Selenious Acid
  • Selenium
  • Selenium Compounds
  • Singlet Oxygen
  • Sodium Selenite
  • Tumor Cells, Cultured
  • Virus Inactivation
  • Whole-Body Irradiation
  • therapy-induced tissue injury
  • clonal assay
  • photochemical inactivation of protozoan parasites
  • selenium-based interventions
  • selenium-protein conjugates
  • experimental therapeutics
  • merocyanines
  • cytotoxic subnano selenium
  • photochemical inactivation of viruses
  • cytotoxic photoproducts
  • prevention and mitigation of radiation injury
  • anti-cancer agents
  • Clinical Expertise

  • autologous hematopoietic stem cell transplantation
  • photochemical purging
  • ex vivo purging
  • Publications

  • Photochemically generated elemental selenium forms conjugates with serum proteins that are preferentially cytotoxic to leukemia and selected solid tumor cells. (Daziano JP, Günther WH, Krieg M, Tsujino I, Miyagi K, Anderson GS, Sampson RW, Ostrowski MD, Muir SA, Bula RJ, Sieber F) Photochem Photobiol 2012 Mar-Apr;88(2):448-60 PMID: 22211823 PMCID: PMC3292634
  • Dietary selenium for the mitigation of radiation injury: effects of selenium dose escalation and timing of supplementation. (Sieber F, Muir SA, Cohen EP, Fish BL, Mäder M, Schock AM, Althouse BJ, Moulder JE) Radiat Res 2011 Sep;176(3):366-74 PMID: 21867430 PMCID: PMC3237945
  • Hexaminolevulinate-mediated photodynamic purging of marrow grafts with murine breast carcinoma. (Čunderlíková B, Vasovič V, Sieber F, Furre T, Borgen E, Nesland JM, Peng Q) Bone Marrow Transplant 2011 Aug;46(8):1118-27 PMID: 21057550
  • Hexaminolevulinate-mediated photodynamic purging of leukemia cells from BM. (Čunderlíková B, Vasovič V, Sieber F, Furre T, Nesland JM, Peng Q) Bone Marrow Transplant 2010 Oct;45(10):1553-61 PMID: 20118993
  • High-dose selenium for the mitigation of radiation injury: a pilot study in a rat model. (Sieber F, Muir SA, Cohen EP, North PE, Fish BL, Irving AA, Mäder M, Moulder JE) Radiat Res 2009 Mar;171(3):368-73 PMID: 19267564 PMCID: PMC2679178
  • Potentiation of the antitumor effect of Merocyanine 540-mediated photodynamic therapy by amifostine and amphotericin B. (Tsujino I, Miyagi K, Sampson RW, Sieber F) Photochem Photobiol 2006 Mar-Apr;82(2):458-65 PMID: 16613499 PMCID: PMC1626648
  • ELEMENTAL SELENIUM GENERATED BY THE PHOTOBLEACHING OF SELENOMEROCYANINE PHOTOSENSITIZERS FORMS CONJUGATES WITH SERUM MACROMOLECULES THAT ARE TOXIC TO TUMOR CELLS. (Sieber F, Daziano JP, Günther WH, Krieg M, Miyagi K, Sampson RW, Ostrowski MD, Anderson GS, Tsujino I, Bula RJ) Phosphorus Sulfur Silicon Relat Elem 2005;180(3-4):647-657 PMID: 16491141 PMCID: PMC1373681
  • Crystal violet combined with Merocyanine 540 for the ex vivo purging of hematopoietic stem cell grafts. (Miyagi K, Sampson RW, Sieber-Blum M, Sieber F) J Photochem Photobiol B 2003 Jul;70(3):133-44 PMID: 12962637
  • Preferential inactivation of paediatric solid tumour cells by sequential exposure to Merocyanine 540-mediated photodynamic therapy and Edelfosine: implications for the ex vivo purging of autologous haematopoietic stem cell grafts. (Anderson GS, Tsujino I, Miyagi K, Sampson R, Sieber F) J Photochem Photobiol B 2003 Feb;69(2):87-95 PMID: 12633981
  • Anti-tumor effect of Merocyanine 540-mediated photochemotherapy combined with Edelfosine: potential implications for the ex vivo purging of hematopoietic stem cell grafts from breast cancer patients. (Anderson GS, Miyagi K, Sampson RW, Sieber F) J Photochem Photobiol B 2002 Nov;68(2-3):101-8 PMID: 12468204
  • Postirradiation hyperthermia selectively potentiates the merocyanine 540-sensitized photoinactivation of small cell lung cancer cells. (Tsujino I, Anderson GS, Sieber F) Photochem Photobiol 2001 Feb;73(2):191-8 PMID: 11272734
  • Genetic variability in the response of normal murine hematopoietic progenitor cells to extracorporeal photochemotherapy. (Tsujino I, Sieber F) Photochem Photobiol 2000 Dec;72(6):810-4 PMID: 11140270
  • Effect of molecular structure on the performance of triarylmethane dyes as therapeutic agents for photochemical purging of autologous bone marrow grafts from residual tumor cells. (Indig GL, Anderson GS, Nichols MG, Bartlett JA, Mellon WS, Sieber F) J Pharm Sci 2000 Jan;89(1):88-99 PMID: 10664541
  • Photochemical purging of autologous bone marrow grafts: assessment of damage to stem cells and the microenvironment in long-term marrow cultures. (Kubo Y, Sieber F) Bone Marrow Transplant 1997 Jul;20(1):27-31 PMID: 9232252
  • Role of cytoprotective mechanisms in the photochemical purging of autologous bone marrow grafts. (Yamazaki T, Sato Y, Sieber F) Exp Hematol 1997 Jul;25(7):629-37 PMID: 9216739
  • Effect of hypothermia on the merocyanine 540-mediated purging of hematopoietic cells. (Yamazaki T, Sieber F) J Hematother 1997 Feb;6(1):31-9 PMID: 9112216
  • The alkyl-lysophospholipid, ET-18-OCH3 synergistically enhances the Merocyanine 540-mediated photoinactivation of leukemia cells: implications for the extracorporeal purging of autologous hematopoietic stem cells. (Yamazaki T, Sieber F) Bone Marrow Transplant 1997 Jan;19(2):113-9 PMID: 9116607
  • Inactivation of photosensitizing merocyanine dyes by plasma, serum and serum components. (Anderson GS, Günther WH, Searle R, Bilitz JM, Krieg M, Sieber F) Photochem Photobiol 1996 Oct;64(4):683-7 PMID: 8863474
  • Potentiation of merocyanine 540-mediated photodynamic therapy by salicylate and related drugs. (Traul DL, Anderson GS, Bilitz JM, Krieg M, Sieber F) Photochem Photobiol 1995 Oct;62(4):790-9 PMID: 7480156
  • Importance of cellular defense mechanisms in the photodynamic purging of autologous bone marrow grafts. (Sieber F, Gaffney DK, Yamazaki T, Qiu K) Prog Clin Biol Res 1994;389:147-54 PMID: 7700897
  • Merocyanine 540-sensitized photoinactivation of high-grade non-Hodgkin's lymphoma cells: potential application in autologous BMT. (Itoh T, Messner HA, Jamal N, Tweeddale M, Sieber F) Bone Marrow Transplant 1993 Sep;12(3):191-6 PMID: 8241975
  • Phototherapy, photochemotherapy, and bone marrow transplantation. (Sieber F) J Hematother 1993;2(1):43-62 PMID: 7921966
  • Photodamaging effects of merocyanine 540 on neutrophils and HL-60 cells. (Smith OM, Traul DL, Sieber F) Exp Hematol 1992 Dec;20(11):1278-84 PMID: 1337324
  • The role of serum and serum components in the merocyanine 540-sensitized photoinactivation of K562 leukemia cells. (Gaffney DK, Sieber F) Biochim Biophys Acta 1992 Oct 27;1117(3):321-5 PMID: 1420282
  • Merocyanine 540-sensitized photoinactivation of leukemia cells: effects of dose fractionation. (Qiu K, Sieber F) Photochem Photobiol 1992 Oct;56(4):489-93 PMID: 1454878
  • Limited cell-cycle dependence of the merocyanine 540-sensitized photoinactivation of L1210 leukemia cells. (Qiu K, Traul DL, Sieber F) Photochem Photobiol 1992 Aug;56(2):277-80 PMID: 1502271
  • Merocyanine 540-sensitized photoinactivation of human erythrocytes parasitized by Plasmodium falciparum. (Smith OM, Dolan SA, Dvorak JA, Wellems TE, Sieber F) Blood 1992 Jul 1;80(1):21-4 PMID: 1611086
  • Merocyanine 540-sensitized photoinactivation of enveloped viruses in blood products: site and mechanism of phototoxicity. (O'Brien JM, Gaffney DK, Wang TP, Sieber F) Blood 1992 Jul 1;80(1):277-85 PMID: 1319237
  • Structure-activity relationships in the antiviral and antileukemic photoproperties of merocyanine dyes. (Günther WH, Searle R, Sieber F) Semin Hematol 1992 Apr;29(2):88-94 PMID: 1594949
  • Antiviral effects of photosensitizing merocyanine dyes: implications for transfusion and bone marrow transplantation. (Sieber F, O'Brien JM, Gaffney DK) Semin Hematol 1992 Apr;29(2):79-87 PMID: 1594948
  • Introduction: photomedicine--to use the power of light. (Deeg HJ, Sieber F, Porcellini A) Semin Hematol 1992 Apr;29(2):77-8 PMID: 1594947
  • Interactions of merocyanine 540 with human brain tumor cells. (Whelan HT, Traul DL, Przybylski C, Segura A, Thomas J, Meyer G, Sieber F) Pediatr Neurol 1992 Mar-Apr;8(2):117-20 PMID: 1580954
  • Merocyanine-sensitized photoinactivation of enveloped viruses. (Sieber F, O'Brien JM, Gaffney DK) Blood Cells 1992;18(1):117-27; discussion 128 PMID: 1617187
  • Cholesterol content but not plasma membrane fluidity influences the susceptibility of L1210 leukemia cells to merocyanine 540-sensitized irradiation. (Gaffney DK, Feix JB, Schwarz HP, Struve MF, Sieber F) Photochem Photobiol 1991 Nov;54(5):717-23 PMID: 1798749
  • Action spectra of the antileukemic and antiviral activities of merocyanine 540. (O'Brien JM, Singh RJ, Feix JB, Kalyanaraman B, Sieber F) Photochem Photobiol 1991 Nov;54(5):851-4 PMID: 1665914
  • Plasma membrane properties regulating the sensitivity of leukemia, lymphoma, and solid tumor cells to merocyanine 540-sensitized photoirradiation. (Smith OM, Gaffney DK, Anderson MS, McOlash L, Schober SL, Sieber F) Exp Hematol 1991 Sep;19(8):785-8 PMID: 1868893
  • The immunoregulatory effects of merocyanine 540 on in vitro human T- and B-lymphocyte functions. (Lum LG, Yamagami M, Giddings BR, Joshi I, Schober SL, Sensenbrenner LL, Sieber F) Blood 1991 Jun 15;77(12):2701-6 PMID: 1646046
  • Evaluation of merocyanine 540-sensitized photoirradiation as a method for purging malarially infected red cells from blood. (Smith OM, Traul DL, McOlash L, Sieber F) J Infect Dis 1991 Jun;163(6):1312-7 PMID: 2037797
  • Modulation by thiols of the merocyanine 540-sensitized photolysis of leukemia cells, red cells, and herpes simplex virus type 1. (Gaffney DK, O'Brien JM, Sieber F) Photochem Photobiol 1991 Jan;53(1):85-92 PMID: 1851305
  • Merocyanine 540-sensitized photoinactivation of soluble and membrane-bound enzymes in L1210 leukemia cells. (Gaffney DK, Sieber F) Cancer Res 1990 Dec 15;50(24):7765-9 PMID: 2174731
  • Evaluation of merocyanine 540-sensitized photoirradiation as a means to inactivate enveloped viruses in blood products. (O'Brien JM, Montgomery RR, Burns WH, Gaffney DK, Sieber F) J Lab Clin Med 1990 Oct;116(4):439-47 PMID: 2170551
  • Merocyanine 540-sensitized photoinactivation of leukemia cells: role of oxygen and effects on plasma membrane integrity and mitochondrial respiration. (Gaffney DK, Schober SL, Sieber F) Exp Hematol 1990 Jan;18(1):23-6 PMID: 2298265
  • Mutagenicity of merocyanine 540-mediated photosensitization. (O'Brien JM, Sieber F) Exp Hematol 1989 Feb;17(2):166-70 PMID: 2912740
  • Inactivation of Friend erythroleukemia virus and Friend virus-transformed cells by merocyanine 540-mediated photosensitization. (Sieber F, Krueger GJ, O'Brien JM, Schober SL, Sensenbrenner LL, Sharkis SJ) Blood 1989 Jan;73(1):345-50 PMID: 2910366
  • Photodynamic therapy and bone marrow transplantation. (Sieber F, Krueger GJ) Semin Hematol 1989 Jan;26(1):35-9 PMID: 2646723
  • Merocyanine 540. (Sieber F) Photochem Photobiol 1987 Dec;46(6):1035-42 PMID: 3325998
  • Antiviral activity of merocyanine 540. (Sieber F, O'Brien JM, Krueger GJ, Schober SL, Burns WH, Sharkis SJ, Sensenbrenner LL) Photochem Photobiol 1987 Nov;46(5):707-11 PMID: 2831550
  • Elimination of residual tumor cells from autologous bone marrow grafts by dye-mediated photolysis: preclinical data. (Sieber F) Photochem Photobiol 1987 Jul;46(1):71-6 PMID: 3303074
  • Photodynamic action of merocyanine 540 on artificial and natural cell membranes: involvement of singlet molecular oxygen. (Kalyanaraman B, Feix JB, Sieber F, Thomas JP, Girotti AW) Proc Natl Acad Sci U S A 1987 May;84(9):2999-3003 PMID: 3033673 PMCID: PMC304788
  • Dye-mediated photolysis of normal and neoplastic hematopoietic cells. (Sieber F, Stuart RK, Rowley SD, Sharkis SJ, Sensenbrenner LL) Leuk Res 1987;11(1):43-9 PMID: 3807420
  • Dye-mediated photolysis of human neuroblastoma cells: implications for autologous bone marrow transplantation. (Sieber F, Rao S, Rowley SD, Sieber-Blum M) Blood 1986 Jul;68(1):32-6 PMID: 3521764
  • Dye-mediated photosensitization of murine neuroblastoma cells. (Sieber F, Sieber-Blum M) Cancer Res 1986 Apr;46(4 Pt 2):2072-6 PMID: 3512078
  • Effect of ethanol on thrombopoiesis. (Levine RF, Spivak JL, Meagher RC, Sieber F) Br J Haematol 1986 Feb;62(2):345-54 PMID: 3947552
  • Merocyanine 540-mediated photosensitization of leukemia and solid tumor cells. (Sieber F) Prog Clin Biol Res 1985;172B:227-34 PMID: 3887425
  • Selective killing of leukemic cells by merocyanine 540-mediated photosensitization. (Sieber F, Spivak JL, Sutcliffe AM) Proc Natl Acad Sci U S A 1984 Dec;81(23):7584-7 PMID: 6594700 PMCID: PMC392191
  • Heterogeneity among early quail neural crest cells. (Sieber-Blum M, Sieber F) Brain Res 1984 Jun;316(2):241-6 PMID: 6467014
  • Effects of neuraminidase on the regulation of erythropoiesis. (LaRussa VF, Sieber F, Sensenbrenner LL, Sharkis SJ) Blood 1984 Apr;63(4):784-8 PMID: 6608380
  • Susceptibility to merocyanine 540-mediated photosensitization: a differentiation marker on murine hematopoietic progenitor cells. (Meagher RC, Sieber F, Spivak JL) J Cell Physiol 1983 Jul;116(1):118-24 PMID: 6602137
  • Fetal hemoglobin production in cultures of primitive and mature human erythroid progenitors: differentiation affects the quantity of fetal hemoglobin produced per fetal-hemoglobin-containing cell. (Dover GJ, Chan T, Sieber F) Blood 1983 Jun;61(6):1242-6 PMID: 6188507
  • Modulation of murine erythropoiesis in vitro by syngeneic thymocytes: interactions of enhancing and suppressing subpopulations with fluorescent anti-theta antibody and polyamino acids. (Sieber F, Sharkis SJ) Blood 1982 Oct;60(4):845-50 PMID: 7115955
  • Suppression of hematopoietic-progenitor-cell proliferation by ethanol and acetaldehyde. (Meagher RC, Sieber F, Spivak JL) N Engl J Med 1982 Sep 30;307(14):845-9 PMID: 7110259
  • Studies on the intercellular adhesion of rat and chicken hepatocytes. Conditions affecting cell-cell specificity. (Kuhlenschmidt MS, Schmell E, Slife CW, Kuhlenschmidt TB, Sieber F, Lee YC, Roseman S) J Biol Chem 1982 Mar 25;257(6):3157-64 PMID: 6801042
  • Susceptibility to merocyanine 540-mediated photosensitization as a differentiation marker in murine hematopoietic stem cells. (Sieber F, Meagher RC, Spivak JL) Prog Clin Biol Res 1982;85 Pt B:135-44 PMID: 7122563
  • Tumor-promoting phorbol esters stimulate myelopoiesis and suppress erythropoiesis in cultures of mouse bone marrow cells. (Sieber F, Stuart RK, Spivak JL) Proc Natl Acad Sci U S A 1981 Jul;78(7):4402-6 PMID: 6945590 PMCID: PMC319798
  • Quantitative analysis of intercellular adhesive specificity in freshly explanted and cultured cells. (Sieber F, Roseman S) J Cell Biol 1981 Jul;90(1):55-62 PMID: 6166626 PMCID: PMC2111821
  • Cellular fibronectin promotes adrenergic differentiation of quail neural crest cells in vitro. (Sieber-Blum M, Sieber F, Yamada KM) Exp Cell Res 1981 Jun;133(2):285-95 PMID: 7238602
  • Tumor-promoting phorbol esters promote melanogenesis and prevent expression of the adrenergic phenotype in quail neural crest cells. (Sieber-Blum M, Sieber F) Differentiation 1981;20(2):117-23 PMID: 7327315
  • Differential sensitivity of mouse hematopoietic stem cells to merocyanine 540. (Sieber F, Meagher RC, Spivak JL) Differentiation 1981;19(1):65-7 PMID: 7327314
  • Chromatography of human urinary erythropoietin and granulocyte colony-stimulating factor on insolubilized phytohaemagglutinin. (Sieber F) Biochim Biophys Acta 1977 Jan 24;496(1):146-54 PMID: 300031
  • Erythroid progenitors in mouse bone marrow detected by macroscopic colony formation in culture. (Iscove NN, Sieber F) Exp Hematol 1975 Jan;3(1):32-43 PMID: 1157835
  • Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A. (Iscove NN, Sieber F, Winterhalter KH) J Cell Physiol 1974 Apr;83(2):309-20 PMID: 4822532
  • Farnesol metabolism in Drosophila melanogaster: ontogeny and tissue distribution of octanol dehydrogenase and aldehyde oxidase. (Madhavan K, Conscience-Egli M, Sieber F, Ursprung H) J Insect Physiol 1973 Jan;19(1):235-41 PMID: 4631837
  • Properties of octanol dehydrogenase from Drosophila. (Sieber F, Fox DJ, Ursprung H) FEBS Lett 1972 Oct 1;26(1):274-6 PMID: 4636742
  • Last update: 03/27/2015